Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978

Acinetobacter baumannii is a nosocomial pathogen that causes multi-drug resistant infections mainly in immunocompromised patients. Although this gram-negative species lacks flagella, it is able to move over wet surfaces through a not well characterized type of movement known as surface-associated mo...

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Autores principales:	Jordi Corral, María Pérez-Varela, Jordi Barbé, Jesús Aranda
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	a. baumannii surface-associated motility chemotaxis virulence reca chew-like protein Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/0c89b51c9ae14c41bce9b7916e02d0ad
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spelling	oai:doaj.org-article:0c89b51c9ae14c41bce9b7916e02d0ad2021-11-17T14:21:58ZDirect interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 179782150-55942150-560810.1080/21505594.2020.1748923https://doaj.org/article/0c89b51c9ae14c41bce9b7916e02d0ad2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1748923https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Acinetobacter baumannii is a nosocomial pathogen that causes multi-drug resistant infections mainly in immunocompromised patients. Although this gram-negative species lacks flagella, it is able to move over wet surfaces through a not well characterized type of movement known as surface-associated motility. In this study we demonstrate through the inactivation of the A1S_2813 gene (coding a CheW-like protein) and recA (coding a DNA damage repair and recombination protein) that both genes are involved in the surface-associated motility and chemotaxis of A. baumannii ATCC 17978 strain. In addition, we also point out that the lack of either RecA or CheW-like proteins reduces its virulence in the Caenorhabditis elegans and the Galleria mellonella animal models. Furthermore, we show through co-immunoprecipitation assays that the CheW-like protein and RecA interact and that this interaction is abolished by the introduction of the mutation S97A in one of the domains of CheW-like protein that is structurally conserved in Salmonella enterica and necessary for the RecA-CheW interaction in this bacterial species. Finally, we show that the replacement of the wild-type CheW-like protein by that presenting the S97A mutation impairs surface-associated motility, chemotaxis and virulence of A. baumannii strain ATCC 17978.Jordi CorralMaría Pérez-VarelaJordi BarbéJesús ArandaTaylor & Francis Grouparticlea. baumanniisurface-associated motilitychemotaxisvirulencerecachew-like proteinInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 315-326 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	a. baumannii surface-associated motility chemotaxis virulence reca chew-like protein Infectious and parasitic diseases RC109-216
spellingShingle	a. baumannii surface-associated motility chemotaxis virulence reca chew-like protein Infectious and parasitic diseases RC109-216 Jordi Corral María Pérez-Varela Jordi Barbé Jesús Aranda Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978
description	Acinetobacter baumannii is a nosocomial pathogen that causes multi-drug resistant infections mainly in immunocompromised patients. Although this gram-negative species lacks flagella, it is able to move over wet surfaces through a not well characterized type of movement known as surface-associated motility. In this study we demonstrate through the inactivation of the A1S_2813 gene (coding a CheW-like protein) and recA (coding a DNA damage repair and recombination protein) that both genes are involved in the surface-associated motility and chemotaxis of A. baumannii ATCC 17978 strain. In addition, we also point out that the lack of either RecA or CheW-like proteins reduces its virulence in the Caenorhabditis elegans and the Galleria mellonella animal models. Furthermore, we show through co-immunoprecipitation assays that the CheW-like protein and RecA interact and that this interaction is abolished by the introduction of the mutation S97A in one of the domains of CheW-like protein that is structurally conserved in Salmonella enterica and necessary for the RecA-CheW interaction in this bacterial species. Finally, we show that the replacement of the wild-type CheW-like protein by that presenting the S97A mutation impairs surface-associated motility, chemotaxis and virulence of A. baumannii strain ATCC 17978.
format	article
author	Jordi Corral María Pérez-Varela Jordi Barbé Jesús Aranda
author_facet	Jordi Corral María Pérez-Varela Jordi Barbé Jesús Aranda
author_sort	Jordi Corral
title	Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978
title_short	Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978
title_full	Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978
title_fullStr	Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978
title_full_unstemmed	Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978
title_sort	direct interaction between reca and a chew-like protein is required for surface-associated motility, chemotaxis and the full virulence of acinetobacter baumannii strain atcc 17978
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/0c89b51c9ae14c41bce9b7916e02d0ad
work_keys_str_mv	AT jordicorral directinteractionbetweenrecaandachewlikeproteinisrequiredforsurfaceassociatedmotilitychemotaxisandthefullvirulenceofacinetobacterbaumanniistrainatcc17978 AT mariaperezvarela directinteractionbetweenrecaandachewlikeproteinisrequiredforsurfaceassociatedmotilitychemotaxisandthefullvirulenceofacinetobacterbaumanniistrainatcc17978 AT jordibarbe directinteractionbetweenrecaandachewlikeproteinisrequiredforsurfaceassociatedmotilitychemotaxisandthefullvirulenceofacinetobacterbaumanniistrainatcc17978 AT jesusaranda directinteractionbetweenrecaandachewlikeproteinisrequiredforsurfaceassociatedmotilitychemotaxisandthefullvirulenceofacinetobacterbaumanniistrainatcc17978
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Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978

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