Do Rumen <italic toggle="yes">Bacteroidetes</italic> Utilize an Alternative Mechanism for Cellulose Degradation?

ABSTRACT Uncultured and therefore uncharacterized Bacteroidetes lineages are ubiquitous in many natural ecosystems which specialize in lignocellulose degradation. However, their metabolic contribution remains mysterious, as well-studied cultured Bacteroidetes have been shown to degrade only soluble...

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Autores principales:	A. E. Naas, A. K. Mackenzie, J. Mravec, J. Schückel, W. G. T. Willats, V. G. H. Eijsink, P. B. Pope
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0c13abb08ec24869be8680381bc27c41
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spelling	oai:doaj.org-article:0c13abb08ec24869be8680381bc27c412021-11-15T15:47:22ZDo Rumen <italic toggle="yes">Bacteroidetes</italic> Utilize an Alternative Mechanism for Cellulose Degradation?10.1128/mBio.01401-142150-7511https://doaj.org/article/0c13abb08ec24869be8680381bc27c412014-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01401-14https://doaj.org/toc/2150-7511ABSTRACT Uncultured and therefore uncharacterized Bacteroidetes lineages are ubiquitous in many natural ecosystems which specialize in lignocellulose degradation. However, their metabolic contribution remains mysterious, as well-studied cultured Bacteroidetes have been shown to degrade only soluble polysaccharides within the human distal gut and herbivore rumen. We have interrogated a reconstructed genome from an uncultured Bacteroidetes phylotype that dominates a switchgrass-associated community within the cow rumen. Importantly, this characterization effort has revealed the first preliminary evidence for polysaccharide utilization locus (PUL)-catalyzed conversion of cellulose. Based on these findings, we propose a further expansion of the PUL paradigm and the saccharolytic capacity of rumen Bacteroidetes species to include cellulose, the most abundant terrestrial polysaccharide on Earth. Moreover, the perspective of a cellulolytic PUL lays the foundation for PULs to be considered an alternative mechanism for cellulose degradation, next to cellulosomes and free-enzyme systems.A. E. NaasA. K. MackenzieJ. MravecJ. SchückelW. G. T. WillatsV. G. H. EijsinkP. B. PopeAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 4 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 A. E. Naas A. K. Mackenzie J. Mravec J. Schückel W. G. T. Willats V. G. H. Eijsink P. B. Pope Do Rumen <italic toggle="yes">Bacteroidetes</italic> Utilize an Alternative Mechanism for Cellulose Degradation?
description	ABSTRACT Uncultured and therefore uncharacterized Bacteroidetes lineages are ubiquitous in many natural ecosystems which specialize in lignocellulose degradation. However, their metabolic contribution remains mysterious, as well-studied cultured Bacteroidetes have been shown to degrade only soluble polysaccharides within the human distal gut and herbivore rumen. We have interrogated a reconstructed genome from an uncultured Bacteroidetes phylotype that dominates a switchgrass-associated community within the cow rumen. Importantly, this characterization effort has revealed the first preliminary evidence for polysaccharide utilization locus (PUL)-catalyzed conversion of cellulose. Based on these findings, we propose a further expansion of the PUL paradigm and the saccharolytic capacity of rumen Bacteroidetes species to include cellulose, the most abundant terrestrial polysaccharide on Earth. Moreover, the perspective of a cellulolytic PUL lays the foundation for PULs to be considered an alternative mechanism for cellulose degradation, next to cellulosomes and free-enzyme systems.
format	article
author	A. E. Naas A. K. Mackenzie J. Mravec J. Schückel W. G. T. Willats V. G. H. Eijsink P. B. Pope
author_facet	A. E. Naas A. K. Mackenzie J. Mravec J. Schückel W. G. T. Willats V. G. H. Eijsink P. B. Pope
author_sort	A. E. Naas
title	Do Rumen <italic toggle="yes">Bacteroidetes</italic> Utilize an Alternative Mechanism for Cellulose Degradation?
title_short	Do Rumen <italic toggle="yes">Bacteroidetes</italic> Utilize an Alternative Mechanism for Cellulose Degradation?
title_full	Do Rumen <italic toggle="yes">Bacteroidetes</italic> Utilize an Alternative Mechanism for Cellulose Degradation?
title_fullStr	Do Rumen <italic toggle="yes">Bacteroidetes</italic> Utilize an Alternative Mechanism for Cellulose Degradation?
title_full_unstemmed	Do Rumen <italic toggle="yes">Bacteroidetes</italic> Utilize an Alternative Mechanism for Cellulose Degradation?
title_sort	do rumen <italic toggle="yes">bacteroidetes</italic> utilize an alternative mechanism for cellulose degradation?
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/0c13abb08ec24869be8680381bc27c41
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Do Rumen <italic toggle="yes">Bacteroidetes</italic> Utilize an Alternative Mechanism for Cellulose Degradation?

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