Genome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)

Abstract As one of the largest transcription factor family, basic helix-loop-helix (bHLH) transcription factor family plays an important role in plant metabolism, physiology and growth. Berry color is one of the important factors that determine grape quality. However, the bHLH transcription factor f...

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Autores principales:	Ming Li, Lei Sun, Hong Gu, Dawei Cheng, XiZhi Guo, Rui Chen, Zhiyong Wu, Jianfu Jiang, Xiucai Fan, Jinyong Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c09edc28d134d5da3ee2d570d61e54d
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spelling	oai:doaj.org-article:0c09edc28d134d5da3ee2d570d61e54d2021-12-02T16:36:12ZGenome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)10.1038/s41598-021-85754-w2045-2322https://doaj.org/article/0c09edc28d134d5da3ee2d570d61e54d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85754-whttps://doaj.org/toc/2045-2322Abstract As one of the largest transcription factor family, basic helix-loop-helix (bHLH) transcription factor family plays an important role in plant metabolism, physiology and growth. Berry color is one of the important factors that determine grape quality. However, the bHLH transcription factor family’s function in anthocyanin synthesis of grape berry has not been studied systematically. We identified 115 bHLH transcription factors in grape genome and phylogenetic analysis indicated that bHLH family could be classified into 25 subfamilies. First, we screened six candidate genes by bioinformatics analysis and expression analysis. We found one of the candidate genes VdbHLH037 belonged to III (f) subfamily and interacted with genes related to anthocyanin synthesis through phylogenetic analysis and interaction network prediction. Therefore, we speculated that VdbHLH037 participated in the anthocyanin synthesis process. To confirm this, we transiently expressed VdbHLH037 in grape and Arabidopsis transformation. Compared with the control, transgenic materials can accumulate more anthocyanins. These results provide a good base to study the function of the VdbHLH family in anthocyanin synthesis of grape berry.Ming LiLei SunHong GuDawei ChengXiZhi GuoRui ChenZhiyong WuJianfu JiangXiucai FanJinyong ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ming Li Lei Sun Hong Gu Dawei Cheng XiZhi Guo Rui Chen Zhiyong Wu Jianfu Jiang Xiucai Fan Jinyong Chen Genome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)
description	Abstract As one of the largest transcription factor family, basic helix-loop-helix (bHLH) transcription factor family plays an important role in plant metabolism, physiology and growth. Berry color is one of the important factors that determine grape quality. However, the bHLH transcription factor family’s function in anthocyanin synthesis of grape berry has not been studied systematically. We identified 115 bHLH transcription factors in grape genome and phylogenetic analysis indicated that bHLH family could be classified into 25 subfamilies. First, we screened six candidate genes by bioinformatics analysis and expression analysis. We found one of the candidate genes VdbHLH037 belonged to III (f) subfamily and interacted with genes related to anthocyanin synthesis through phylogenetic analysis and interaction network prediction. Therefore, we speculated that VdbHLH037 participated in the anthocyanin synthesis process. To confirm this, we transiently expressed VdbHLH037 in grape and Arabidopsis transformation. Compared with the control, transgenic materials can accumulate more anthocyanins. These results provide a good base to study the function of the VdbHLH family in anthocyanin synthesis of grape berry.
format	article
author	Ming Li Lei Sun Hong Gu Dawei Cheng XiZhi Guo Rui Chen Zhiyong Wu Jianfu Jiang Xiucai Fan Jinyong Chen
author_facet	Ming Li Lei Sun Hong Gu Dawei Cheng XiZhi Guo Rui Chen Zhiyong Wu Jianfu Jiang Xiucai Fan Jinyong Chen
author_sort	Ming Li
title	Genome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)
title_short	Genome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)
title_full	Genome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)
title_fullStr	Genome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)
title_full_unstemmed	Genome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)
title_sort	genome-wide characterization and analysis of bhlh transcription factors related to anthocyanin biosynthesis in spine grapes (vitis davidii)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0c09edc28d134d5da3ee2d570d61e54d
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Genome-wide characterization and analysis of bHLH transcription factors related to anthocyanin biosynthesis in spine grapes (Vitis davidii)

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