Polarized Distribution of Active Myosin II Regulates Directional Migration of Cultured Olfactory Ensheathing Cells

Abstract Migration of olfactory ensheathing cells (OECs) is critical for development of olfactory system and essential for neural regeneration after OEC transplantation into nerve injury site. However, the molecular mechanisms underlying the regulation of directional migration of OECs remain unclear...

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Autores principales:	Cheng-gen Zheng, Fan Zhang, Xiao-mei Bao, Shi-yang Wu, Peng Wang, Jia-nan Zhou, Yuan Gao, Hong-lin Teng, Ying Wang, Zhi-hui Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c8016c0835b43ea929d4674e90ad797
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spelling	oai:doaj.org-article:0c8016c0835b43ea929d4674e90ad7972021-12-02T15:05:59ZPolarized Distribution of Active Myosin II Regulates Directional Migration of Cultured Olfactory Ensheathing Cells10.1038/s41598-017-04914-z2045-2322https://doaj.org/article/0c8016c0835b43ea929d4674e90ad7972017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04914-zhttps://doaj.org/toc/2045-2322Abstract Migration of olfactory ensheathing cells (OECs) is critical for development of olfactory system and essential for neural regeneration after OEC transplantation into nerve injury site. However, the molecular mechanisms underlying the regulation of directional migration of OECs remain unclear. In this study, we found that in migrating OECs, phosphorylated myosin light chain (p-MLC, active myosin II) displayed a polarized distribution, with the leading front exhibiting higher than soma and trailing process. Over-expression of GFP-MLC significantly reduced OEC migration. Moreover, decreasing this front-to-rear difference of myosin II activity by the frontal application of a ML-7 (myosin II inhibitors) gradient induced the collapse of leading front and reversed soma translocation of OECs, whereas, increasing this front-to-rear difference of myosin II activity by the rear application of a ML-7 or BDM gradient or the frontal application of a Caly (myosin II activator) gradient accelerated the soma translocation of OECs. Finally, myosin II as a downstream signaling of repulsive factor Slit-2 mediated the reversal of soma translocation induced by Slit-2. Taken together, these results suggest that the polarized distribution of active myosin II regulates the directional migration of OECs during spontaneous migration or upon to extracellular stimulation such as Slit-2.Cheng-gen ZhengFan ZhangXiao-mei BaoShi-yang WuPeng WangJia-nan ZhouYuan GaoHong-lin TengYing WangZhi-hui HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cheng-gen Zheng Fan Zhang Xiao-mei Bao Shi-yang Wu Peng Wang Jia-nan Zhou Yuan Gao Hong-lin Teng Ying Wang Zhi-hui Huang Polarized Distribution of Active Myosin II Regulates Directional Migration of Cultured Olfactory Ensheathing Cells
description	Abstract Migration of olfactory ensheathing cells (OECs) is critical for development of olfactory system and essential for neural regeneration after OEC transplantation into nerve injury site. However, the molecular mechanisms underlying the regulation of directional migration of OECs remain unclear. In this study, we found that in migrating OECs, phosphorylated myosin light chain (p-MLC, active myosin II) displayed a polarized distribution, with the leading front exhibiting higher than soma and trailing process. Over-expression of GFP-MLC significantly reduced OEC migration. Moreover, decreasing this front-to-rear difference of myosin II activity by the frontal application of a ML-7 (myosin II inhibitors) gradient induced the collapse of leading front and reversed soma translocation of OECs, whereas, increasing this front-to-rear difference of myosin II activity by the rear application of a ML-7 or BDM gradient or the frontal application of a Caly (myosin II activator) gradient accelerated the soma translocation of OECs. Finally, myosin II as a downstream signaling of repulsive factor Slit-2 mediated the reversal of soma translocation induced by Slit-2. Taken together, these results suggest that the polarized distribution of active myosin II regulates the directional migration of OECs during spontaneous migration or upon to extracellular stimulation such as Slit-2.
format	article
author	Cheng-gen Zheng Fan Zhang Xiao-mei Bao Shi-yang Wu Peng Wang Jia-nan Zhou Yuan Gao Hong-lin Teng Ying Wang Zhi-hui Huang
author_facet	Cheng-gen Zheng Fan Zhang Xiao-mei Bao Shi-yang Wu Peng Wang Jia-nan Zhou Yuan Gao Hong-lin Teng Ying Wang Zhi-hui Huang
author_sort	Cheng-gen Zheng
title	Polarized Distribution of Active Myosin II Regulates Directional Migration of Cultured Olfactory Ensheathing Cells
title_short	Polarized Distribution of Active Myosin II Regulates Directional Migration of Cultured Olfactory Ensheathing Cells
title_full	Polarized Distribution of Active Myosin II Regulates Directional Migration of Cultured Olfactory Ensheathing Cells
title_fullStr	Polarized Distribution of Active Myosin II Regulates Directional Migration of Cultured Olfactory Ensheathing Cells
title_full_unstemmed	Polarized Distribution of Active Myosin II Regulates Directional Migration of Cultured Olfactory Ensheathing Cells
title_sort	polarized distribution of active myosin ii regulates directional migration of cultured olfactory ensheathing cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0c8016c0835b43ea929d4674e90ad797
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_version_	1718388624660103168

Polarized Distribution of Active Myosin II Regulates Directional Migration of Cultured Olfactory Ensheathing Cells

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