Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome.

Merkel Cell Polyomavirus (MCPyV) genomes are clonally integrated in tumor tissues of approximately 85% of all Merkel cell carcinoma (MCC) cases, a highly aggressive tumor of the skin which predominantly afflicts elderly and immunosuppressed patients. All integrated viral genomes recovered from MCC t...

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Autores principales:	Friederike Neumann, Sophie Borchert, Claudia Schmidt, Rudolph Reimer, Heinrich Hohenberg, Nicole Fischer, Adam Grundhoff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c47849d92c14ff680859362480d2774
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spelling	oai:doaj.org-article:0c47849d92c14ff680859362480d27742021-11-18T07:31:32ZReplication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome.1932-620310.1371/journal.pone.0029112https://doaj.org/article/0c47849d92c14ff680859362480d27742011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22216177/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Merkel Cell Polyomavirus (MCPyV) genomes are clonally integrated in tumor tissues of approximately 85% of all Merkel cell carcinoma (MCC) cases, a highly aggressive tumor of the skin which predominantly afflicts elderly and immunosuppressed patients. All integrated viral genomes recovered from MCC tissue or MCC cell lines harbor signature mutations in the early gene transcript encoding for the large T-Antigen (LT-Ag). These mutations selectively abrogate the ability of LT-Ag to support viral replication while still maintaining its Rb-binding activity, suggesting a continuous requirement for LT-Ag mediated cell cycle deregulation during MCC pathogenesis. To gain a better understanding of MCPyV biology, in vitro MCPyV replication systems are required. We have generated a synthetic MCPyV genomic clone (MCVSyn) based on the consensus sequence of MCC-derived sequences deposited in the NCBI database. Here, we demonstrate that transfection of recircularized MCVSyn DNA into some human cell lines recapitulates efficient replication of the viral genome, early and late gene expression together with virus particle formation. However, serial transmission of infectious virus was not observed. This in vitro culturing system allows the study of viral replication and will facilitate the molecular dissection of important aspects of the MCPyV lifecycle.Friederike NeumannSophie BorchertClaudia SchmidtRudolph ReimerHeinrich HohenbergNicole FischerAdam GrundhoffPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e29112 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Friederike Neumann Sophie Borchert Claudia Schmidt Rudolph Reimer Heinrich Hohenberg Nicole Fischer Adam Grundhoff Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome.
description	Merkel Cell Polyomavirus (MCPyV) genomes are clonally integrated in tumor tissues of approximately 85% of all Merkel cell carcinoma (MCC) cases, a highly aggressive tumor of the skin which predominantly afflicts elderly and immunosuppressed patients. All integrated viral genomes recovered from MCC tissue or MCC cell lines harbor signature mutations in the early gene transcript encoding for the large T-Antigen (LT-Ag). These mutations selectively abrogate the ability of LT-Ag to support viral replication while still maintaining its Rb-binding activity, suggesting a continuous requirement for LT-Ag mediated cell cycle deregulation during MCC pathogenesis. To gain a better understanding of MCPyV biology, in vitro MCPyV replication systems are required. We have generated a synthetic MCPyV genomic clone (MCVSyn) based on the consensus sequence of MCC-derived sequences deposited in the NCBI database. Here, we demonstrate that transfection of recircularized MCVSyn DNA into some human cell lines recapitulates efficient replication of the viral genome, early and late gene expression together with virus particle formation. However, serial transmission of infectious virus was not observed. This in vitro culturing system allows the study of viral replication and will facilitate the molecular dissection of important aspects of the MCPyV lifecycle.
format	article
author	Friederike Neumann Sophie Borchert Claudia Schmidt Rudolph Reimer Heinrich Hohenberg Nicole Fischer Adam Grundhoff
author_facet	Friederike Neumann Sophie Borchert Claudia Schmidt Rudolph Reimer Heinrich Hohenberg Nicole Fischer Adam Grundhoff
author_sort	Friederike Neumann
title	Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome.
title_short	Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome.
title_full	Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome.
title_fullStr	Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome.
title_full_unstemmed	Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome.
title_sort	replication, gene expression and particle production by a consensus merkel cell polyomavirus (mcpyv) genome.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/0c47849d92c14ff680859362480d2774
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Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome.

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