NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells

NK2 homeobox 1 (NKX2-1) is a thyroid transcription factor essential for proper thyroid formation and maintaining its physiological function. In thyroid cancer, NKX2-1 expression decreases in parallel with declined differentiation. However, the molecular pathways and mechanisms connecting NKX2-1 to t...

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Autores principales:	Yuko Ito, Fumihiko Furuya, Katsumi Taki, Hideaki Suzuki, Hiroki Shimura
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c6dd759a1584754b8767fff4bc3d876
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spelling	oai:doaj.org-article:0c6dd759a1584754b8767fff4bc3d8762021-11-18T06:34:27ZNKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells1932-6203https://doaj.org/article/0c6dd759a1584754b8767fff4bc3d8762021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575255/?tool=EBIhttps://doaj.org/toc/1932-6203NK2 homeobox 1 (NKX2-1) is a thyroid transcription factor essential for proper thyroid formation and maintaining its physiological function. In thyroid cancer, NKX2-1 expression decreases in parallel with declined differentiation. However, the molecular pathways and mechanisms connecting NKX2-1 to thyroid cancer phenotypes are largely unknown. This study aimed to examine the effects of NKX2-1 re-expression on dedifferentiated thyroid cancer cell death and explore the underlying mechanisms. A human papillary thyroid carcinoma cell line lacking NKX2-1 expression was infected with an adenoviral vector containing Nkx2-1. Cell viability decreased after Nkx2-1 transduction and apoptosis and necrosis were detected. Arginase 2 (ARG2), regulator of G protein signaling 4 (RGS4), and RGS5 mRNA expression was greatly increased in Nkx2-1-transducted cells. After suppressing these genes by siRNA, cell death, apoptosis, and necrosis decreased in RGS4 knockdown cells. These findings demonstrated that cell death was induced via apoptosis and necrosis by NKX2-1 re-expression and involves RGS4.Yuko ItoFumihiko FuruyaKatsumi TakiHideaki SuzukiHiroki ShimuraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuko Ito Fumihiko Furuya Katsumi Taki Hideaki Suzuki Hiroki Shimura NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells
description	NK2 homeobox 1 (NKX2-1) is a thyroid transcription factor essential for proper thyroid formation and maintaining its physiological function. In thyroid cancer, NKX2-1 expression decreases in parallel with declined differentiation. However, the molecular pathways and mechanisms connecting NKX2-1 to thyroid cancer phenotypes are largely unknown. This study aimed to examine the effects of NKX2-1 re-expression on dedifferentiated thyroid cancer cell death and explore the underlying mechanisms. A human papillary thyroid carcinoma cell line lacking NKX2-1 expression was infected with an adenoviral vector containing Nkx2-1. Cell viability decreased after Nkx2-1 transduction and apoptosis and necrosis were detected. Arginase 2 (ARG2), regulator of G protein signaling 4 (RGS4), and RGS5 mRNA expression was greatly increased in Nkx2-1-transducted cells. After suppressing these genes by siRNA, cell death, apoptosis, and necrosis decreased in RGS4 knockdown cells. These findings demonstrated that cell death was induced via apoptosis and necrosis by NKX2-1 re-expression and involves RGS4.
format	article
author	Yuko Ito Fumihiko Furuya Katsumi Taki Hideaki Suzuki Hiroki Shimura
author_facet	Yuko Ito Fumihiko Furuya Katsumi Taki Hideaki Suzuki Hiroki Shimura
author_sort	Yuko Ito
title	NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells
title_short	NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells
title_full	NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells
title_fullStr	NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells
title_full_unstemmed	NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells
title_sort	nkx2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0c6dd759a1584754b8767fff4bc3d876
work_keys_str_mv	AT yukoito nkx21reexpressioninducescelldeaththroughapoptosisandnecrosisindedifferentiatedthyroidcarcinomacells AT fumihikofuruya nkx21reexpressioninducescelldeaththroughapoptosisandnecrosisindedifferentiatedthyroidcarcinomacells AT katsumitaki nkx21reexpressioninducescelldeaththroughapoptosisandnecrosisindedifferentiatedthyroidcarcinomacells AT hideakisuzuki nkx21reexpressioninducescelldeaththroughapoptosisandnecrosisindedifferentiatedthyroidcarcinomacells AT hirokishimura nkx21reexpressioninducescelldeaththroughapoptosisandnecrosisindedifferentiatedthyroidcarcinomacells
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NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells

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