Anti-asthmatic effects of Phlomis umbrosa Turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis

Background: Phlomis umbrosa Turczaninow has been used as a tradition herbal medicine for treating various inflammatory diseases. Purpose: In present study, we explored the effects of P. umbrosa on asthma induced by ovalbumin (OVA) and elucidated the mechanism via in vivo verification and network pha...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	So-Won Pak, A Yeong Lee, Yun-Soo Seo, Se-Jin Lee, Woong-Il Kim, Dong-Ho Shin, Jong-Choon Kim, Joong-Sun Kim, Je-Oh Lim, In-Sik Shin
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Phlomis umbrosa Turczaninow Asthma Network analysis ERK MMP-9 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0c4ef0ca0e414de2926dbd02214af3e9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0c4ef0ca0e414de2926dbd02214af3e9
record_format	dspace
spelling	oai:doaj.org-article:0c4ef0ca0e414de2926dbd02214af3e92021-11-12T04:25:38ZAnti-asthmatic effects of Phlomis umbrosa Turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis0753-332210.1016/j.biopha.2021.112410https://doaj.org/article/0c4ef0ca0e414de2926dbd02214af3e92022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011963https://doaj.org/toc/0753-3322Background: Phlomis umbrosa Turczaninow has been used as a tradition herbal medicine for treating various inflammatory diseases. Purpose: In present study, we explored the effects of P. umbrosa on asthma induced by ovalbumin (OVA) and elucidated the mechanism via in vivo verification and network pharmacology prediction. Methods: The animals were intraperitoneally injected OVA on day 1 and 14, followed by OVA inhalation on days 21, 22, and 23. The animals were daily treated P. umbrosa extract (PUE, 20 and 40 mg/kg) by oral gavage from day 18 to day 23. Results: PUE significantly decreased airway hyperresponsiveness, eosinophilia, and the production of inflammatory cytokines and OVA specific immunoglobulin E in animals with asthma, along with a reduction in airway inflammation and mucus secretion in lung tissue. In network analysis, antiasthmatic effects of PUE were closely related with suppression of mitogen-activated protein kinases and matrix metalloproteinases (MMPs). Consistent with the results from network analysis, PUE suppressed the phosphorylation of ERK and p65, which was accompanied by a decline in MMP-9 expression. Conclusion: Administration of PUE effectively reduced allergic responses in asthmatic mice, which was associated with the suppressed phosphorylation of ERK and p65, and expression of MMP-9. These results indicate that PUE has therapeutic potential to treat allergic asthma.So-Won PakA Yeong LeeYun-Soo SeoSe-Jin LeeWoong-Il KimDong-Ho ShinJong-Choon KimJoong-Sun KimJe-Oh LimIn-Sik ShinElsevierarticlePhlomis umbrosa TurczaninowAsthmaNetwork analysisERKMMP-9Therapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112410- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Phlomis umbrosa Turczaninow Asthma Network analysis ERK MMP-9 Therapeutics. Pharmacology RM1-950
spellingShingle	Phlomis umbrosa Turczaninow Asthma Network analysis ERK MMP-9 Therapeutics. Pharmacology RM1-950 So-Won Pak A Yeong Lee Yun-Soo Seo Se-Jin Lee Woong-Il Kim Dong-Ho Shin Jong-Choon Kim Joong-Sun Kim Je-Oh Lim In-Sik Shin Anti-asthmatic effects of Phlomis umbrosa Turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis
description	Background: Phlomis umbrosa Turczaninow has been used as a tradition herbal medicine for treating various inflammatory diseases. Purpose: In present study, we explored the effects of P. umbrosa on asthma induced by ovalbumin (OVA) and elucidated the mechanism via in vivo verification and network pharmacology prediction. Methods: The animals were intraperitoneally injected OVA on day 1 and 14, followed by OVA inhalation on days 21, 22, and 23. The animals were daily treated P. umbrosa extract (PUE, 20 and 40 mg/kg) by oral gavage from day 18 to day 23. Results: PUE significantly decreased airway hyperresponsiveness, eosinophilia, and the production of inflammatory cytokines and OVA specific immunoglobulin E in animals with asthma, along with a reduction in airway inflammation and mucus secretion in lung tissue. In network analysis, antiasthmatic effects of PUE were closely related with suppression of mitogen-activated protein kinases and matrix metalloproteinases (MMPs). Consistent with the results from network analysis, PUE suppressed the phosphorylation of ERK and p65, which was accompanied by a decline in MMP-9 expression. Conclusion: Administration of PUE effectively reduced allergic responses in asthmatic mice, which was associated with the suppressed phosphorylation of ERK and p65, and expression of MMP-9. These results indicate that PUE has therapeutic potential to treat allergic asthma.
format	article
author	So-Won Pak A Yeong Lee Yun-Soo Seo Se-Jin Lee Woong-Il Kim Dong-Ho Shin Jong-Choon Kim Joong-Sun Kim Je-Oh Lim In-Sik Shin
author_facet	So-Won Pak A Yeong Lee Yun-Soo Seo Se-Jin Lee Woong-Il Kim Dong-Ho Shin Jong-Choon Kim Joong-Sun Kim Je-Oh Lim In-Sik Shin
author_sort	So-Won Pak
title	Anti-asthmatic effects of Phlomis umbrosa Turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis
title_short	Anti-asthmatic effects of Phlomis umbrosa Turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis
title_full	Anti-asthmatic effects of Phlomis umbrosa Turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis
title_fullStr	Anti-asthmatic effects of Phlomis umbrosa Turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis
title_full_unstemmed	Anti-asthmatic effects of Phlomis umbrosa Turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis
title_sort	anti-asthmatic effects of phlomis umbrosa turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/0c4ef0ca0e414de2926dbd02214af3e9
work_keys_str_mv	AT sowonpak antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis AT ayeonglee antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis AT yunsooseo antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis AT sejinlee antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis AT woongilkim antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis AT donghoshin antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis AT jongchoonkim antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis AT joongsunkim antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis AT jeohlim antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis AT insikshin antiasthmaticeffectsofphlomisumbrosaturczaninowusingovalbumininducedasthmamurinemodelandnetworkpharmacologyanalysis
_version_	1718431271868170240

Anti-asthmatic effects of Phlomis umbrosa Turczaninow using ovalbumin induced asthma murine model and network pharmacology analysis

Ejemplares similares