Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence

Abstract Qualitative and quantitative display of multiple fluorescent analytes is made simple and reliable in this micelle assisted methodology. The adopted method involves micelle assisted evincing of ppb level of PAHs in water; measurement of total fluorescence (white light excitation fluorescence...

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Autores principales:	John Prakash, Ashok Kumar Mishra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c1726c8c4b74b1087fdf6971f4c9afc
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spelling	oai:doaj.org-article:0c1726c8c4b74b1087fdf6971f4c9afc2021-12-02T15:03:05ZSimultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence10.1038/s41598-020-65788-22045-2322https://doaj.org/article/0c1726c8c4b74b1087fdf6971f4c9afc2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-65788-2https://doaj.org/toc/2045-2322Abstract Qualitative and quantitative display of multiple fluorescent analytes is made simple and reliable in this micelle assisted methodology. The adopted method involves micelle assisted evincing of ppb level of PAHs in water; measurement of total fluorescence (white light excitation fluorescence, WLEF) and data deciphering using multivariate analysis. This protocol yields sensitive and accurate quantification of the cancerous pollutants (PAHs) in aqueous media with Limit of Quantification of the order 1–10 μg/L and accuracy of >98%. The use of WLEF enables the simultaneous acquisition of fluorescence signatures of all the PAHs. It has the additional advantage of being portable, layman-friendly and cost-effective. The optimized amount of surfactants for the simultaneous extraction of PAHs from real samples was estimated as 27.8 mg (19.3 mM) of SDS and 9.1 mg (5 mM) of CTAB. Also, the analytical fidelity of the quantification such as percentage recovery (98 ± 2%), linear dynamic range (2–250 μg/L), RMSEP (<0.5), etc. explains the veracity of methodology.John PrakashAshok Kumar MishraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John Prakash Ashok Kumar Mishra Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence
description	Abstract Qualitative and quantitative display of multiple fluorescent analytes is made simple and reliable in this micelle assisted methodology. The adopted method involves micelle assisted evincing of ppb level of PAHs in water; measurement of total fluorescence (white light excitation fluorescence, WLEF) and data deciphering using multivariate analysis. This protocol yields sensitive and accurate quantification of the cancerous pollutants (PAHs) in aqueous media with Limit of Quantification of the order 1–10 μg/L and accuracy of >98%. The use of WLEF enables the simultaneous acquisition of fluorescence signatures of all the PAHs. It has the additional advantage of being portable, layman-friendly and cost-effective. The optimized amount of surfactants for the simultaneous extraction of PAHs from real samples was estimated as 27.8 mg (19.3 mM) of SDS and 9.1 mg (5 mM) of CTAB. Also, the analytical fidelity of the quantification such as percentage recovery (98 ± 2%), linear dynamic range (2–250 μg/L), RMSEP (<0.5), etc. explains the veracity of methodology.
format	article
author	John Prakash Ashok Kumar Mishra
author_facet	John Prakash Ashok Kumar Mishra
author_sort	John Prakash
title	Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence
title_short	Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence
title_full	Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence
title_fullStr	Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence
title_full_unstemmed	Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence
title_sort	simultaneous quantification of multiple polycyclic aromatic hydrocarbons in aqueous media using micelle assisted white light excitation fluorescence
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0c1726c8c4b74b1087fdf6971f4c9afc
work_keys_str_mv	AT johnprakash simultaneousquantificationofmultiplepolycyclicaromatichydrocarbonsinaqueousmediausingmicelleassistedwhitelightexcitationfluorescence AT ashokkumarmishra simultaneousquantificationofmultiplepolycyclicaromatichydrocarbonsinaqueousmediausingmicelleassistedwhitelightexcitationfluorescence
_version_	1718389059552804864

Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence

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