Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles

Silver nanoparticles (AgNP) have been increasingly incorporated into food-related and hygiene products for their unique antimicrobial and preservative properties. The consequent oral exposure may then result in unpredicted harmful effects in the gastrointestinal tract (GIT), which should be consider...

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Autores principales:	Ana T. Rufino, Ana Ramalho, Adelaide Sousa, José Miguel P. Ferreira de Oliveira, Paulo Freitas, Manuel A. Gonzalez Gómez, Yolanda Piñeiro-Redondo, José Rivas, Félix Carvalho, Eduarda Fernandes, Marisa Freitas
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	inflammation nanoparticles intestinal cells neutrophils flavonoids Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/0c2285f2ac8c488886f9b80afc590853
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spelling	oai:doaj.org-article:0c2285f2ac8c488886f9b80afc5908532021-11-11T18:34:54ZProtective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles10.3390/molecules262166101420-3049https://doaj.org/article/0c2285f2ac8c488886f9b80afc5908532021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6610https://doaj.org/toc/1420-3049Silver nanoparticles (AgNP) have been increasingly incorporated into food-related and hygiene products for their unique antimicrobial and preservative properties. The consequent oral exposure may then result in unpredicted harmful effects in the gastrointestinal tract (GIT), which should be considered in the risk assessment and risk management of these materials. In the present study, the toxic effects of polyethyleneimine (PEI)-coated AgNP (4 and 19 nm) were evaluated in GIT-relevant cells (Caco-2 cell line as a model of human intestinal cells, and neutrophils as a model of the intestinal inflammatory response). This study also evaluated the putative protective action of dietary flavonoids against such harmful effects. The obtained results showed that AgNP of 4 and 19 nm effectively induced Caco-2 cell death by apoptosis with concomitant production of nitric oxide, irrespective of the size. It was also observed that AgNP induced human neutrophil oxidative burst. Interestingly, some flavonoids, namely quercetin and quercetagetin, prevented the deleterious effects of AgNP in both cell types. Overall, the data of the present study provide a first insight into the promising protective role of flavonoids against the potentially toxic effects of AgNP at the intestinal level.Ana T. RufinoAna RamalhoAdelaide SousaJosé Miguel P. Ferreira de OliveiraPaulo FreitasManuel A. Gonzalez GómezYolanda Piñeiro-RedondoJosé RivasFélix CarvalhoEduarda FernandesMarisa FreitasMDPI AGarticleinflammationnanoparticlesintestinal cellsneutrophilsflavonoidsOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6610, p 6610 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	inflammation nanoparticles intestinal cells neutrophils flavonoids Organic chemistry QD241-441
spellingShingle	inflammation nanoparticles intestinal cells neutrophils flavonoids Organic chemistry QD241-441 Ana T. Rufino Ana Ramalho Adelaide Sousa José Miguel P. Ferreira de Oliveira Paulo Freitas Manuel A. Gonzalez Gómez Yolanda Piñeiro-Redondo José Rivas Félix Carvalho Eduarda Fernandes Marisa Freitas Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles
description	Silver nanoparticles (AgNP) have been increasingly incorporated into food-related and hygiene products for their unique antimicrobial and preservative properties. The consequent oral exposure may then result in unpredicted harmful effects in the gastrointestinal tract (GIT), which should be considered in the risk assessment and risk management of these materials. In the present study, the toxic effects of polyethyleneimine (PEI)-coated AgNP (4 and 19 nm) were evaluated in GIT-relevant cells (Caco-2 cell line as a model of human intestinal cells, and neutrophils as a model of the intestinal inflammatory response). This study also evaluated the putative protective action of dietary flavonoids against such harmful effects. The obtained results showed that AgNP of 4 and 19 nm effectively induced Caco-2 cell death by apoptosis with concomitant production of nitric oxide, irrespective of the size. It was also observed that AgNP induced human neutrophil oxidative burst. Interestingly, some flavonoids, namely quercetin and quercetagetin, prevented the deleterious effects of AgNP in both cell types. Overall, the data of the present study provide a first insight into the promising protective role of flavonoids against the potentially toxic effects of AgNP at the intestinal level.
format	article
author	Ana T. Rufino Ana Ramalho Adelaide Sousa José Miguel P. Ferreira de Oliveira Paulo Freitas Manuel A. Gonzalez Gómez Yolanda Piñeiro-Redondo José Rivas Félix Carvalho Eduarda Fernandes Marisa Freitas
author_facet	Ana T. Rufino Ana Ramalho Adelaide Sousa José Miguel P. Ferreira de Oliveira Paulo Freitas Manuel A. Gonzalez Gómez Yolanda Piñeiro-Redondo José Rivas Félix Carvalho Eduarda Fernandes Marisa Freitas
author_sort	Ana T. Rufino
title	Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles
title_short	Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles
title_full	Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles
title_fullStr	Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles
title_full_unstemmed	Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles
title_sort	protective role of flavonoids against intestinal pro-inflammatory effects of silver nanoparticles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0c2285f2ac8c488886f9b80afc590853
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Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles

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