Use of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples

The use of direct PCR technique without DNA extraction in the confirmation test for Salmonella typhimurium ATCC 14028 bacteria on meatball samples was carried out in the Food and Drug molecular biology testing laboratory Administration in Gorontalo. The basis of this research is to have an impact on...

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Autores principales:	Alfi Sophian, Ratna Purwaningsih, Muindar Muindar, Eka Putri Juniarti Igirisa, Muhammad Luthfi Amirullah
Formato:	article
Lenguaje:	EN ID
Publicado:	Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya 2021
Materias:	Direct DNA PCR Salmonella Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/0c66a027bc84444bacc6899d9a472733
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spelling	oai:doaj.org-article:0c66a027bc84444bacc6899d9a4727332021-12-01T04:44:50ZUse of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples10.33084/bjop.v4i4.21872621-4814https://doaj.org/article/0c66a027bc84444bacc6899d9a4727332021-11-01T00:00:00Zhttp://journal.umpalangkaraya.ac.id/index.php/bjop/article/view/2187https://doaj.org/toc/2621-4814The use of direct PCR technique without DNA extraction in the confirmation test for Salmonella typhimurium ATCC 14028 bacteria on meatball samples was carried out in the Food and Drug molecular biology testing laboratory Administration in Gorontalo. The basis of this research is to have an impact on economic value in carrying out the confirmation test for S. typhimurium ATCC 14028, where testing is carried out conventionally, namely DNA extraction, which requires a large amount of money. Hence, it is necessary to innovate to modify the testing phase so that it is more effective and efficient. The purpose of this study was to see whether the direct PCR technique without DNA extraction can be done for the confirmation test of S. typhimurium ATCC 14028 on meatball samples. This study's sample consisted of 20 types of meatball samples spiked with S. typhimurium ATCC 14028 cultures. The method used in this study was qPCR analysis using the SYBR Green method. Data analysis was carried out based on 2 main criteria: (1) Ct analysis and (2) Tm analysis. Real-time PCR analysis results obtained Ct values in the range 14.14 - 15.20 with an average of 14.82 and Tm values 85.20 - 86.30 with an average of 85.79. Based on these data, it can be concluded that using direct PCR can be used for testing confirmation of S. typhimurium ATCC 14028 on meatball samples. Alfi SophianRatna PurwaningsihMuindar MuindarEka Putri Juniarti IgirisaMuhammad Luthfi AmirullahInstitute for Researches and Community Services Universitas Muhammadiyah Palangkaraya articleDirectDNAPCRSalmonellaPharmacy and materia medicaRS1-441ENIDBorneo Journal of Pharmacy, Vol 4, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	Direct DNA PCR Salmonella Pharmacy and materia medica RS1-441
spellingShingle	Direct DNA PCR Salmonella Pharmacy and materia medica RS1-441 Alfi Sophian Ratna Purwaningsih Muindar Muindar Eka Putri Juniarti Igirisa Muhammad Luthfi Amirullah Use of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples
description	The use of direct PCR technique without DNA extraction in the confirmation test for Salmonella typhimurium ATCC 14028 bacteria on meatball samples was carried out in the Food and Drug molecular biology testing laboratory Administration in Gorontalo. The basis of this research is to have an impact on economic value in carrying out the confirmation test for S. typhimurium ATCC 14028, where testing is carried out conventionally, namely DNA extraction, which requires a large amount of money. Hence, it is necessary to innovate to modify the testing phase so that it is more effective and efficient. The purpose of this study was to see whether the direct PCR technique without DNA extraction can be done for the confirmation test of S. typhimurium ATCC 14028 on meatball samples. This study's sample consisted of 20 types of meatball samples spiked with S. typhimurium ATCC 14028 cultures. The method used in this study was qPCR analysis using the SYBR Green method. Data analysis was carried out based on 2 main criteria: (1) Ct analysis and (2) Tm analysis. Real-time PCR analysis results obtained Ct values in the range 14.14 - 15.20 with an average of 14.82 and Tm values 85.20 - 86.30 with an average of 85.79. Based on these data, it can be concluded that using direct PCR can be used for testing confirmation of S. typhimurium ATCC 14028 on meatball samples.
format	article
author	Alfi Sophian Ratna Purwaningsih Muindar Muindar Eka Putri Juniarti Igirisa Muhammad Luthfi Amirullah
author_facet	Alfi Sophian Ratna Purwaningsih Muindar Muindar Eka Putri Juniarti Igirisa Muhammad Luthfi Amirullah
author_sort	Alfi Sophian
title	Use of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples
title_short	Use of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples
title_full	Use of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples
title_fullStr	Use of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples
title_full_unstemmed	Use of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples
title_sort	use of direct pcr technique without dna extraction in confirmation test for salmonella typhimurium bacteria on meatball samples
publisher	Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya
publishDate	2021
url	https://doaj.org/article/0c66a027bc84444bacc6899d9a472733
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Use of Direct PCR Technique Without DNA Extraction in Confirmation Test for Salmonella typhimurium Bacteria on Meatball Samples

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