Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants

Abstract Forests are under threat from pests, pathogens, and changing climate. A major forest pathogen worldwide is the hemibiotroph Dothistroma septosporum, which causes dothistroma needle blight (DNB) of pines. While D. septosporum uses effector proteins to facilitate host infection, it is current...

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Autores principales:	Lukas Hunziker, Mariana Tarallo, Keiko Gough, Melissa Guo, Cathy Hargreaves, Trevor S. Loo, Rebecca L. McDougal, Carl H. Mesarich, Rosie E. Bradshaw
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ca29a47e8fa404da18d6849a8ab117f
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spelling	oai:doaj.org-article:0ca29a47e8fa404da18d6849a8ab117f2021-12-02T18:37:10ZApoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants10.1038/s41598-021-99415-52045-2322https://doaj.org/article/0ca29a47e8fa404da18d6849a8ab117f2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99415-5https://doaj.org/toc/2045-2322Abstract Forests are under threat from pests, pathogens, and changing climate. A major forest pathogen worldwide is the hemibiotroph Dothistroma septosporum, which causes dothistroma needle blight (DNB) of pines. While D. septosporum uses effector proteins to facilitate host infection, it is currently unclear whether any of these effectors are recognised by immune receptors to activate the host immune system. Such information is needed to identify and select disease resistance against D. septosporum in pines. We predicted and investigated apoplastic D. septosporum candidate effectors (DsCEs) using bioinformatics and plant-based experiments. We discovered DsCEs that trigger cell death in the angiosperm Nicotiana spp., indicative of a hypersensitive defence response and suggesting their recognition by immune receptors in non-host plants. In a first for foliar forest pathogens, we developed a novel protein infiltration method to show that tissue-cultured pine shoots can respond with a cell death response to a DsCE, as well as to a reference cell death-inducing protein. The conservation of responses across plant taxa suggests that knowledge of pathogen–angiosperm interactions may also be relevant to pathogen–gymnosperm interactions. These results contribute to our understanding of forest pathogens and may ultimately provide clues to disease immunity in both commercial and natural forests.Lukas HunzikerMariana TaralloKeiko GoughMelissa GuoCathy HargreavesTrevor S. LooRebecca L. McDougalCarl H. MesarichRosie E. BradshawNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lukas Hunziker Mariana Tarallo Keiko Gough Melissa Guo Cathy Hargreaves Trevor S. Loo Rebecca L. McDougal Carl H. Mesarich Rosie E. Bradshaw Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants
description	Abstract Forests are under threat from pests, pathogens, and changing climate. A major forest pathogen worldwide is the hemibiotroph Dothistroma septosporum, which causes dothistroma needle blight (DNB) of pines. While D. septosporum uses effector proteins to facilitate host infection, it is currently unclear whether any of these effectors are recognised by immune receptors to activate the host immune system. Such information is needed to identify and select disease resistance against D. septosporum in pines. We predicted and investigated apoplastic D. septosporum candidate effectors (DsCEs) using bioinformatics and plant-based experiments. We discovered DsCEs that trigger cell death in the angiosperm Nicotiana spp., indicative of a hypersensitive defence response and suggesting their recognition by immune receptors in non-host plants. In a first for foliar forest pathogens, we developed a novel protein infiltration method to show that tissue-cultured pine shoots can respond with a cell death response to a DsCE, as well as to a reference cell death-inducing protein. The conservation of responses across plant taxa suggests that knowledge of pathogen–angiosperm interactions may also be relevant to pathogen–gymnosperm interactions. These results contribute to our understanding of forest pathogens and may ultimately provide clues to disease immunity in both commercial and natural forests.
format	article
author	Lukas Hunziker Mariana Tarallo Keiko Gough Melissa Guo Cathy Hargreaves Trevor S. Loo Rebecca L. McDougal Carl H. Mesarich Rosie E. Bradshaw
author_facet	Lukas Hunziker Mariana Tarallo Keiko Gough Melissa Guo Cathy Hargreaves Trevor S. Loo Rebecca L. McDougal Carl H. Mesarich Rosie E. Bradshaw
author_sort	Lukas Hunziker
title	Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants
title_short	Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants
title_full	Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants
title_fullStr	Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants
title_full_unstemmed	Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants
title_sort	apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0ca29a47e8fa404da18d6849a8ab117f
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Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants

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