Spatiotemporal variation of the indoor mycobiome in daycare centers

Abstract Background Children spend considerable time in daycare centers in parts of the world and are exposed to the indoor micro- and mycobiomes of these facilities. The level of exposure to microorganisms varies within and between buildings, depending on occupancy, climate, and season. In order to...

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Autores principales:	Eva Lena F. Estensmo, Luis Morgado, Sundy Maurice, Pedro M. Martin-Sanchez, Ingeborg B. Engh, Johan Mattsson, Håvard Kauserud, Inger Skrede
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Fungi Seasonal variation DNA metabarcoding Dust mycobiome Built environment Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/0c79e26647324dbd88799cbce13dce1d
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spelling	oai:doaj.org-article:0c79e26647324dbd88799cbce13dce1d2021-11-14T12:10:14ZSpatiotemporal variation of the indoor mycobiome in daycare centers10.1186/s40168-021-01167-x2049-2618https://doaj.org/article/0c79e26647324dbd88799cbce13dce1d2021-11-01T00:00:00Zhttps://doi.org/10.1186/s40168-021-01167-xhttps://doaj.org/toc/2049-2618Abstract Background Children spend considerable time in daycare centers in parts of the world and are exposed to the indoor micro- and mycobiomes of these facilities. The level of exposure to microorganisms varies within and between buildings, depending on occupancy, climate, and season. In order to evaluate indoor air quality, and the effect of usage and seasonality, we investigated the spatiotemporal variation in the indoor mycobiomes of two daycare centers. We collected dust samples from different rooms throughout a year and analyzed their mycobiomes using DNA metabarcoding. Results The fungal community composition in rooms with limited occupancy (auxiliary rooms) was similar to the outdoor samples, and clearly different from the rooms with higher occupancy (main rooms). The main rooms had higher abundance of Ascomycota, while the auxiliary rooms contained comparably more Basidiomycota. We observed a strong seasonal pattern in the mycobiome composition, mainly structured by the outdoor climate. Most markedly, basidiomycetes of the orders Agaricales and Polyporales, mainly reflecting typical outdoor fungi, were more abundant during summer and fall. In contrast, ascomycetes of the orders Saccharomycetales and Capnodiales were dominant during winter and spring. Conclusions Our findings provide clear evidences that the indoor mycobiomes in daycare centers are structured by occupancy as well as outdoor seasonality. We conclude that the temporal variability should be accounted for in indoor mycobiome studies and in the evaluation of indoor air quality of buildings. Video abstractEva Lena F. EstensmoLuis MorgadoSundy MauricePedro M. Martin-SanchezIngeborg B. EnghJohan MattssonHåvard KauserudInger SkredeBMCarticleFungiSeasonal variationDNA metabarcodingDust mycobiomeBuilt environmentMicrobial ecologyMicrobial ecologyQR100-130ENMicrobiome, Vol 9, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fungi Seasonal variation DNA metabarcoding Dust mycobiome Built environment Microbial ecology Microbial ecology QR100-130
spellingShingle	Fungi Seasonal variation DNA metabarcoding Dust mycobiome Built environment Microbial ecology Microbial ecology QR100-130 Eva Lena F. Estensmo Luis Morgado Sundy Maurice Pedro M. Martin-Sanchez Ingeborg B. Engh Johan Mattsson Håvard Kauserud Inger Skrede Spatiotemporal variation of the indoor mycobiome in daycare centers
description	Abstract Background Children spend considerable time in daycare centers in parts of the world and are exposed to the indoor micro- and mycobiomes of these facilities. The level of exposure to microorganisms varies within and between buildings, depending on occupancy, climate, and season. In order to evaluate indoor air quality, and the effect of usage and seasonality, we investigated the spatiotemporal variation in the indoor mycobiomes of two daycare centers. We collected dust samples from different rooms throughout a year and analyzed their mycobiomes using DNA metabarcoding. Results The fungal community composition in rooms with limited occupancy (auxiliary rooms) was similar to the outdoor samples, and clearly different from the rooms with higher occupancy (main rooms). The main rooms had higher abundance of Ascomycota, while the auxiliary rooms contained comparably more Basidiomycota. We observed a strong seasonal pattern in the mycobiome composition, mainly structured by the outdoor climate. Most markedly, basidiomycetes of the orders Agaricales and Polyporales, mainly reflecting typical outdoor fungi, were more abundant during summer and fall. In contrast, ascomycetes of the orders Saccharomycetales and Capnodiales were dominant during winter and spring. Conclusions Our findings provide clear evidences that the indoor mycobiomes in daycare centers are structured by occupancy as well as outdoor seasonality. We conclude that the temporal variability should be accounted for in indoor mycobiome studies and in the evaluation of indoor air quality of buildings. Video abstract
format	article
author	Eva Lena F. Estensmo Luis Morgado Sundy Maurice Pedro M. Martin-Sanchez Ingeborg B. Engh Johan Mattsson Håvard Kauserud Inger Skrede
author_facet	Eva Lena F. Estensmo Luis Morgado Sundy Maurice Pedro M. Martin-Sanchez Ingeborg B. Engh Johan Mattsson Håvard Kauserud Inger Skrede
author_sort	Eva Lena F. Estensmo
title	Spatiotemporal variation of the indoor mycobiome in daycare centers
title_short	Spatiotemporal variation of the indoor mycobiome in daycare centers
title_full	Spatiotemporal variation of the indoor mycobiome in daycare centers
title_fullStr	Spatiotemporal variation of the indoor mycobiome in daycare centers
title_full_unstemmed	Spatiotemporal variation of the indoor mycobiome in daycare centers
title_sort	spatiotemporal variation of the indoor mycobiome in daycare centers
publisher	BMC
publishDate	2021
url	https://doaj.org/article/0c79e26647324dbd88799cbce13dce1d
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Spatiotemporal variation of the indoor mycobiome in daycare centers

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