Culture independent assessment of human milk microbial community in lactational mastitis

Abstract Breastfeeding undoubtedly provides important benefits to the mother-infant dyad and should be encouraged. Mastitis, one of the common but major cause of premature weaning among lactating women, is an inflammation of connective tissue within the mammary gland. This study reports the influenc...

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Autores principales:	Shriram H. Patel, Yati H. Vaidya, Reena J. Patel, Ramesh J. Pandit, Chaitanya G. Joshi, Anju P. Kunjadiya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ca725a8e1f34a44827dc98af1069ae1
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spelling	oai:doaj.org-article:0ca725a8e1f34a44827dc98af1069ae12021-12-02T16:06:37ZCulture independent assessment of human milk microbial community in lactational mastitis10.1038/s41598-017-08451-72045-2322https://doaj.org/article/0ca725a8e1f34a44827dc98af1069ae12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08451-7https://doaj.org/toc/2045-2322Abstract Breastfeeding undoubtedly provides important benefits to the mother-infant dyad and should be encouraged. Mastitis, one of the common but major cause of premature weaning among lactating women, is an inflammation of connective tissue within the mammary gland. This study reports the influence of mastitis on human milk microbiota by utilizing 16 S rRNA gene sequencing approach. We sampled and sequenced microbiome from 50 human milk samples, including 16 subacute mastitis (SAM), 16 acute mastitis (AM) and 18 healthy-controls. Compared to controls, SAM and AM microbiota were quite distinct and drastically reduced. Genera including, Aeromonas, Staphylococcus, Ralstonia, Klebsiella, Serratia, Enterococcus and Pseudomonas were significantly enriched in SAM and AM samples, while Acinetobacter, Ruminococcus, Clostridium, Faecalibacterium and Eubacterium were consistently depleted. Further analysis of our samples revealed positive aerotolerant odds ratio, indicating dramatic depletion of obligate anaerobes and enrichment of aerotolerant bacteria during the course of mastitis. In addition, predicted functional metagenomics identified several gene pathways related to bacterial proliferation and colonization (e.g. two-component system, bacterial secretion system and motility proteins) in SAM and AM samples. In conclusion, our study confirmed previous hypothesis that mastitis women have lower microbial diversity, increased abundance of opportunistic pathogens and depletion of commensal obligate anaerobes.Shriram H. PatelYati H. VaidyaReena J. PatelRamesh J. PanditChaitanya G. JoshiAnju P. KunjadiyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shriram H. Patel Yati H. Vaidya Reena J. Patel Ramesh J. Pandit Chaitanya G. Joshi Anju P. Kunjadiya Culture independent assessment of human milk microbial community in lactational mastitis
description	Abstract Breastfeeding undoubtedly provides important benefits to the mother-infant dyad and should be encouraged. Mastitis, one of the common but major cause of premature weaning among lactating women, is an inflammation of connective tissue within the mammary gland. This study reports the influence of mastitis on human milk microbiota by utilizing 16 S rRNA gene sequencing approach. We sampled and sequenced microbiome from 50 human milk samples, including 16 subacute mastitis (SAM), 16 acute mastitis (AM) and 18 healthy-controls. Compared to controls, SAM and AM microbiota were quite distinct and drastically reduced. Genera including, Aeromonas, Staphylococcus, Ralstonia, Klebsiella, Serratia, Enterococcus and Pseudomonas were significantly enriched in SAM and AM samples, while Acinetobacter, Ruminococcus, Clostridium, Faecalibacterium and Eubacterium were consistently depleted. Further analysis of our samples revealed positive aerotolerant odds ratio, indicating dramatic depletion of obligate anaerobes and enrichment of aerotolerant bacteria during the course of mastitis. In addition, predicted functional metagenomics identified several gene pathways related to bacterial proliferation and colonization (e.g. two-component system, bacterial secretion system and motility proteins) in SAM and AM samples. In conclusion, our study confirmed previous hypothesis that mastitis women have lower microbial diversity, increased abundance of opportunistic pathogens and depletion of commensal obligate anaerobes.
format	article
author	Shriram H. Patel Yati H. Vaidya Reena J. Patel Ramesh J. Pandit Chaitanya G. Joshi Anju P. Kunjadiya
author_facet	Shriram H. Patel Yati H. Vaidya Reena J. Patel Ramesh J. Pandit Chaitanya G. Joshi Anju P. Kunjadiya
author_sort	Shriram H. Patel
title	Culture independent assessment of human milk microbial community in lactational mastitis
title_short	Culture independent assessment of human milk microbial community in lactational mastitis
title_full	Culture independent assessment of human milk microbial community in lactational mastitis
title_fullStr	Culture independent assessment of human milk microbial community in lactational mastitis
title_full_unstemmed	Culture independent assessment of human milk microbial community in lactational mastitis
title_sort	culture independent assessment of human milk microbial community in lactational mastitis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0ca725a8e1f34a44827dc98af1069ae1
work_keys_str_mv	AT shriramhpatel cultureindependentassessmentofhumanmilkmicrobialcommunityinlactationalmastitis AT yatihvaidya cultureindependentassessmentofhumanmilkmicrobialcommunityinlactationalmastitis AT reenajpatel cultureindependentassessmentofhumanmilkmicrobialcommunityinlactationalmastitis AT rameshjpandit cultureindependentassessmentofhumanmilkmicrobialcommunityinlactationalmastitis AT chaitanyagjoshi cultureindependentassessmentofhumanmilkmicrobialcommunityinlactationalmastitis AT anjupkunjadiya cultureindependentassessmentofhumanmilkmicrobialcommunityinlactationalmastitis
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Culture independent assessment of human milk microbial community in lactational mastitis

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