Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection

Abstract The Arabidopsis thaliana genome encodes several genes that are known or predicted to participate in the formation of stress granules (SG). One family of genes encodes for Ras GTPase-activating protein–binding protein (G3BP)-like proteins. Seven genes were identified, of which one of the mem...

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Autores principales:	Hendrik Reuper, Khalid Amari, Björn Krenz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c6e2f6335fa4a799ed8c0e6f384f155
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spelling	oai:doaj.org-article:0c6e2f6335fa4a799ed8c0e6f384f1552021-12-02T10:47:54ZAnalyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection10.1038/s41598-021-81276-72045-2322https://doaj.org/article/0c6e2f6335fa4a799ed8c0e6f384f1552021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81276-7https://doaj.org/toc/2045-2322Abstract The Arabidopsis thaliana genome encodes several genes that are known or predicted to participate in the formation of stress granules (SG). One family of genes encodes for Ras GTPase-activating protein–binding protein (G3BP)-like proteins. Seven genes were identified, of which one of the members was already shown to interact with plant virus proteins in a previous study. A phylogenetic and tissue-specific expression analysis, including laser-dissected phloem, by qRT-PCRs was performed and the sub-cellular localization of individual AtG3BP::EYFP fluorescent fusion proteins expressed in Nicotiana benthamiana epidermal cells was observed. Individual AtG3BP-protein interactions in planta were studied using the bimolecular fluorescence complementation approach in combination with confocal imaging in living cells. In addition, the early and late induction of G3BP-like expression upon Turnip mosaic virus infection was investigated by RNAseq and qRT-PCR. The results showed a high divergence of transcription frequency in the different plant tissues, promiscuous protein–protein interaction within the G3BP-like gene family, and a general induction by a viral infection with TuMV in A. thaliana. The information gained from these studies leads to a better understanding of stress granules, in particular their molecular mode of action in the plant and their role in plant virus infection.Hendrik ReuperKhalid AmariBjörn KrenzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hendrik Reuper Khalid Amari Björn Krenz Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection
description	Abstract The Arabidopsis thaliana genome encodes several genes that are known or predicted to participate in the formation of stress granules (SG). One family of genes encodes for Ras GTPase-activating protein–binding protein (G3BP)-like proteins. Seven genes were identified, of which one of the members was already shown to interact with plant virus proteins in a previous study. A phylogenetic and tissue-specific expression analysis, including laser-dissected phloem, by qRT-PCRs was performed and the sub-cellular localization of individual AtG3BP::EYFP fluorescent fusion proteins expressed in Nicotiana benthamiana epidermal cells was observed. Individual AtG3BP-protein interactions in planta were studied using the bimolecular fluorescence complementation approach in combination with confocal imaging in living cells. In addition, the early and late induction of G3BP-like expression upon Turnip mosaic virus infection was investigated by RNAseq and qRT-PCR. The results showed a high divergence of transcription frequency in the different plant tissues, promiscuous protein–protein interaction within the G3BP-like gene family, and a general induction by a viral infection with TuMV in A. thaliana. The information gained from these studies leads to a better understanding of stress granules, in particular their molecular mode of action in the plant and their role in plant virus infection.
format	article
author	Hendrik Reuper Khalid Amari Björn Krenz
author_facet	Hendrik Reuper Khalid Amari Björn Krenz
author_sort	Hendrik Reuper
title	Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection
title_short	Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection
title_full	Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection
title_fullStr	Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection
title_full_unstemmed	Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection
title_sort	analyzing the g3bp-like gene family of arabidopsis thaliana in early turnip mosaic virus infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0c6e2f6335fa4a799ed8c0e6f384f155
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Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection

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