Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum.
Quorum sensing (QS) in vitro controls production of plant cell wall degrading enzymes (PCWDEs) and other virulence factors in the soft rotting enterobacterial plant pathogen Pectobacterium atrosepticum (Pba). Here, we demonstrate the genome-wide regulatory role of QS in vivo during the Pba-potato in...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2008
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/0c71679f557542aa88c15f674a35a9be |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:0c71679f557542aa88c15f674a35a9be |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:0c71679f557542aa88c15f674a35a9be2021-11-25T05:46:32ZQuorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum.1553-73661553-737410.1371/journal.ppat.1000093https://doaj.org/article/0c71679f557542aa88c15f674a35a9be2008-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18566662/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Quorum sensing (QS) in vitro controls production of plant cell wall degrading enzymes (PCWDEs) and other virulence factors in the soft rotting enterobacterial plant pathogen Pectobacterium atrosepticum (Pba). Here, we demonstrate the genome-wide regulatory role of QS in vivo during the Pba-potato interaction, using a Pba-specific microarray. We show that 26% of the Pba genome exhibited differential transcription in a QS (expI-) mutant, compared to the wild-type, suggesting that QS may make a greater contribution to pathogenesis than previously thought. We identify novel components of the QS regulon, including the Type I and II secretion systems, which are involved in the secretion of PCWDEs; a novel Type VI secretion system (T6SS) and its predicted substrates Hcp and VgrG; more than 70 known or putative regulators, some of which have been demonstrated to control pathogenesis and, remarkably, the Type III secretion system and associated effector proteins, and coronafacoyl-amide conjugates, both of which play roles in the manipulation of plant defences. We show that the T6SS and a novel potential regulator, VirS, are required for full virulence in Pba, and propose a model placing QS at the apex of a regulatory hierarchy controlling the later stages of disease progression in Pba. Our findings indicate that QS is a master regulator of phytopathogenesis, controlling multiple other regulators that, in turn, co-ordinately regulate genes associated with manipulation of host defences in concert with the destructive arsenal of PCWDEs that manifest the soft rot disease phenotype.Hui LiuSarah J CoulthurstLeighton PritchardPeter E HedleyMichael RavensdaleSonia HumphrisTom BurrGunnhild TakleMay-Bente BrurbergPaul R J BirchGeorge P C SalmondIan K TothPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 4, Iss 6, p e1000093 (2008) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Hui Liu Sarah J Coulthurst Leighton Pritchard Peter E Hedley Michael Ravensdale Sonia Humphris Tom Burr Gunnhild Takle May-Bente Brurberg Paul R J Birch George P C Salmond Ian K Toth Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. |
| description |
Quorum sensing (QS) in vitro controls production of plant cell wall degrading enzymes (PCWDEs) and other virulence factors in the soft rotting enterobacterial plant pathogen Pectobacterium atrosepticum (Pba). Here, we demonstrate the genome-wide regulatory role of QS in vivo during the Pba-potato interaction, using a Pba-specific microarray. We show that 26% of the Pba genome exhibited differential transcription in a QS (expI-) mutant, compared to the wild-type, suggesting that QS may make a greater contribution to pathogenesis than previously thought. We identify novel components of the QS regulon, including the Type I and II secretion systems, which are involved in the secretion of PCWDEs; a novel Type VI secretion system (T6SS) and its predicted substrates Hcp and VgrG; more than 70 known or putative regulators, some of which have been demonstrated to control pathogenesis and, remarkably, the Type III secretion system and associated effector proteins, and coronafacoyl-amide conjugates, both of which play roles in the manipulation of plant defences. We show that the T6SS and a novel potential regulator, VirS, are required for full virulence in Pba, and propose a model placing QS at the apex of a regulatory hierarchy controlling the later stages of disease progression in Pba. Our findings indicate that QS is a master regulator of phytopathogenesis, controlling multiple other regulators that, in turn, co-ordinately regulate genes associated with manipulation of host defences in concert with the destructive arsenal of PCWDEs that manifest the soft rot disease phenotype. |
| format |
article |
| author |
Hui Liu Sarah J Coulthurst Leighton Pritchard Peter E Hedley Michael Ravensdale Sonia Humphris Tom Burr Gunnhild Takle May-Bente Brurberg Paul R J Birch George P C Salmond Ian K Toth |
| author_facet |
Hui Liu Sarah J Coulthurst Leighton Pritchard Peter E Hedley Michael Ravensdale Sonia Humphris Tom Burr Gunnhild Takle May-Bente Brurberg Paul R J Birch George P C Salmond Ian K Toth |
| author_sort |
Hui Liu |
| title |
Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. |
| title_short |
Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. |
| title_full |
Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. |
| title_fullStr |
Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. |
| title_full_unstemmed |
Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. |
| title_sort |
quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen pectobacterium atrosepticum. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2008 |
| url |
https://doaj.org/article/0c71679f557542aa88c15f674a35a9be |
| work_keys_str_mv |
AT huiliu quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT sarahjcoulthurst quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT leightonpritchard quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT peterehedley quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT michaelravensdale quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT soniahumphris quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT tomburr quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT gunnhildtakle quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT maybentebrurberg quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT paulrjbirch quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT georgepcsalmond quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum AT ianktoth quorumsensingcoordinatesbruteforceandstealthmodesofinfectionintheplantpathogenpectobacteriumatrosepticum |
| _version_ |
1718414443051745280 |