Decreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients

Abstract Programmed death ligand 1 (PD-L1) overexpression has been associated with poor clinical outcomes in several human cancers whose increased malignant behaviour might be related to PD-L1 mediated systemic immunological tolerance. This study aims to verify if circulating cytokines may serve as...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zuzana Cierna, Bozena Smolkova, Dana Cholujova, Paulina Gronesova, Svetlana Miklikova, Marina Cihova, Jana Plava, Michal Mego
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c8a61ac91ed49e2ab85bcac9794a1e9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0c8a61ac91ed49e2ab85bcac9794a1e9
record_format	dspace
spelling	oai:doaj.org-article:0c8a61ac91ed49e2ab85bcac9794a1e92021-12-02T14:01:24ZDecreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients10.1038/s41598-020-80351-92045-2322https://doaj.org/article/0c8a61ac91ed49e2ab85bcac9794a1e92021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80351-9https://doaj.org/toc/2045-2322Abstract Programmed death ligand 1 (PD-L1) overexpression has been associated with poor clinical outcomes in several human cancers whose increased malignant behaviour might be related to PD-L1 mediated systemic immunological tolerance. This study aims to verify if circulating cytokines may serve as a proxy for non-invasive identification of sensitive prognostic biomarkers reflecting tumour and its microenvironment. Immunohistochemistry was used to measure PD-L1 expression in tumour tissue sections of 148 chemonaïve breast cancer (BC) patients. The panel of 51 cytokines was analysed using multiplex bead arrays. High PD-L1 expression in tumours was associated with shorter progression-free survival (HR 3.25; 95% CI 1.39–7.61; P = 0.006) and low circulating levels of three multifunctional molecules; VEGF, TNF-β and IL-15 (P = 0.001). In multivariate analysis, patients with low VEGF had 4.6-fold increased risk of PD-L1 overexpression (P = 0.008), present in 76.5% of patients with all these three cytokines below the median (vs. 35.6% among the others; P = 0.002). The area under the curve value of 0.722 (95% CI 0.59–0.85; P = 0.004) shows that this combination of cytokines has a moderate ability to discriminate between PD-L1 high vs. PD-L1 low patients. Plasma cytokines, therefore, could serve as potential non-invasive biomarkers for the identification of high-risk BC cases.Zuzana CiernaBozena SmolkovaDana CholujovaPaulina GronesovaSvetlana MiklikovaMarina CihovaJana PlavaMichal MegoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zuzana Cierna Bozena Smolkova Dana Cholujova Paulina Gronesova Svetlana Miklikova Marina Cihova Jana Plava Michal Mego Decreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients
description	Abstract Programmed death ligand 1 (PD-L1) overexpression has been associated with poor clinical outcomes in several human cancers whose increased malignant behaviour might be related to PD-L1 mediated systemic immunological tolerance. This study aims to verify if circulating cytokines may serve as a proxy for non-invasive identification of sensitive prognostic biomarkers reflecting tumour and its microenvironment. Immunohistochemistry was used to measure PD-L1 expression in tumour tissue sections of 148 chemonaïve breast cancer (BC) patients. The panel of 51 cytokines was analysed using multiplex bead arrays. High PD-L1 expression in tumours was associated with shorter progression-free survival (HR 3.25; 95% CI 1.39–7.61; P = 0.006) and low circulating levels of three multifunctional molecules; VEGF, TNF-β and IL-15 (P = 0.001). In multivariate analysis, patients with low VEGF had 4.6-fold increased risk of PD-L1 overexpression (P = 0.008), present in 76.5% of patients with all these three cytokines below the median (vs. 35.6% among the others; P = 0.002). The area under the curve value of 0.722 (95% CI 0.59–0.85; P = 0.004) shows that this combination of cytokines has a moderate ability to discriminate between PD-L1 high vs. PD-L1 low patients. Plasma cytokines, therefore, could serve as potential non-invasive biomarkers for the identification of high-risk BC cases.
format	article
author	Zuzana Cierna Bozena Smolkova Dana Cholujova Paulina Gronesova Svetlana Miklikova Marina Cihova Jana Plava Michal Mego
author_facet	Zuzana Cierna Bozena Smolkova Dana Cholujova Paulina Gronesova Svetlana Miklikova Marina Cihova Jana Plava Michal Mego
author_sort	Zuzana Cierna
title	Decreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients
title_short	Decreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients
title_full	Decreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients
title_fullStr	Decreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients
title_full_unstemmed	Decreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients
title_sort	decreased levels of circulating cytokines vegf, tnf-β and il-15 indicate pd-l1 overexpression in tumours of primary breast cancer patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0c8a61ac91ed49e2ab85bcac9794a1e9
work_keys_str_mv	AT zuzanacierna decreasedlevelsofcirculatingcytokinesvegftnfbandil15indicatepdl1overexpressionintumoursofprimarybreastcancerpatients AT bozenasmolkova decreasedlevelsofcirculatingcytokinesvegftnfbandil15indicatepdl1overexpressionintumoursofprimarybreastcancerpatients AT danacholujova decreasedlevelsofcirculatingcytokinesvegftnfbandil15indicatepdl1overexpressionintumoursofprimarybreastcancerpatients AT paulinagronesova decreasedlevelsofcirculatingcytokinesvegftnfbandil15indicatepdl1overexpressionintumoursofprimarybreastcancerpatients AT svetlanamiklikova decreasedlevelsofcirculatingcytokinesvegftnfbandil15indicatepdl1overexpressionintumoursofprimarybreastcancerpatients AT marinacihova decreasedlevelsofcirculatingcytokinesvegftnfbandil15indicatepdl1overexpressionintumoursofprimarybreastcancerpatients AT janaplava decreasedlevelsofcirculatingcytokinesvegftnfbandil15indicatepdl1overexpressionintumoursofprimarybreastcancerpatients AT michalmego decreasedlevelsofcirculatingcytokinesvegftnfbandil15indicatepdl1overexpressionintumoursofprimarybreastcancerpatients
_version_	1718392122076299264

Decreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients

Ejemplares similares