ACUTE ENTERIC INFECTIONS POLYMERASE CHAIN REACTION ASSAY IN PEDIATRIC PRACTICE: OPPORTUNITIES AND CHALLENGES

The aim of the study is estimate the opportunities of local multi-prime PCR reagents kits in children enteric infections etiological diagnostics amongst the patients with diarrhoea vs traditional bacteriological methods. We used 4 kits of reagents that provide multiple pathogens simultaneous indicat...

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Autores principales:	E. D. Sokolova, A. M. Galtaeva, O. U. Zamurei, O. V. Didichenko, U. V. Sokolova, V. A. Muratova, O. U. Ligorova, I. N. Zhuravleva, M. A. Makarova, L. A. Kaftyreva
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2016
Materias:	enteric infections pcr diagnostics enteropatogens rotavirus norovirus salmonella spp. e. coli campylobacter spp. yersinia Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/0c77eef5d7ac47548b185c766f5cd7d5
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spelling	oai:doaj.org-article:0c77eef5d7ac47548b185c766f5cd7d52021-11-22T07:09:48ZACUTE ENTERIC INFECTIONS POLYMERASE CHAIN REACTION ASSAY IN PEDIATRIC PRACTICE: OPPORTUNITIES AND CHALLENGES2220-76192313-739810.15789/2220-7619-2016-3-225-231https://doaj.org/article/0c77eef5d7ac47548b185c766f5cd7d52016-09-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/422https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The aim of the study is estimate the opportunities of local multi-prime PCR reagents kits in children enteric infections etiological diagnostics amongst the patients with diarrhoea vs traditional bacteriological methods. We used 4 kits of reagents that provide multiple pathogens simultaneous indication in one sample: 1) Rotavirus, Norovirus, Astrovirus; 2) Shigella spp./EIEC, Salmonella spp., Campylobacter spp.; 3) Yersinia enterocolitica and Yersinia pseudotuberculosis; 4) E. coli: EIEC (enteroinvasive), EPEC (enteropathogenic), ETEC (enterotoxigenic), EHEC (enterohaemorrhagic), EAgEC (enteroaggregative). It has been shown that the viral intestinal infections is increased by 14%, bacterial — in 2,5 times. PCR diagnostics identified in 62% of patients the viral gastroenteritis: Rotavirus (52%), Norovirus (9%), Astrovirus (1%). Detected bacterial pathogens PCR markers number proved up to 2.5 times high than according to bacteriological examination. The spectrum of bacterial agents increased due to E. coli and Y. enterocolitica. PCR diagnostics increased detection of Campylobacter up to 2 times. Detected E. coli DNA prevalence: EPEC — 66%, EAgEC, ETEC and EHEC were 31%, 9% and 4%, respectively. DNA Campylobacter spp. and E. coli constituted 2/3 of all findings: Campylobacter spp. (41%), E. coli (24%), Salmonella spp. (19%), Yersinia spp. (11%), Shigella spp./EIEC (5%). The positive results of bacteriological and serological methods duplicate the positive results of PCR diagnostics. In general, the positive results of PCR diagnosis of bacterial pathogens were detected in 46.35% of the examined patients. In 48.4% of patients identified PCR markers viral — bacterial infection, in 5.25% — of bacterial associations, in 11% of them were found the DNA 2–3 bacterial pathogens. The study was shown in children in St. Petersburg in 2012–2014 dominated rotavirus infection, campylobacteriosis and escherichiosis. The prevalence of viral-bacterial confections is more than 50% of all diagnosed cases.E. D. SokolovaA. M. GaltaevaO. U. ZamureiO. V. DidichenkoU. V. SokolovaV. A. MuratovaO. U. LigorovaI. N. ZhuravlevaM. A. MakarovaL. A. KaftyrevaSankt-Peterburg : NIIÈM imeni Pasteraarticleenteric infectionspcr diagnosticsenteropatogensrotavirusnorovirussalmonella spp.e. colicampylobacter spp.yersiniaInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 6, Iss 3, Pp 225-231 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	enteric infections pcr diagnostics enteropatogens rotavirus norovirus salmonella spp. e. coli campylobacter spp. yersinia Infectious and parasitic diseases RC109-216
spellingShingle	enteric infections pcr diagnostics enteropatogens rotavirus norovirus salmonella spp. e. coli campylobacter spp. yersinia Infectious and parasitic diseases RC109-216 E. D. Sokolova A. M. Galtaeva O. U. Zamurei O. V. Didichenko U. V. Sokolova V. A. Muratova O. U. Ligorova I. N. Zhuravleva M. A. Makarova L. A. Kaftyreva ACUTE ENTERIC INFECTIONS POLYMERASE CHAIN REACTION ASSAY IN PEDIATRIC PRACTICE: OPPORTUNITIES AND CHALLENGES
description	The aim of the study is estimate the opportunities of local multi-prime PCR reagents kits in children enteric infections etiological diagnostics amongst the patients with diarrhoea vs traditional bacteriological methods. We used 4 kits of reagents that provide multiple pathogens simultaneous indication in one sample: 1) Rotavirus, Norovirus, Astrovirus; 2) Shigella spp./EIEC, Salmonella spp., Campylobacter spp.; 3) Yersinia enterocolitica and Yersinia pseudotuberculosis; 4) E. coli: EIEC (enteroinvasive), EPEC (enteropathogenic), ETEC (enterotoxigenic), EHEC (enterohaemorrhagic), EAgEC (enteroaggregative). It has been shown that the viral intestinal infections is increased by 14%, bacterial — in 2,5 times. PCR diagnostics identified in 62% of patients the viral gastroenteritis: Rotavirus (52%), Norovirus (9%), Astrovirus (1%). Detected bacterial pathogens PCR markers number proved up to 2.5 times high than according to bacteriological examination. The spectrum of bacterial agents increased due to E. coli and Y. enterocolitica. PCR diagnostics increased detection of Campylobacter up to 2 times. Detected E. coli DNA prevalence: EPEC — 66%, EAgEC, ETEC and EHEC were 31%, 9% and 4%, respectively. DNA Campylobacter spp. and E. coli constituted 2/3 of all findings: Campylobacter spp. (41%), E. coli (24%), Salmonella spp. (19%), Yersinia spp. (11%), Shigella spp./EIEC (5%). The positive results of bacteriological and serological methods duplicate the positive results of PCR diagnostics. In general, the positive results of PCR diagnosis of bacterial pathogens were detected in 46.35% of the examined patients. In 48.4% of patients identified PCR markers viral — bacterial infection, in 5.25% — of bacterial associations, in 11% of them were found the DNA 2–3 bacterial pathogens. The study was shown in children in St. Petersburg in 2012–2014 dominated rotavirus infection, campylobacteriosis and escherichiosis. The prevalence of viral-bacterial confections is more than 50% of all diagnosed cases.
format	article
author	E. D. Sokolova A. M. Galtaeva O. U. Zamurei O. V. Didichenko U. V. Sokolova V. A. Muratova O. U. Ligorova I. N. Zhuravleva M. A. Makarova L. A. Kaftyreva
author_facet	E. D. Sokolova A. M. Galtaeva O. U. Zamurei O. V. Didichenko U. V. Sokolova V. A. Muratova O. U. Ligorova I. N. Zhuravleva M. A. Makarova L. A. Kaftyreva
author_sort	E. D. Sokolova
title	ACUTE ENTERIC INFECTIONS POLYMERASE CHAIN REACTION ASSAY IN PEDIATRIC PRACTICE: OPPORTUNITIES AND CHALLENGES
title_short	ACUTE ENTERIC INFECTIONS POLYMERASE CHAIN REACTION ASSAY IN PEDIATRIC PRACTICE: OPPORTUNITIES AND CHALLENGES
title_full	ACUTE ENTERIC INFECTIONS POLYMERASE CHAIN REACTION ASSAY IN PEDIATRIC PRACTICE: OPPORTUNITIES AND CHALLENGES
title_fullStr	ACUTE ENTERIC INFECTIONS POLYMERASE CHAIN REACTION ASSAY IN PEDIATRIC PRACTICE: OPPORTUNITIES AND CHALLENGES
title_full_unstemmed	ACUTE ENTERIC INFECTIONS POLYMERASE CHAIN REACTION ASSAY IN PEDIATRIC PRACTICE: OPPORTUNITIES AND CHALLENGES
title_sort	acute enteric infections polymerase chain reaction assay in pediatric practice: opportunities and challenges
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2016
url	https://doaj.org/article/0c77eef5d7ac47548b185c766f5cd7d5
work_keys_str_mv	AT edsokolova acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges AT amgaltaeva acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges AT ouzamurei acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges AT ovdidichenko acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges AT uvsokolova acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges AT vamuratova acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges AT ouligorova acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges AT inzhuravleva acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges AT mamakarova acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges AT lakaftyreva acuteentericinfectionspolymerasechainreactionassayinpediatricpracticeopportunitiesandchallenges
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ACUTE ENTERIC INFECTIONS POLYMERASE CHAIN REACTION ASSAY IN PEDIATRIC PRACTICE: OPPORTUNITIES AND CHALLENGES

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