Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers

Ayseg&uuml;l Doğan,1 Mehmet E Yalva&ccedil;,1,2 Fikrettin Şahin,1 Alexander V Kabanov,3&ndash;5 Andr&aacute;s Palot&aacute;s,6 Albert A Rizvanov71Department of Genetics and BioEngineering, College of Engineering and Architecture, Yeditepe University, Istanbul,...

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Autores principales:	Doğan A, Şahin F, Kabanov AV, Palot&aacute;s A, Rizvanov AA, Yalva&ccedil; ME
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0c3820d3041545cc8ceccca1baf08e1d
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spelling	oai:doaj.org-article:0c3820d3041545cc8ceccca1baf08e1d2021-12-02T07:28:37ZDifferentiation of human stem cells is promoted by amphiphilic pluronic block copolymers1176-91141178-2013https://doaj.org/article/0c3820d3041545cc8ceccca1baf08e1d2012-09-01T00:00:00Zhttp://www.dovepress.com/differentiation-of-human-stem-cells-is-promoted-by-amphiphilic-pluroni-a10961https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ayseg&uuml;l Doğan,1 Mehmet E Yalva&ccedil;,1,2 Fikrettin Şahin,1 Alexander V Kabanov,3&ndash;5 Andr&aacute;s Palot&aacute;s,6 Albert A Rizvanov71Department of Genetics and BioEngineering, College of Engineering and Architecture, Yeditepe University, Istanbul, Turkey; 2Center for Gene Therapy, Nationwide Children&#39;s Hospital, Ohio State University, Columbus, OH, USA; 3Center for Drug Delivery and Nanomedicine, 4Department of Pharmaceutical Sciences, College of Pharmacy, Durham Research Center, University of Nebraska Medical Center, Omaha, NE, USA; 5Laboratory of Chemical Design of Bio-nano-materials, Department of Chemistry, Mikhail V Lomonosov Moscow State University, Moscow, Russia; 6Asklepios-Med, Szeged, Hungary; 7Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, RussiaAbstract: Stem cell usage provides novel avenues of tissue regeneration and therapeutics across disciplines. Apart from ethical considerations, the selection and amplification of donor stem cells remain a challenge. Various biopolymers with a wide range of properties have been used extensively to deliver biomolecules such as drugs, growth factors and nucleic acids, as well as to provide biomimetic surface for cellular adhesion. Using human tooth germ stem cells with high proliferation and transformation capacity, we have investigated a range of biopolymers to assess their potential for tissue engineering. Tolerability, toxicity, and their ability to direct differentiation were evaluated. The majority of pluronics, consisting of both hydrophilic and hydrophobic poly(ethylene oxide) chains, either exerted cytotoxicity or had no significant effect on human tooth germ stem cells; whereas F68 increased the multi-potency of stem cells, and efficiently transformed them into osteogenic, chondrogenic, and adipogenic tissues. The data suggest that differentiation and maturation of stem cells can be promoted by selecting the appropriate mechanical and chemical properties of polymers. It has been shown for the first time that F68, with its unique molecular characteristics, has a great potential to increase the differentiation of cells, which may lead to the development of new tissue engineering strategies in regenerative medicine.Keywords: biopolymer, differentiation, human tooth germ stem cell (hTGSC), mesenchymal stem cell, pluronic, toxicityDoğan AŞahin FKabanov AVPalot&aacute;s ARizvanov AAYalva&ccedil; MEDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4849-4860 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Doğan A Şahin F Kabanov AV Palot&aacute;s A Rizvanov AA Yalva&ccedil; ME Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers
description	Ayseg&uuml;l Doğan,1 Mehmet E Yalva&ccedil;,1,2 Fikrettin Şahin,1 Alexander V Kabanov,3&ndash;5 Andr&aacute;s Palot&aacute;s,6 Albert A Rizvanov71Department of Genetics and BioEngineering, College of Engineering and Architecture, Yeditepe University, Istanbul, Turkey; 2Center for Gene Therapy, Nationwide Children&#39;s Hospital, Ohio State University, Columbus, OH, USA; 3Center for Drug Delivery and Nanomedicine, 4Department of Pharmaceutical Sciences, College of Pharmacy, Durham Research Center, University of Nebraska Medical Center, Omaha, NE, USA; 5Laboratory of Chemical Design of Bio-nano-materials, Department of Chemistry, Mikhail V Lomonosov Moscow State University, Moscow, Russia; 6Asklepios-Med, Szeged, Hungary; 7Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, RussiaAbstract: Stem cell usage provides novel avenues of tissue regeneration and therapeutics across disciplines. Apart from ethical considerations, the selection and amplification of donor stem cells remain a challenge. Various biopolymers with a wide range of properties have been used extensively to deliver biomolecules such as drugs, growth factors and nucleic acids, as well as to provide biomimetic surface for cellular adhesion. Using human tooth germ stem cells with high proliferation and transformation capacity, we have investigated a range of biopolymers to assess their potential for tissue engineering. Tolerability, toxicity, and their ability to direct differentiation were evaluated. The majority of pluronics, consisting of both hydrophilic and hydrophobic poly(ethylene oxide) chains, either exerted cytotoxicity or had no significant effect on human tooth germ stem cells; whereas F68 increased the multi-potency of stem cells, and efficiently transformed them into osteogenic, chondrogenic, and adipogenic tissues. The data suggest that differentiation and maturation of stem cells can be promoted by selecting the appropriate mechanical and chemical properties of polymers. It has been shown for the first time that F68, with its unique molecular characteristics, has a great potential to increase the differentiation of cells, which may lead to the development of new tissue engineering strategies in regenerative medicine.Keywords: biopolymer, differentiation, human tooth germ stem cell (hTGSC), mesenchymal stem cell, pluronic, toxicity
format	article
author	Doğan A Şahin F Kabanov AV Palot&aacute;s A Rizvanov AA Yalva&ccedil; ME
author_facet	Doğan A Şahin F Kabanov AV Palot&aacute;s A Rizvanov AA Yalva&ccedil; ME
author_sort	Doğan A
title	Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers
title_short	Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers
title_full	Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers
title_fullStr	Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers
title_full_unstemmed	Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers
title_sort	differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/0c3820d3041545cc8ceccca1baf08e1d
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Differentiation of human stem cells is promoted by amphiphilic pluronic block copolymers

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