Immunohistochemical Study of Type IV Collagen during Lense Development

BACKGROUND AND OBJECTIVE: Extracellular matrix (ECM) and basement membrane (BM) play important roles in many developmental processes during development and after birth. Among the components of the BM, collagen fibers especially type IV is the most important part of BM. The aim of this study was to d...

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Autores principales:	M Jalali, MR Nikravesh, AA Moeeni, MH Karimfar, SH Mohammadi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2010
Materias:	collagen type iv non fibrillar collagen tropocollagen extracellular matrix lens development lens capsule mouse Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0c103e769d4c47c881db1a5ab43726c1
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spelling	oai:doaj.org-article:0c103e769d4c47c881db1a5ab43726c12021-11-10T09:00:07ZImmunohistochemical Study of Type IV Collagen during Lense Development1561-41072251-7170https://doaj.org/article/0c103e769d4c47c881db1a5ab43726c12010-01-01T00:00:00Zhttp://jbums.org/article-1-3375-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Extracellular matrix (ECM) and basement membrane (BM) play important roles in many developmental processes during development and after birth. Among the components of the BM, collagen fibers especially type IV is the most important part of BM. The aim of this study was to determine expression of collagen type IV in the BM of lens structure during embryonic mouse development.METHODS: In this experimental study, 22 female Balb/C mice were selected randomly and were kept under normal condition, finding vaginal plug was assumed as day zero of pregnancy. From embryonic day 10 to 20, all specimens were sacrificed by cervical dislocation and their head embryos were fixed, serially sectioned and it was also done after birth in 10-20 day neonates. Immunohistochemistry study for tracing of collagen type IV in eye capsule region was carried out. Grading served according to the firth method.FINDINGS: Primary lens was visible in anterior pole of optic cup as an undifferentiated cellular mass on day 11 of gestation. Collagen IV immunostaining appeared at the early stage of embryonic day 12 in BM of anterior epithelial and gradually increased until day 17 in ECM and posterior pole. After this period, severe reaction staining collagen IV was not changed in any part of the lens.CONCLUSION: Our results indicated high levels of collagen IV is present at the BM of anterior epithelial lens during early development (E-12) and increases in anterior and posterior epithelial capsule (E-15 to E-17) days. These findings establish the importance of collagen IV during critical period of developing lens.M Jalali,MR NikraveshAA MoeeniMH Karimfar,SH MohammadiBabol University of Medical Sciencesarticlecollagen type ivnon fibrillar collagentropocollagenextracellular matrixlens developmentlens capsulemouseMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 11, Iss 6, Pp 58-63 (2010)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	collagen type iv non fibrillar collagen tropocollagen extracellular matrix lens development lens capsule mouse Medicine R Medicine (General) R5-920
spellingShingle	collagen type iv non fibrillar collagen tropocollagen extracellular matrix lens development lens capsule mouse Medicine R Medicine (General) R5-920 M Jalali, MR Nikravesh AA Moeeni MH Karimfar, SH Mohammadi Immunohistochemical Study of Type IV Collagen during Lense Development
description	BACKGROUND AND OBJECTIVE: Extracellular matrix (ECM) and basement membrane (BM) play important roles in many developmental processes during development and after birth. Among the components of the BM, collagen fibers especially type IV is the most important part of BM. The aim of this study was to determine expression of collagen type IV in the BM of lens structure during embryonic mouse development.METHODS: In this experimental study, 22 female Balb/C mice were selected randomly and were kept under normal condition, finding vaginal plug was assumed as day zero of pregnancy. From embryonic day 10 to 20, all specimens were sacrificed by cervical dislocation and their head embryos were fixed, serially sectioned and it was also done after birth in 10-20 day neonates. Immunohistochemistry study for tracing of collagen type IV in eye capsule region was carried out. Grading served according to the firth method.FINDINGS: Primary lens was visible in anterior pole of optic cup as an undifferentiated cellular mass on day 11 of gestation. Collagen IV immunostaining appeared at the early stage of embryonic day 12 in BM of anterior epithelial and gradually increased until day 17 in ECM and posterior pole. After this period, severe reaction staining collagen IV was not changed in any part of the lens.CONCLUSION: Our results indicated high levels of collagen IV is present at the BM of anterior epithelial lens during early development (E-12) and increases in anterior and posterior epithelial capsule (E-15 to E-17) days. These findings establish the importance of collagen IV during critical period of developing lens.
format	article
author	M Jalali, MR Nikravesh AA Moeeni MH Karimfar, SH Mohammadi
author_facet	M Jalali, MR Nikravesh AA Moeeni MH Karimfar, SH Mohammadi
author_sort	M Jalali,
title	Immunohistochemical Study of Type IV Collagen during Lense Development
title_short	Immunohistochemical Study of Type IV Collagen during Lense Development
title_full	Immunohistochemical Study of Type IV Collagen during Lense Development
title_fullStr	Immunohistochemical Study of Type IV Collagen during Lense Development
title_full_unstemmed	Immunohistochemical Study of Type IV Collagen during Lense Development
title_sort	immunohistochemical study of type iv collagen during lense development
publisher	Babol University of Medical Sciences
publishDate	2010
url	https://doaj.org/article/0c103e769d4c47c881db1a5ab43726c1
work_keys_str_mv	AT mjalali immunohistochemicalstudyoftypeivcollagenduringlensedevelopment AT mrnikravesh immunohistochemicalstudyoftypeivcollagenduringlensedevelopment AT aamoeeni immunohistochemicalstudyoftypeivcollagenduringlensedevelopment AT mhkarimfar immunohistochemicalstudyoftypeivcollagenduringlensedevelopment AT shmohammadi immunohistochemicalstudyoftypeivcollagenduringlensedevelopment
_version_	1718440255708725248

Immunohistochemical Study of Type IV Collagen during Lense Development

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