The Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode

The screen-printed electrodes have gained increasing importance due to their advantages, such as robustness, portability, and easy handling. The manuscript presents the investigation of the interaction between double-strand deoxyribonucleic acid (dsDNA) and three anthracyclines: epirubicin (EPI), id...

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Autores principales:	Leyla Karadurmus, Burcu Dogan-Topal, Sevinc Kurbanoglu, Afzal Shah, Sibel A. Ozkan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanobiosensor idarubicin doxorubicin epirubicin drug–DNA interaction Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/0c21c9809da94619911411ac5a48e84b
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spelling	oai:doaj.org-article:0c21c9809da94619911411ac5a48e84b2021-11-25T18:23:13ZThe Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode10.3390/mi121113372072-666Xhttps://doaj.org/article/0c21c9809da94619911411ac5a48e84b2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1337https://doaj.org/toc/2072-666XThe screen-printed electrodes have gained increasing importance due to their advantages, such as robustness, portability, and easy handling. The manuscript presents the investigation of the interaction between double-strand deoxyribonucleic acid (dsDNA) and three anthracyclines: epirubicin (EPI), idarubicin (IDA), and doxorubicin (DOX) by differential pulse voltammetry on metal nanoparticles modified by screen-printed electrodes. In order to investigate the interaction, the voltammetric signals of dsDNA electroactive bases were used as an indicator. The effect of various metal nanomaterials on the signals of guanine and adenine was evaluated. Moreover, dsDNA/PtNPs/AgNPs/SPE (platinum nanoparticles/silver nanoparticles/screen-printed electrodes) was designed for anthracyclines–dsDNA interaction studies since the layer-by-layer modification strategy of metal nanoparticles increases the surface area. Using the signal of multi-layer calf thymus (ct)-dsDNA, the within-day reproducibility results (RSD%) for guanine and adenine peak currents were found as 0.58% and 0.73%, respectively, and the between-day reproducibility results (RSD%) for guanine and adenine peak currents were found as 1.04% and 1.26%, respectively. The effect of binding time and concentration of three anthracyclines on voltammetric signals of dsDNA bases were also evaluated. The response was examined in the range of 0.3–1.3 ppm EPI, 0.1–1.0 ppm IDA and DOX concentration on dsDNA/PtNPs/AgNPs/SPE. Electrochemical studies proposed that the interaction mechanism between three anthracyclines and dsDNA was an intercalation mode.Leyla KaradurmusBurcu Dogan-TopalSevinc KurbanogluAfzal ShahSibel A. OzkanMDPI AGarticlenanobiosensoridarubicindoxorubicinepirubicindrug–DNA interactionMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1337, p 1337 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanobiosensor idarubicin doxorubicin epirubicin drug–DNA interaction Mechanical engineering and machinery TJ1-1570
spellingShingle	nanobiosensor idarubicin doxorubicin epirubicin drug–DNA interaction Mechanical engineering and machinery TJ1-1570 Leyla Karadurmus Burcu Dogan-Topal Sevinc Kurbanoglu Afzal Shah Sibel A. Ozkan The Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode
description	The screen-printed electrodes have gained increasing importance due to their advantages, such as robustness, portability, and easy handling. The manuscript presents the investigation of the interaction between double-strand deoxyribonucleic acid (dsDNA) and three anthracyclines: epirubicin (EPI), idarubicin (IDA), and doxorubicin (DOX) by differential pulse voltammetry on metal nanoparticles modified by screen-printed electrodes. In order to investigate the interaction, the voltammetric signals of dsDNA electroactive bases were used as an indicator. The effect of various metal nanomaterials on the signals of guanine and adenine was evaluated. Moreover, dsDNA/PtNPs/AgNPs/SPE (platinum nanoparticles/silver nanoparticles/screen-printed electrodes) was designed for anthracyclines–dsDNA interaction studies since the layer-by-layer modification strategy of metal nanoparticles increases the surface area. Using the signal of multi-layer calf thymus (ct)-dsDNA, the within-day reproducibility results (RSD%) for guanine and adenine peak currents were found as 0.58% and 0.73%, respectively, and the between-day reproducibility results (RSD%) for guanine and adenine peak currents were found as 1.04% and 1.26%, respectively. The effect of binding time and concentration of three anthracyclines on voltammetric signals of dsDNA bases were also evaluated. The response was examined in the range of 0.3–1.3 ppm EPI, 0.1–1.0 ppm IDA and DOX concentration on dsDNA/PtNPs/AgNPs/SPE. Electrochemical studies proposed that the interaction mechanism between three anthracyclines and dsDNA was an intercalation mode.
format	article
author	Leyla Karadurmus Burcu Dogan-Topal Sevinc Kurbanoglu Afzal Shah Sibel A. Ozkan
author_facet	Leyla Karadurmus Burcu Dogan-Topal Sevinc Kurbanoglu Afzal Shah Sibel A. Ozkan
author_sort	Leyla Karadurmus
title	The Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode
title_short	The Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode
title_full	The Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode
title_fullStr	The Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode
title_full_unstemmed	The Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode
title_sort	interaction between dna and three intercalating anthracyclines using electrochemical dna nanobiosensor based on metal nanoparticles modified screen-printed electrode
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0c21c9809da94619911411ac5a48e84b
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The Interaction between DNA and Three Intercalating Anthracyclines Using Electrochemical DNA Nanobiosensor Based on Metal Nanoparticles Modified Screen-Printed Electrode

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