Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces

Abstract. Iswanto T, Shovitri M, Altway A, Widjaja T, Kusumawati DI, Lisdiyanti P. 2019. Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces. Biodiversitas 20: 1580-1587. The present study deals with the isolation and ident...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	TOTO ISWANTO, MAYA SHOVITRI, ALI ALTWAY, TRI WIDJAJA, DINIHARI INDAH KUSUMAWATI, PUSPITA LISDIYANTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	16s rrna gene sequence, bacteria, caffeine, isolation, identification, phylogenetic analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0c2cf2514cc7477fb20d68f4f2fbd412
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0c2cf2514cc7477fb20d68f4f2fbd412
record_format	dspace
spelling	oai:doaj.org-article:0c2cf2514cc7477fb20d68f4f2fbd4122021-11-21T21:41:27ZIsolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces1412-033X2085-472210.13057/biodiv/d200614https://doaj.org/article/0c2cf2514cc7477fb20d68f4f2fbd4122019-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3563https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Iswanto T, Shovitri M, Altway A, Widjaja T, Kusumawati DI, Lisdiyanti P. 2019. Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces. Biodiversitas 20: 1580-1587. The present study deals with the isolation and identification of caffeine-degrading bacteria obtained from the caffeine contaminated environment or caffeinated wastes. These bacteria are useful for various biotechnological applications especially in increasing the potential utilization of caffeinated wastes and producing the high-value chemicals. The suspected caffeine-degrading bacteria have been isolated from the soil of coffee plantation area, coffee pulp waste, and the excreted coffee bean in fresh feces of Luwak (Paradoxurus hermaphroditus or Asian Palm Civet) by growing them on the caffeinated agar medium (CAM) containing basal salt medium (M9) and caffeine as a sole source of carbon and nitrogen. CAM-supplemented with 1.5 to 10 g L-1 of caffeine has been used for screening of the potential bacteria which able to grow in high caffeine concentration. Molecular identification based on 16S rRNA gene sequence was performed to identify the selected bacteria. The result revealed that there were 11 and 3 strains of 12 selected bacteria which could grow on the CAM-supplemented with caffeine up to 7 and 10 g L-1, respectively. Based on 16S rRNA gene sequence and phylogenetic analysis, those bacteria were from 5 Gram-negative species, namely Pseudomonas japonica (4/12), Methylobacterium populi (5/12), Raoultella ornithinolytica (1/12), Klebsiella quasipneumoniae (1/12), and Stenotrophomonas chelatiphaga (1/12). Further investigations to determine their metabolic pathway, enzyme, and growth kinetics in the caffeinated medium may provide insights into its possible utilization for scientific or other applications.TOTO ISWANTOMAYA SHOVITRIALI ALTWAYTRI WIDJAJADINIHARI INDAH KUSUMAWATIPUSPITA LISDIYANTIMBI & UNS Soloarticle16s rrna gene sequence, bacteria, caffeine, isolation, identification, phylogenetic analysisBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 6 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna gene sequence, bacteria, caffeine, isolation, identification, phylogenetic analysis Biology (General) QH301-705.5
spellingShingle	16s rrna gene sequence, bacteria, caffeine, isolation, identification, phylogenetic analysis Biology (General) QH301-705.5 TOTO ISWANTO MAYA SHOVITRI ALI ALTWAY TRI WIDJAJA DINIHARI INDAH KUSUMAWATI PUSPITA LISDIYANTI Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces
description	Abstract. Iswanto T, Shovitri M, Altway A, Widjaja T, Kusumawati DI, Lisdiyanti P. 2019. Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces. Biodiversitas 20: 1580-1587. The present study deals with the isolation and identification of caffeine-degrading bacteria obtained from the caffeine contaminated environment or caffeinated wastes. These bacteria are useful for various biotechnological applications especially in increasing the potential utilization of caffeinated wastes and producing the high-value chemicals. The suspected caffeine-degrading bacteria have been isolated from the soil of coffee plantation area, coffee pulp waste, and the excreted coffee bean in fresh feces of Luwak (Paradoxurus hermaphroditus or Asian Palm Civet) by growing them on the caffeinated agar medium (CAM) containing basal salt medium (M9) and caffeine as a sole source of carbon and nitrogen. CAM-supplemented with 1.5 to 10 g L-1 of caffeine has been used for screening of the potential bacteria which able to grow in high caffeine concentration. Molecular identification based on 16S rRNA gene sequence was performed to identify the selected bacteria. The result revealed that there were 11 and 3 strains of 12 selected bacteria which could grow on the CAM-supplemented with caffeine up to 7 and 10 g L-1, respectively. Based on 16S rRNA gene sequence and phylogenetic analysis, those bacteria were from 5 Gram-negative species, namely Pseudomonas japonica (4/12), Methylobacterium populi (5/12), Raoultella ornithinolytica (1/12), Klebsiella quasipneumoniae (1/12), and Stenotrophomonas chelatiphaga (1/12). Further investigations to determine their metabolic pathway, enzyme, and growth kinetics in the caffeinated medium may provide insights into its possible utilization for scientific or other applications.
format	article
author	TOTO ISWANTO MAYA SHOVITRI ALI ALTWAY TRI WIDJAJA DINIHARI INDAH KUSUMAWATI PUSPITA LISDIYANTI
author_facet	TOTO ISWANTO MAYA SHOVITRI ALI ALTWAY TRI WIDJAJA DINIHARI INDAH KUSUMAWATI PUSPITA LISDIYANTI
author_sort	TOTO ISWANTO
title	Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces
title_short	Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces
title_full	Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces
title_fullStr	Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces
title_full_unstemmed	Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces
title_sort	isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in luwak feces
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/0c2cf2514cc7477fb20d68f4f2fbd412
work_keys_str_mv	AT totoiswanto isolationandidentificationofcaffeinedegradingbacteriafromsoilcoffeepulpwasteandexcretedcoffeebeaninluwakfeces AT mayashovitri isolationandidentificationofcaffeinedegradingbacteriafromsoilcoffeepulpwasteandexcretedcoffeebeaninluwakfeces AT alialtway isolationandidentificationofcaffeinedegradingbacteriafromsoilcoffeepulpwasteandexcretedcoffeebeaninluwakfeces AT triwidjaja isolationandidentificationofcaffeinedegradingbacteriafromsoilcoffeepulpwasteandexcretedcoffeebeaninluwakfeces AT dinihariindahkusumawati isolationandidentificationofcaffeinedegradingbacteriafromsoilcoffeepulpwasteandexcretedcoffeebeaninluwakfeces AT puspitalisdiyanti isolationandidentificationofcaffeinedegradingbacteriafromsoilcoffeepulpwasteandexcretedcoffeebeaninluwakfeces
_version_	1718418624203456512

Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces

Ejemplares similares