Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis

Abstract Cobalamin (vitamin B12) is a structurally complex molecule that acts as a cofactor for enzymes and regulates gene expression through so-called riboswitches. The existing literature on the vitamin B12 synthesis capacity in Mycobacterium tuberculosis is ambiguous, while in non-tuberculous myc...

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Autores principales:	Alina Minias, Filip Gąsior, Anna Brzostek, Tomasz Jagielski, Jarosław Dziadek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c4eaab9d12343ddbd3253ad0c70cd11
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spelling	oai:doaj.org-article:0c4eaab9d12343ddbd3253ad0c70cd112021-12-02T17:47:15ZCobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis10.1038/s41598-021-91430-w2045-2322https://doaj.org/article/0c4eaab9d12343ddbd3253ad0c70cd112021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91430-whttps://doaj.org/toc/2045-2322Abstract Cobalamin (vitamin B12) is a structurally complex molecule that acts as a cofactor for enzymes and regulates gene expression through so-called riboswitches. The existing literature on the vitamin B12 synthesis capacity in Mycobacterium tuberculosis is ambiguous, while in non-tuberculous mycobacteria (NTM) is rather marginal. Here we present the results of our investigation into the occurrence of vitamin B12 in mycobacteria. For detection purposes, immunoassay methods were applied to cell lysates of NTM and M. tuberculosis clinical and laboratory strains grown under different conditions. We show that whereas vitamin B12 is present in cells of various NTM species, it cannot be evidenced in strains of differently cultured M. tuberculosis, even though the genes responsible for vitamin B12 synthesis are actively expressed based on RNA-Seq data. In summary, we conclude that the production of vitamin B12 does occur in mycobacteria, with the likely exception of M. tuberculosis. Our results provide direct evidence of vitamin B12 synthesis in a clinically important group of bacteria.Alina MiniasFilip GąsiorAnna BrzostekTomasz JagielskiJarosław DziadekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alina Minias Filip Gąsior Anna Brzostek Tomasz Jagielski Jarosław Dziadek Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
description	Abstract Cobalamin (vitamin B12) is a structurally complex molecule that acts as a cofactor for enzymes and regulates gene expression through so-called riboswitches. The existing literature on the vitamin B12 synthesis capacity in Mycobacterium tuberculosis is ambiguous, while in non-tuberculous mycobacteria (NTM) is rather marginal. Here we present the results of our investigation into the occurrence of vitamin B12 in mycobacteria. For detection purposes, immunoassay methods were applied to cell lysates of NTM and M. tuberculosis clinical and laboratory strains grown under different conditions. We show that whereas vitamin B12 is present in cells of various NTM species, it cannot be evidenced in strains of differently cultured M. tuberculosis, even though the genes responsible for vitamin B12 synthesis are actively expressed based on RNA-Seq data. In summary, we conclude that the production of vitamin B12 does occur in mycobacteria, with the likely exception of M. tuberculosis. Our results provide direct evidence of vitamin B12 synthesis in a clinically important group of bacteria.
format	article
author	Alina Minias Filip Gąsior Anna Brzostek Tomasz Jagielski Jarosław Dziadek
author_facet	Alina Minias Filip Gąsior Anna Brzostek Tomasz Jagielski Jarosław Dziadek
author_sort	Alina Minias
title	Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
title_short	Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
title_full	Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
title_fullStr	Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
title_full_unstemmed	Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
title_sort	cobalamin is present in cells of non-tuberculous mycobacteria, but not in mycobacterium tuberculosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0c4eaab9d12343ddbd3253ad0c70cd11
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Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis

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