The Effect of Feeding Restriction on the Microbiota and Metabolome Response in Late-Phase Laying Hens

This study investigated cecal bacterial community profile, cecal and serum metabolites, and its biosynthesis pathway in late-phase laying hens during 6 weeks feeding restriction (FR), using 16S rDNA as gene sequencing and non-targeted LC-MS/MS as metabolomics approach. We used three groups (ad libit...

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Autores principales:	Clara Ajeng Artdita, Yi-Ru Zhuang, Tzu-Yu Liu, Chih-Yuan Cheng, Felix Shih-Hsiang Hsiao, Yuan-Yu Lin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dietary restriction laying hens serum cecal bacterial profile metabolite profile Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/0c450cc2d4ec435083a5b46d5fbc14e9
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spelling	oai:doaj.org-article:0c450cc2d4ec435083a5b46d5fbc14e92021-11-25T16:14:04ZThe Effect of Feeding Restriction on the Microbiota and Metabolome Response in Late-Phase Laying Hens10.3390/ani111130432076-2615https://doaj.org/article/0c450cc2d4ec435083a5b46d5fbc14e92021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3043https://doaj.org/toc/2076-2615This study investigated cecal bacterial community profile, cecal and serum metabolites, and its biosynthesis pathway in late-phase laying hens during 6 weeks feeding restriction (FR), using 16S rDNA as gene sequencing and non-targeted LC-MS/MS as metabolomics approach. We used three groups (ad libitum, FR20, and FR40). FR can reduce excessive fat in late-phase laying hens, while egg production rate is not affected, except for the FR40 group. In phylum level, FR20 had more population of Bacteriodetes and Firmicutes amongst groups. The same result is at genus level, FR20 were higher of the predominant genus (<i>Bacteroides</i> and <i>Rikenellaceae_RC9_gut_group</i>). Both of FR20 and FR40 reduced Proteobacteria as potential pathogenic bacteria. Non-targeted metabolomic analysis revealed that FR20 modified 20 metabolites in cecal and 10 metabolites in serum of laying hens, whereas 48 cecal metabolites and 31 serum metabolites has revealed in FR40. KEGG assay showed FR20 and FR40 upregulated lipid, carbohydrate, amino acid, nucleic acid pathway, and FR40 modified steroid metabolism in cecal analysis. In serum, only FR40 modified lipid, amino acid pathway, and carbohydrate biosynthesis were shown. This study showed that FR during late-phase laying hens altered the microbiome composition, modified metabolites profile and biosynthesis of the cecal as well as serum.Clara Ajeng ArtditaYi-Ru ZhuangTzu-Yu LiuChih-Yuan ChengFelix Shih-Hsiang HsiaoYuan-Yu LinMDPI AGarticledietary restrictionlaying hensserumcecal bacterial profilemetabolite profileVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3043, p 3043 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dietary restriction laying hens serum cecal bacterial profile metabolite profile Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	dietary restriction laying hens serum cecal bacterial profile metabolite profile Veterinary medicine SF600-1100 Zoology QL1-991 Clara Ajeng Artdita Yi-Ru Zhuang Tzu-Yu Liu Chih-Yuan Cheng Felix Shih-Hsiang Hsiao Yuan-Yu Lin The Effect of Feeding Restriction on the Microbiota and Metabolome Response in Late-Phase Laying Hens
description	This study investigated cecal bacterial community profile, cecal and serum metabolites, and its biosynthesis pathway in late-phase laying hens during 6 weeks feeding restriction (FR), using 16S rDNA as gene sequencing and non-targeted LC-MS/MS as metabolomics approach. We used three groups (ad libitum, FR20, and FR40). FR can reduce excessive fat in late-phase laying hens, while egg production rate is not affected, except for the FR40 group. In phylum level, FR20 had more population of Bacteriodetes and Firmicutes amongst groups. The same result is at genus level, FR20 were higher of the predominant genus (<i>Bacteroides</i> and <i>Rikenellaceae_RC9_gut_group</i>). Both of FR20 and FR40 reduced Proteobacteria as potential pathogenic bacteria. Non-targeted metabolomic analysis revealed that FR20 modified 20 metabolites in cecal and 10 metabolites in serum of laying hens, whereas 48 cecal metabolites and 31 serum metabolites has revealed in FR40. KEGG assay showed FR20 and FR40 upregulated lipid, carbohydrate, amino acid, nucleic acid pathway, and FR40 modified steroid metabolism in cecal analysis. In serum, only FR40 modified lipid, amino acid pathway, and carbohydrate biosynthesis were shown. This study showed that FR during late-phase laying hens altered the microbiome composition, modified metabolites profile and biosynthesis of the cecal as well as serum.
format	article
author	Clara Ajeng Artdita Yi-Ru Zhuang Tzu-Yu Liu Chih-Yuan Cheng Felix Shih-Hsiang Hsiao Yuan-Yu Lin
author_facet	Clara Ajeng Artdita Yi-Ru Zhuang Tzu-Yu Liu Chih-Yuan Cheng Felix Shih-Hsiang Hsiao Yuan-Yu Lin
author_sort	Clara Ajeng Artdita
title	The Effect of Feeding Restriction on the Microbiota and Metabolome Response in Late-Phase Laying Hens
title_short	The Effect of Feeding Restriction on the Microbiota and Metabolome Response in Late-Phase Laying Hens
title_full	The Effect of Feeding Restriction on the Microbiota and Metabolome Response in Late-Phase Laying Hens
title_fullStr	The Effect of Feeding Restriction on the Microbiota and Metabolome Response in Late-Phase Laying Hens
title_full_unstemmed	The Effect of Feeding Restriction on the Microbiota and Metabolome Response in Late-Phase Laying Hens
title_sort	effect of feeding restriction on the microbiota and metabolome response in late-phase laying hens
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0c450cc2d4ec435083a5b46d5fbc14e9
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The Effect of Feeding Restriction on the Microbiota and Metabolome Response in Late-Phase Laying Hens

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