Comparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).

We present the first comparison of global transcriptional changes in canine and human diffuse large B-cell lymphoma (DLBCL), with particular reference to the nuclear factor-kappa B (NF-κB) pathway. Microarray data generated from canine DLBCL and normal lymph nodes were used for differential expressi...

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Autores principales:	Manikhandan A V Mudaliar, Ross D Haggart, Gino Miele, Grant Sellar, Karen A L Tan, John R Goodlad, Elspeth Milne, David M Vail, Ilene Kurzman, Daniel Crowther, David J Argyle
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c58f125718442bcbbdb8d21cbf07eb7
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spelling	oai:doaj.org-article:0c58f125718442bcbbdb8d21cbf07eb72021-11-18T08:57:02ZComparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).1932-620310.1371/journal.pone.0072591https://doaj.org/article/0c58f125718442bcbbdb8d21cbf07eb72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24023754/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We present the first comparison of global transcriptional changes in canine and human diffuse large B-cell lymphoma (DLBCL), with particular reference to the nuclear factor-kappa B (NF-κB) pathway. Microarray data generated from canine DLBCL and normal lymph nodes were used for differential expression, co-expression and pathway analyses, and compared with analysis of microarray data from human healthy and DLBCL lymph nodes. The comparisons at gene level were performed by mapping the probesets in canine microarrays to orthologous genes in humans and vice versa. A considerable number of differentially expressed genes between canine lymphoma and healthy lymph node samples were also found differentially expressed between human DLBCL and healthy lymph node samples. Principal component analysis using a literature-derived NF-κB target gene set mapped to orthologous canine array probesets and human array probesets clearly separated the healthy and cancer samples in both datasets. The analysis demonstrated that for both human and canine DLBCL there is activation of the NF-κB/p65 canonical pathway, indicating that canine lymphoma could be used as a model to study NF-κB-targeted therapeutics for human lymphoma. To validate this, tissue arrays were generated for canine and human NHL and immunohistochemistry was employed to assess NF-κB activation status. In addition, human and canine B-cell lymphoma lines were assessed for NF-κB activity and the effects of NF-κB inhibition.Manikhandan A V MudaliarRoss D HaggartGino MieleGrant SellarKaren A L TanJohn R GoodladElspeth MilneDavid M VailIlene KurzmanDaniel CrowtherDavid J ArgylePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e72591 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manikhandan A V Mudaliar Ross D Haggart Gino Miele Grant Sellar Karen A L Tan John R Goodlad Elspeth Milne David M Vail Ilene Kurzman Daniel Crowther David J Argyle Comparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).
description	We present the first comparison of global transcriptional changes in canine and human diffuse large B-cell lymphoma (DLBCL), with particular reference to the nuclear factor-kappa B (NF-κB) pathway. Microarray data generated from canine DLBCL and normal lymph nodes were used for differential expression, co-expression and pathway analyses, and compared with analysis of microarray data from human healthy and DLBCL lymph nodes. The comparisons at gene level were performed by mapping the probesets in canine microarrays to orthologous genes in humans and vice versa. A considerable number of differentially expressed genes between canine lymphoma and healthy lymph node samples were also found differentially expressed between human DLBCL and healthy lymph node samples. Principal component analysis using a literature-derived NF-κB target gene set mapped to orthologous canine array probesets and human array probesets clearly separated the healthy and cancer samples in both datasets. The analysis demonstrated that for both human and canine DLBCL there is activation of the NF-κB/p65 canonical pathway, indicating that canine lymphoma could be used as a model to study NF-κB-targeted therapeutics for human lymphoma. To validate this, tissue arrays were generated for canine and human NHL and immunohistochemistry was employed to assess NF-κB activation status. In addition, human and canine B-cell lymphoma lines were assessed for NF-κB activity and the effects of NF-κB inhibition.
format	article
author	Manikhandan A V Mudaliar Ross D Haggart Gino Miele Grant Sellar Karen A L Tan John R Goodlad Elspeth Milne David M Vail Ilene Kurzman Daniel Crowther David J Argyle
author_facet	Manikhandan A V Mudaliar Ross D Haggart Gino Miele Grant Sellar Karen A L Tan John R Goodlad Elspeth Milne David M Vail Ilene Kurzman Daniel Crowther David J Argyle
author_sort	Manikhandan A V Mudaliar
title	Comparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).
title_short	Comparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).
title_full	Comparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).
title_fullStr	Comparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).
title_full_unstemmed	Comparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).
title_sort	comparative gene expression profiling identifies common molecular signatures of nf-κb activation in canine and human diffuse large b cell lymphoma (dlbcl).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0c58f125718442bcbbdb8d21cbf07eb7
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Comparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).

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