Role of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells

Mohankandhasamy Ramasamy,1 Minakshi Das,1 Seong Soo A An,1 Dong Kee Yi2 1Division of Bionanotechnology, Gachon University, Seongnam, 2Department of Chemistry, Myongji University, Yongin, South Korea Abstract: The wide-scale applications of zinc oxide (ZnO) nanoparticles (NPs) in photocat...

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Autores principales:	Ramasamy M, Das M, An SSA, Yi DK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0c9a830d96154617958f13342b0f9db0
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spelling	oai:doaj.org-article:0c9a830d96154617958f13342b0f9db02021-12-02T00:12:17ZRole of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells1178-2013https://doaj.org/article/0c9a830d96154617958f13342b0f9db02014-08-01T00:00:00Zhttp://www.dovepress.com/role-of-surface-modification-in-zinc-oxide-nanoparticles-and-its-toxic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Mohankandhasamy Ramasamy,1 Minakshi Das,1 Seong Soo A An,1 Dong Kee Yi2 1Division of Bionanotechnology, Gachon University, Seongnam, 2Department of Chemistry, Myongji University, Yongin, South Korea Abstract: The wide-scale applications of zinc oxide (ZnO) nanoparticles (NPs) in photocatalysts, gas sensors, and cosmetics may cause toxicity to humans and environments. Therefore, the aim of the present study was to reduce the toxicity of ZnO NPs by coating them with a silica (SiO2) layer, which could be used in human applications, such as cosmetic preparations. The sol–gel method was used to synthesize core ZnO with SiO2-shelled NPs (SiO2/ZnO NPs) with varying degrees of coating. Diverse studies were performed to analyze the toxicity of NPs against cells in a dose- and time-dependent manner. To ensure the decreased toxicity of the produced SiO2/ZnO NPs, cytotoxicity in membrane damage and/or intracellular reactive oxygen species (ROS) were assessed by employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lactate dehydrogenase, 2',7'-dichlorofluorescin, and lipid peroxide estimations. The cores of ZnO NPs exhibited cytotoxicity over time, regardless of shell thickness. Nevertheless, the thicker SiO2/ZnO NPs revealed reduced enzyme leakage, decreased peroxide production, and less oxidative stress than their bare ZnO NPs or thinner SiO2/ZnO NPs. Therefore, thicker SiO2/ZnO NPs moderated the toxicity of ZnO NPs by restricting free radical formation and the release of zinc ions, and decreasing surface contact with cells. Keywords: zinc oxide, silica coating, photostability, human dermal fibroblast, membrane damage, oxidative stressRamasamy MDas MAn SSAYi DKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3707-3718 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ramasamy M Das M An SSA Yi DK Role of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells
description	Mohankandhasamy Ramasamy,1 Minakshi Das,1 Seong Soo A An,1 Dong Kee Yi2 1Division of Bionanotechnology, Gachon University, Seongnam, 2Department of Chemistry, Myongji University, Yongin, South Korea Abstract: The wide-scale applications of zinc oxide (ZnO) nanoparticles (NPs) in photocatalysts, gas sensors, and cosmetics may cause toxicity to humans and environments. Therefore, the aim of the present study was to reduce the toxicity of ZnO NPs by coating them with a silica (SiO2) layer, which could be used in human applications, such as cosmetic preparations. The sol–gel method was used to synthesize core ZnO with SiO2-shelled NPs (SiO2/ZnO NPs) with varying degrees of coating. Diverse studies were performed to analyze the toxicity of NPs against cells in a dose- and time-dependent manner. To ensure the decreased toxicity of the produced SiO2/ZnO NPs, cytotoxicity in membrane damage and/or intracellular reactive oxygen species (ROS) were assessed by employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lactate dehydrogenase, 2',7'-dichlorofluorescin, and lipid peroxide estimations. The cores of ZnO NPs exhibited cytotoxicity over time, regardless of shell thickness. Nevertheless, the thicker SiO2/ZnO NPs revealed reduced enzyme leakage, decreased peroxide production, and less oxidative stress than their bare ZnO NPs or thinner SiO2/ZnO NPs. Therefore, thicker SiO2/ZnO NPs moderated the toxicity of ZnO NPs by restricting free radical formation and the release of zinc ions, and decreasing surface contact with cells. Keywords: zinc oxide, silica coating, photostability, human dermal fibroblast, membrane damage, oxidative stress
format	article
author	Ramasamy M Das M An SSA Yi DK
author_facet	Ramasamy M Das M An SSA Yi DK
author_sort	Ramasamy M
title	Role of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells
title_short	Role of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells
title_full	Role of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells
title_fullStr	Role of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells
title_full_unstemmed	Role of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells
title_sort	role of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/0c9a830d96154617958f13342b0f9db0
work_keys_str_mv	AT ramasamym roleofsurfacemodificationinzincoxidenanoparticlesanditstoxicityassessmenttowardhumandermalfibroblastcells AT dasm roleofsurfacemodificationinzincoxidenanoparticlesanditstoxicityassessmenttowardhumandermalfibroblastcells AT anssa roleofsurfacemodificationinzincoxidenanoparticlesanditstoxicityassessmenttowardhumandermalfibroblastcells AT yidk roleofsurfacemodificationinzincoxidenanoparticlesanditstoxicityassessmenttowardhumandermalfibroblastcells
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Role of surface modification in zinc oxide nanoparticles and its toxicity assessment toward human dermal fibroblast cells

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