Clinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p

Hong Zhang,1 Lihong Li,2 Leijie Xu,1 Yanyan Zheng1 1Department of Neonatology, Weifang Maternal and Child Health Hospital, Weifang, Shandong, People’s Republic of China; 2Department of Nursing, Weifang Maternal and Child Health Hospital, Weifang, Shandong, People’s Republic of ChinaCorrespondence: L...

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Autores principales:	Zhang H, Li L, Xu L, Zheng Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	norad mir-410-3p neonatal sepsis diagnosis inflammation Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0c9666f3abd546a69a28b2c9ed9188b4
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spelling	oai:doaj.org-article:0c9666f3abd546a69a28b2c9ed9188b42021-12-02T16:33:33ZClinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p1178-7031https://doaj.org/article/0c9666f3abd546a69a28b2c9ed9188b42021-08-01T00:00:00Zhttps://www.dovepress.com/clinical-significance-of-the-serum-lncrna-norad-expression-in-patients-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Hong Zhang,1 Lihong Li,2 Leijie Xu,1 Yanyan Zheng1 1Department of Neonatology, Weifang Maternal and Child Health Hospital, Weifang, Shandong, People’s Republic of China; 2Department of Nursing, Weifang Maternal and Child Health Hospital, Weifang, Shandong, People’s Republic of ChinaCorrespondence: Lihong LiDepartment of Nursing, Weifang Maternal and Child Health Hospital, Weifang, Shandong, People’s Republic of ChinaTel +86-536-8089019Fax +86-536-8089019Email liao69873@163.comPurpose: Neonatal sepsis (NS) is one of the most crucial causes of death in newborns. This investigation aimed to validate the expression level of NORAD and the probable mechanism underlying the function of NORAD in NS.Patients and Methods: The expression of NORAD and miR-410-3p was identified by qRT-PCR. The diagnostic sensitivity and specificity of NORAD were examined by the ROC curve. The NS cell models were established by the treatment of lipopolysaccharide (LPS) in the macrophage RAW264.7 cells. The luciferase report assay was performed to detect the target relationship between NORAD and miR-410-3p and the association between them was revealed by Pearson correlation.Results: The expression of NORAD was at a higher level in the NS group than in the pneumonia controls. The levels of NORAD could sever as a diagnostic marker on discriminating NS patients from pneumonia neonates. The expression of IL-6, IL-8, and TNF-α was enhanced in the macrophage cells under LPS circumstances, while NORAD knockdown reversed the overexpression of these pro-inflammatory cytokines. Besides, miR-410-3p was a possible ceRNA of NORAD by the finding that the luciferase activity fell in the co-transfection of miR-410-3p mimics and WT-NORAD group. In vitro, LPS management could inhibit the expression of miR-410-3p, while silenced NORAD ameliorated the suppressed miR-410-3p levels. Decreased expression of miR-410-3p was discovered in NS patients and the changes of miR-410-3p expression were correlated with the levels of NORAD in the NS patients.Conclusion: We found a raised level of NORAD in the NS patients and it might be a diagnostic indicator for NS patients. NORAD elimination meliorated the inflammation actions steered by LPS. MiR-410-3p was a target of NORAD and lowly expressed in the NS patients.Keywords: NORAD, miR-410-3p, neonatal sepsis, diagnosis, inflammationZhang HLi LXu LZheng YDove Medical Pressarticlenoradmir-410-3pneonatal sepsisdiagnosisinflammationPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 4181-4188 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	norad mir-410-3p neonatal sepsis diagnosis inflammation Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	norad mir-410-3p neonatal sepsis diagnosis inflammation Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Zhang H Li L Xu L Zheng Y Clinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p
description	Hong Zhang,1 Lihong Li,2 Leijie Xu,1 Yanyan Zheng1 1Department of Neonatology, Weifang Maternal and Child Health Hospital, Weifang, Shandong, People’s Republic of China; 2Department of Nursing, Weifang Maternal and Child Health Hospital, Weifang, Shandong, People’s Republic of ChinaCorrespondence: Lihong LiDepartment of Nursing, Weifang Maternal and Child Health Hospital, Weifang, Shandong, People’s Republic of ChinaTel +86-536-8089019Fax +86-536-8089019Email liao69873@163.comPurpose: Neonatal sepsis (NS) is one of the most crucial causes of death in newborns. This investigation aimed to validate the expression level of NORAD and the probable mechanism underlying the function of NORAD in NS.Patients and Methods: The expression of NORAD and miR-410-3p was identified by qRT-PCR. The diagnostic sensitivity and specificity of NORAD were examined by the ROC curve. The NS cell models were established by the treatment of lipopolysaccharide (LPS) in the macrophage RAW264.7 cells. The luciferase report assay was performed to detect the target relationship between NORAD and miR-410-3p and the association between them was revealed by Pearson correlation.Results: The expression of NORAD was at a higher level in the NS group than in the pneumonia controls. The levels of NORAD could sever as a diagnostic marker on discriminating NS patients from pneumonia neonates. The expression of IL-6, IL-8, and TNF-α was enhanced in the macrophage cells under LPS circumstances, while NORAD knockdown reversed the overexpression of these pro-inflammatory cytokines. Besides, miR-410-3p was a possible ceRNA of NORAD by the finding that the luciferase activity fell in the co-transfection of miR-410-3p mimics and WT-NORAD group. In vitro, LPS management could inhibit the expression of miR-410-3p, while silenced NORAD ameliorated the suppressed miR-410-3p levels. Decreased expression of miR-410-3p was discovered in NS patients and the changes of miR-410-3p expression were correlated with the levels of NORAD in the NS patients.Conclusion: We found a raised level of NORAD in the NS patients and it might be a diagnostic indicator for NS patients. NORAD elimination meliorated the inflammation actions steered by LPS. MiR-410-3p was a target of NORAD and lowly expressed in the NS patients.Keywords: NORAD, miR-410-3p, neonatal sepsis, diagnosis, inflammation
format	article
author	Zhang H Li L Xu L Zheng Y
author_facet	Zhang H Li L Xu L Zheng Y
author_sort	Zhang H
title	Clinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p
title_short	Clinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p
title_full	Clinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p
title_fullStr	Clinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p
title_full_unstemmed	Clinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p
title_sort	clinical significance of the serum lncrna norad expression in patients with neonatal sepsis and its association with mir-410-3p
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/0c9666f3abd546a69a28b2c9ed9188b4
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Clinical Significance of the Serum lncRNA NORAD Expression in Patients with Neonatal Sepsis and Its Association with miR-410-3p

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