Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.

Streptococcus agalactiae (group B streptococcus or GBS) is a commensal bacterium that can frequently behave as a pathogen, particularly in the neonatal period and in the elderly. The gut is a primary site of GBS colonization and a potential port of entry during neonatal infections caused by hypervir...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Giuseppe Valerio De Gaetano, Germana Lentini, Roberta Galbo, Francesco Coppolino, Agata Famà, Giuseppe Teti, Concetta Beninati
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c15dfc17b604a7f9a31228ae814f945
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0c15dfc17b604a7f9a31228ae814f945
record_format	dspace
spelling	oai:doaj.org-article:0c15dfc17b604a7f9a31228ae814f9452021-12-02T20:10:39ZInvasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.1932-620310.1371/journal.pone.0253242https://doaj.org/article/0c15dfc17b604a7f9a31228ae814f9452021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253242https://doaj.org/toc/1932-6203Streptococcus agalactiae (group B streptococcus or GBS) is a commensal bacterium that can frequently behave as a pathogen, particularly in the neonatal period and in the elderly. The gut is a primary site of GBS colonization and a potential port of entry during neonatal infections caused by hypervirulent clonal complex 17 (CC17) strains. Here we studied the interactions between the prototypical CC17 BM110 strain and polarized enterocytes using the Caco-2 cell line. GBS could adhere to and invade these cells through their apical or basolateral surfaces. Basolateral invasion was considerably more efficient than apical invasion and predominated under conditions resulting in weakening of cell-to-cell junctions. Bacterial internalization occurred by a mechanism involving caveolae- and lipid raft-dependent endocytosis and actin re-organization, but not clathrin-dependent endocytosis. In the first steps of Caco-2 invasion, GBS colocalized with the early endocytic marker EEA-1, to later reside in acidic vacuoles. Taken together, these data suggest that CC17 GBS selectively adheres to the lateral surface of enterocytes from which it enters through caveolar lipid rafts using a classical, actin-dependent endocytic pathway. These data may be useful to develop alternative preventive strategies aimed at blocking GBS invasion of the intestinal barrier.Giuseppe Valerio De GaetanoGermana LentiniRoberta GalboFrancesco CoppolinoAgata FamàGiuseppe TetiConcetta BeninatiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253242 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giuseppe Valerio De Gaetano Germana Lentini Roberta Galbo Francesco Coppolino Agata Famà Giuseppe Teti Concetta Beninati Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.
description	Streptococcus agalactiae (group B streptococcus or GBS) is a commensal bacterium that can frequently behave as a pathogen, particularly in the neonatal period and in the elderly. The gut is a primary site of GBS colonization and a potential port of entry during neonatal infections caused by hypervirulent clonal complex 17 (CC17) strains. Here we studied the interactions between the prototypical CC17 BM110 strain and polarized enterocytes using the Caco-2 cell line. GBS could adhere to and invade these cells through their apical or basolateral surfaces. Basolateral invasion was considerably more efficient than apical invasion and predominated under conditions resulting in weakening of cell-to-cell junctions. Bacterial internalization occurred by a mechanism involving caveolae- and lipid raft-dependent endocytosis and actin re-organization, but not clathrin-dependent endocytosis. In the first steps of Caco-2 invasion, GBS colocalized with the early endocytic marker EEA-1, to later reside in acidic vacuoles. Taken together, these data suggest that CC17 GBS selectively adheres to the lateral surface of enterocytes from which it enters through caveolar lipid rafts using a classical, actin-dependent endocytic pathway. These data may be useful to develop alternative preventive strategies aimed at blocking GBS invasion of the intestinal barrier.
format	article
author	Giuseppe Valerio De Gaetano Germana Lentini Roberta Galbo Francesco Coppolino Agata Famà Giuseppe Teti Concetta Beninati
author_facet	Giuseppe Valerio De Gaetano Germana Lentini Roberta Galbo Francesco Coppolino Agata Famà Giuseppe Teti Concetta Beninati
author_sort	Giuseppe Valerio De Gaetano
title	Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.
title_short	Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.
title_full	Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.
title_fullStr	Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.
title_full_unstemmed	Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.
title_sort	invasion and trafficking of hypervirulent group b streptococci in polarized enterocytes.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0c15dfc17b604a7f9a31228ae814f945
work_keys_str_mv	AT giuseppevaleriodegaetano invasionandtraffickingofhypervirulentgroupbstreptococciinpolarizedenterocytes AT germanalentini invasionandtraffickingofhypervirulentgroupbstreptococciinpolarizedenterocytes AT robertagalbo invasionandtraffickingofhypervirulentgroupbstreptococciinpolarizedenterocytes AT francescocoppolino invasionandtraffickingofhypervirulentgroupbstreptococciinpolarizedenterocytes AT agatafama invasionandtraffickingofhypervirulentgroupbstreptococciinpolarizedenterocytes AT giuseppeteti invasionandtraffickingofhypervirulentgroupbstreptococciinpolarizedenterocytes AT concettabeninati invasionandtraffickingofhypervirulentgroupbstreptococciinpolarizedenterocytes
_version_	1718374926791999488

Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.

Ejemplares similares