EDTA-enhanced Cr detoxification and its potential toxicity in rice (Oryza sativa L.)

Ethylenediamine tetraacetic acid (EDTA) is a chelating agent used worldwide for the phytoextraction of heavy metals using hyperaccumulating plants as well as in household and industrial purposes and persists in cultivated land for a long time without any known impact on nonhyperaccumulating plants....

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Autores principales:	A. K. M. Nazmul Huda, Muktar Hossain, Rumana Haque Mukta, Mossammad Rima Khatun, Md. Anwarul Haque
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Chromium Phytochelatin Metallothionein ROS Vacuolar sequestration Plant ecology QK900-989
Acceso en línea:	https://doaj.org/article/0c7013adfd5145cbb9432fae8c6ca9b9
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spelling	oai:doaj.org-article:0c7013adfd5145cbb9432fae8c6ca9b92021-12-04T04:36:32ZEDTA-enhanced Cr detoxification and its potential toxicity in rice (Oryza sativa L.)2667-064X10.1016/j.stress.2021.100014https://doaj.org/article/0c7013adfd5145cbb9432fae8c6ca9b92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667064X21000130https://doaj.org/toc/2667-064XEthylenediamine tetraacetic acid (EDTA) is a chelating agent used worldwide for the phytoextraction of heavy metals using hyperaccumulating plants as well as in household and industrial purposes and persists in cultivated land for a long time without any known impact on nonhyperaccumulating plants. In this study, the effects of EDTA in the presence and absence of chromium on the hydroponic solution were evaluated at the cellular and molecular levels in nonhyperaccumulating rice plants (Oryza sativa L.). EDTA application enhanced phytochelatin (PC) and metallothionein (MT) synthesis, which was confirmed by the upregulation of phytochelatin and metallothionein genes in rice roots. However, a lower quantity of EDTA (25 µM) along with chromium enhanced chromium uptake (22.5%) in the root but inhibited its translocation to the shoot, indicating vacuolar sequestration of excessive chromium in the root. Conversely, in the absence of chromium, the H2O2 concentration was found to be increased, which significantly enhanced electrolyte leakage (98.5%) and lipid peroxidation (33.3%) compared with control plants. Our data indicate that excessive PCs and MTs without vacuolar sequestration enhance reactive oxygen species (ROS) generation. The findings of this investigation show that enhanced PCs and MTs owing to EDTA sequester excessive chromium in root vacuoles but, in the absence of sequestration due to a lack of chromium, enhance reactive oxygen species generation, resulting in toxicity. The outcomes of this investigation will open a new avenue of research in phytoextraction or phytoremediation activities.A. K. M. Nazmul HudaMuktar HossainRumana Haque MuktaMossammad Rima KhatunMd. Anwarul HaqueElsevierarticleChromiumPhytochelatinMetallothioneinROSVacuolar sequestrationPlant ecologyQK900-989ENPlant Stress, Vol 2, Iss , Pp 100014- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chromium Phytochelatin Metallothionein ROS Vacuolar sequestration Plant ecology QK900-989
spellingShingle	Chromium Phytochelatin Metallothionein ROS Vacuolar sequestration Plant ecology QK900-989 A. K. M. Nazmul Huda Muktar Hossain Rumana Haque Mukta Mossammad Rima Khatun Md. Anwarul Haque EDTA-enhanced Cr detoxification and its potential toxicity in rice (Oryza sativa L.)
description	Ethylenediamine tetraacetic acid (EDTA) is a chelating agent used worldwide for the phytoextraction of heavy metals using hyperaccumulating plants as well as in household and industrial purposes and persists in cultivated land for a long time without any known impact on nonhyperaccumulating plants. In this study, the effects of EDTA in the presence and absence of chromium on the hydroponic solution were evaluated at the cellular and molecular levels in nonhyperaccumulating rice plants (Oryza sativa L.). EDTA application enhanced phytochelatin (PC) and metallothionein (MT) synthesis, which was confirmed by the upregulation of phytochelatin and metallothionein genes in rice roots. However, a lower quantity of EDTA (25 µM) along with chromium enhanced chromium uptake (22.5%) in the root but inhibited its translocation to the shoot, indicating vacuolar sequestration of excessive chromium in the root. Conversely, in the absence of chromium, the H2O2 concentration was found to be increased, which significantly enhanced electrolyte leakage (98.5%) and lipid peroxidation (33.3%) compared with control plants. Our data indicate that excessive PCs and MTs without vacuolar sequestration enhance reactive oxygen species (ROS) generation. The findings of this investigation show that enhanced PCs and MTs owing to EDTA sequester excessive chromium in root vacuoles but, in the absence of sequestration due to a lack of chromium, enhance reactive oxygen species generation, resulting in toxicity. The outcomes of this investigation will open a new avenue of research in phytoextraction or phytoremediation activities.
format	article
author	A. K. M. Nazmul Huda Muktar Hossain Rumana Haque Mukta Mossammad Rima Khatun Md. Anwarul Haque
author_facet	A. K. M. Nazmul Huda Muktar Hossain Rumana Haque Mukta Mossammad Rima Khatun Md. Anwarul Haque
author_sort	A. K. M. Nazmul Huda
title	EDTA-enhanced Cr detoxification and its potential toxicity in rice (Oryza sativa L.)
title_short	EDTA-enhanced Cr detoxification and its potential toxicity in rice (Oryza sativa L.)
title_full	EDTA-enhanced Cr detoxification and its potential toxicity in rice (Oryza sativa L.)
title_fullStr	EDTA-enhanced Cr detoxification and its potential toxicity in rice (Oryza sativa L.)
title_full_unstemmed	EDTA-enhanced Cr detoxification and its potential toxicity in rice (Oryza sativa L.)
title_sort	edta-enhanced cr detoxification and its potential toxicity in rice (oryza sativa l.)
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0c7013adfd5145cbb9432fae8c6ca9b9
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EDTA-enhanced Cr detoxification and its potential toxicity in rice (Oryza sativa L.)

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