High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow

Abstract Megakaryocytes are a rare population of cells that develop in the bone marrow and function to produce platelets that circulate throughout the body and form clots to stop or prevent bleeding. A major challenge in studying megakaryocyte development, and the diseases that arise from their dysf...

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Autores principales:	Lucas M. Bush, Connor P. Healy, James E. Marvin, Tara L. Deans
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c1447b5366c4454aeef49569fc5d692
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spelling	oai:doaj.org-article:0c1447b5366c4454aeef49569fc5d6922021-12-02T14:27:53ZHigh-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow10.1038/s41598-021-87681-22045-2322https://doaj.org/article/0c1447b5366c4454aeef49569fc5d6922021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87681-2https://doaj.org/toc/2045-2322Abstract Megakaryocytes are a rare population of cells that develop in the bone marrow and function to produce platelets that circulate throughout the body and form clots to stop or prevent bleeding. A major challenge in studying megakaryocyte development, and the diseases that arise from their dysfunction, is the identification, classification, and enrichment of megakaryocyte progenitor cells that are produced during hematopoiesis. Here, we present a high throughput strategy for identifying and isolating megakaryocytes and their progenitor cells from a heterogeneous population of bone marrow samples. Specifically, we couple thrombopoietin (TPO) induction, image flow cytometry, and principal component analysis (PCA) to identify and enrich for megakaryocyte progenitor cells that are capable of self-renewal and directly differentiating into mature megakaryocytes. This enrichment strategy distinguishes megakaryocyte progenitors from other lineage-committed cells in a high throughput manner. Furthermore, by using image flow cytometry with PCA, we have identified a combination of markers and characteristics that can be used to isolate megakaryocyte progenitor cells using standard flow cytometry methods. Altogether, these techniques enable the high throughput enrichment and isolation of cells in the megakaryocyte lineage and have the potential to enable rapid disease identification and diagnoses ahead of severe disease progression.Lucas M. BushConnor P. HealyJames E. MarvinTara L. DeansNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucas M. Bush Connor P. Healy James E. Marvin Tara L. Deans High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow
description	Abstract Megakaryocytes are a rare population of cells that develop in the bone marrow and function to produce platelets that circulate throughout the body and form clots to stop or prevent bleeding. A major challenge in studying megakaryocyte development, and the diseases that arise from their dysfunction, is the identification, classification, and enrichment of megakaryocyte progenitor cells that are produced during hematopoiesis. Here, we present a high throughput strategy for identifying and isolating megakaryocytes and their progenitor cells from a heterogeneous population of bone marrow samples. Specifically, we couple thrombopoietin (TPO) induction, image flow cytometry, and principal component analysis (PCA) to identify and enrich for megakaryocyte progenitor cells that are capable of self-renewal and directly differentiating into mature megakaryocytes. This enrichment strategy distinguishes megakaryocyte progenitors from other lineage-committed cells in a high throughput manner. Furthermore, by using image flow cytometry with PCA, we have identified a combination of markers and characteristics that can be used to isolate megakaryocyte progenitor cells using standard flow cytometry methods. Altogether, these techniques enable the high throughput enrichment and isolation of cells in the megakaryocyte lineage and have the potential to enable rapid disease identification and diagnoses ahead of severe disease progression.
format	article
author	Lucas M. Bush Connor P. Healy James E. Marvin Tara L. Deans
author_facet	Lucas M. Bush Connor P. Healy James E. Marvin Tara L. Deans
author_sort	Lucas M. Bush
title	High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow
title_short	High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow
title_full	High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow
title_fullStr	High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow
title_full_unstemmed	High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow
title_sort	high-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0c1447b5366c4454aeef49569fc5d692
work_keys_str_mv	AT lucasmbush highthroughputenrichmentandisolationofmegakaryocyteprogenitorcellsfromthemousebonemarrow AT connorphealy highthroughputenrichmentandisolationofmegakaryocyteprogenitorcellsfromthemousebonemarrow AT jamesemarvin highthroughputenrichmentandisolationofmegakaryocyteprogenitorcellsfromthemousebonemarrow AT taraldeans highthroughputenrichmentandisolationofmegakaryocyteprogenitorcellsfromthemousebonemarrow
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High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow

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