The Drosophila Translational Control Element (TCE) is required for high-level transcription of many genes that are specifically expressed in testes.

To investigate the importance of core promoter elements for tissue-specific transcription of RNA polymerase II genes, we examined testis-specific transcription in Drosophila melanogaster. Bioinformatic analyses of core promoter sequences from 190 genes that are specifically expressed in testes ident...

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Autores principales:	Rebeccah J Katzenberger, Elizabeth A Rach, Ashley K Anderson, Uwe Ohler, David A Wassarman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c70bc9bd72940e186f061d673742ed8
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spelling	oai:doaj.org-article:0c70bc9bd72940e186f061d673742ed82021-11-18T07:05:59ZThe Drosophila Translational Control Element (TCE) is required for high-level transcription of many genes that are specifically expressed in testes.1932-620310.1371/journal.pone.0045009https://doaj.org/article/0c70bc9bd72940e186f061d673742ed82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22984601/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203To investigate the importance of core promoter elements for tissue-specific transcription of RNA polymerase II genes, we examined testis-specific transcription in Drosophila melanogaster. Bioinformatic analyses of core promoter sequences from 190 genes that are specifically expressed in testes identified a 10 bp A/T-rich motif that is identical to the translational control element (TCE). The TCE functions in the 5' untranslated region of Mst(3)CGP mRNAs to repress translation, and it also functions in a heterologous gene to regulate transcription. We found that among genes with focused initiation patterns, the TCE is significantly enriched in core promoters of genes that are specifically expressed in testes but not in core promoters of genes that are specifically expressed in other tissues. The TCE is variably located in core promoters and is conserved in melanogaster subgroup species, but conservation dramatically drops in more distant species. In transgenic flies, short (300-400 bp) genomic regions containing a TCE directed testis-specific transcription of a reporter gene. Mutation of the TCE significantly reduced but did not abolish reporter gene transcription indicating that the TCE is important but not essential for transcription activation. Finally, mutation of testis-specific TFIID (tTFIID) subunits significantly reduced the transcription of a subset of endogenous TCE-containing but not TCE-lacking genes, suggesting that tTFIID activity is limited to TCE-containing genes but that tTFIID is not an obligatory regulator of TCE-containing genes. Thus, the TCE is a core promoter element in a subset of genes that are specifically expressed in testes. Furthermore, the TCE regulates transcription in the context of short genomic regions, from variable locations in the core promoter, and both dependently and independently of tTFIID. These findings set the stage for determining the mechanism by which the TCE regulates testis-specific transcription and understanding the dual role of the TCE in translational and transcriptional regulation.Rebeccah J KatzenbergerElizabeth A RachAshley K AndersonUwe OhlerDavid A WassarmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45009 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rebeccah J Katzenberger Elizabeth A Rach Ashley K Anderson Uwe Ohler David A Wassarman The Drosophila Translational Control Element (TCE) is required for high-level transcription of many genes that are specifically expressed in testes.
description	To investigate the importance of core promoter elements for tissue-specific transcription of RNA polymerase II genes, we examined testis-specific transcription in Drosophila melanogaster. Bioinformatic analyses of core promoter sequences from 190 genes that are specifically expressed in testes identified a 10 bp A/T-rich motif that is identical to the translational control element (TCE). The TCE functions in the 5' untranslated region of Mst(3)CGP mRNAs to repress translation, and it also functions in a heterologous gene to regulate transcription. We found that among genes with focused initiation patterns, the TCE is significantly enriched in core promoters of genes that are specifically expressed in testes but not in core promoters of genes that are specifically expressed in other tissues. The TCE is variably located in core promoters and is conserved in melanogaster subgroup species, but conservation dramatically drops in more distant species. In transgenic flies, short (300-400 bp) genomic regions containing a TCE directed testis-specific transcription of a reporter gene. Mutation of the TCE significantly reduced but did not abolish reporter gene transcription indicating that the TCE is important but not essential for transcription activation. Finally, mutation of testis-specific TFIID (tTFIID) subunits significantly reduced the transcription of a subset of endogenous TCE-containing but not TCE-lacking genes, suggesting that tTFIID activity is limited to TCE-containing genes but that tTFIID is not an obligatory regulator of TCE-containing genes. Thus, the TCE is a core promoter element in a subset of genes that are specifically expressed in testes. Furthermore, the TCE regulates transcription in the context of short genomic regions, from variable locations in the core promoter, and both dependently and independently of tTFIID. These findings set the stage for determining the mechanism by which the TCE regulates testis-specific transcription and understanding the dual role of the TCE in translational and transcriptional regulation.
format	article
author	Rebeccah J Katzenberger Elizabeth A Rach Ashley K Anderson Uwe Ohler David A Wassarman
author_facet	Rebeccah J Katzenberger Elizabeth A Rach Ashley K Anderson Uwe Ohler David A Wassarman
author_sort	Rebeccah J Katzenberger
title	The Drosophila Translational Control Element (TCE) is required for high-level transcription of many genes that are specifically expressed in testes.
title_short	The Drosophila Translational Control Element (TCE) is required for high-level transcription of many genes that are specifically expressed in testes.
title_full	The Drosophila Translational Control Element (TCE) is required for high-level transcription of many genes that are specifically expressed in testes.
title_fullStr	The Drosophila Translational Control Element (TCE) is required for high-level transcription of many genes that are specifically expressed in testes.
title_full_unstemmed	The Drosophila Translational Control Element (TCE) is required for high-level transcription of many genes that are specifically expressed in testes.
title_sort	drosophila translational control element (tce) is required for high-level transcription of many genes that are specifically expressed in testes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0c70bc9bd72940e186f061d673742ed8
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The Drosophila Translational Control Element (TCE) is required for high-level transcription of many genes that are specifically expressed in testes.

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