Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species

Abstract This study investigated the biochemical properties, enzyme activities, isoenzyme pattern, and molecular weight of three types of digestive enzyme from six freshwater fish species: Puntius gonionotus (common silver barb), Puntioplites proctozysron (Smith’s barb), Oreochromis niloticus (Nile...

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Autores principales:	Chamaiporn Champasri, Suthathip Phetlum, Chanakan Pornchoo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c59572d7340458da762a29257fdb17e
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spelling	oai:doaj.org-article:0c59572d7340458da762a29257fdb17e2021-12-02T13:20:02ZDiverse activities and biochemical properties of amylase and proteases from six freshwater fish species10.1038/s41598-021-85258-72045-2322https://doaj.org/article/0c59572d7340458da762a29257fdb17e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85258-7https://doaj.org/toc/2045-2322Abstract This study investigated the biochemical properties, enzyme activities, isoenzyme pattern, and molecular weight of three types of digestive enzyme from six freshwater fish species: Puntius gonionotus (common silver barb), Puntioplites proctozysron (Smith’s barb), Oreochromis niloticus (Nile tilapia), Hemibagrus spilopterus (yellow mystus), Ompok bimaculatus (butter catfish), and Kryptopterus geminus (sheatfish). The optimum pHs for amylase and alkaline protease activities were 7.0–8.0 and 8.0–10.0, and the optimum temperatures were 45–60 °C and 50–55 °C, respectively. A pepsin-like enzyme was detected in all three carnivorous fishes (Ompok bimaculatus, Kryptopterus geminus, and Hemibagrus spilopterus) with optimum reaction pH of 2.0 for each and optimum reaction temperatures 50–55 °C. In optimum reaction conditions, the amylase and alkaline protease from Puntioplites proctozyron showed the highest activities. Lower activities of all enzymes were observed at temperature (29 °C) of Lam Nam Choen swamp than at the optimum reaction temperatures. The fish species contained one to three and five to eight isoforms of amylase and alkaline protease, respectively, with molecular weights from 19.5 to 175 kDa. Both the alkaline proteases and amylases were stable in wide pH and temperature ranges.Chamaiporn ChampasriSuthathip PhetlumChanakan PornchooNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chamaiporn Champasri Suthathip Phetlum Chanakan Pornchoo Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species
description	Abstract This study investigated the biochemical properties, enzyme activities, isoenzyme pattern, and molecular weight of three types of digestive enzyme from six freshwater fish species: Puntius gonionotus (common silver barb), Puntioplites proctozysron (Smith’s barb), Oreochromis niloticus (Nile tilapia), Hemibagrus spilopterus (yellow mystus), Ompok bimaculatus (butter catfish), and Kryptopterus geminus (sheatfish). The optimum pHs for amylase and alkaline protease activities were 7.0–8.0 and 8.0–10.0, and the optimum temperatures were 45–60 °C and 50–55 °C, respectively. A pepsin-like enzyme was detected in all three carnivorous fishes (Ompok bimaculatus, Kryptopterus geminus, and Hemibagrus spilopterus) with optimum reaction pH of 2.0 for each and optimum reaction temperatures 50–55 °C. In optimum reaction conditions, the amylase and alkaline protease from Puntioplites proctozyron showed the highest activities. Lower activities of all enzymes were observed at temperature (29 °C) of Lam Nam Choen swamp than at the optimum reaction temperatures. The fish species contained one to three and five to eight isoforms of amylase and alkaline protease, respectively, with molecular weights from 19.5 to 175 kDa. Both the alkaline proteases and amylases were stable in wide pH and temperature ranges.
format	article
author	Chamaiporn Champasri Suthathip Phetlum Chanakan Pornchoo
author_facet	Chamaiporn Champasri Suthathip Phetlum Chanakan Pornchoo
author_sort	Chamaiporn Champasri
title	Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species
title_short	Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species
title_full	Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species
title_fullStr	Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species
title_full_unstemmed	Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species
title_sort	diverse activities and biochemical properties of amylase and proteases from six freshwater fish species
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0c59572d7340458da762a29257fdb17e
work_keys_str_mv	AT chamaipornchampasri diverseactivitiesandbiochemicalpropertiesofamylaseandproteasesfromsixfreshwaterfishspecies AT suthathipphetlum diverseactivitiesandbiochemicalpropertiesofamylaseandproteasesfromsixfreshwaterfishspecies AT chanakanpornchoo diverseactivitiesandbiochemicalpropertiesofamylaseandproteasesfromsixfreshwaterfishspecies
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Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species

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