Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area

Abstract Background The dysregulation of B cell activation is prevalent during naturally acquired immunity against malaria. Osteopontin (OPN), a protein produced by various cells including B cells, is a phosphorylated glycoprotein that participates in immune regulation and has been suggested to be i...

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Autores principales:	Susanne E Mortazavi, Allan Lugaajju, Mark Kaddumukasa, Muyideen Kolapo Tijani, Fred Kironde, Kristina E M Persson
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Osteopontin Malaria Plasmodium falciparum B cell BAFF Immunity Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0caa38b4d0304c188464c9729e59d9c0
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spelling	oai:doaj.org-article:0caa38b4d0304c188464c9729e59d9c02021-11-07T12:08:56ZOsteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area10.1186/s12866-021-02368-y1471-2180https://doaj.org/article/0caa38b4d0304c188464c9729e59d9c02021-11-01T00:00:00Zhttps://doi.org/10.1186/s12866-021-02368-yhttps://doaj.org/toc/1471-2180Abstract Background The dysregulation of B cell activation is prevalent during naturally acquired immunity against malaria. Osteopontin (OPN), a protein produced by various cells including B cells, is a phosphorylated glycoprotein that participates in immune regulation and has been suggested to be involved in the immune response against malaria. Here we studied the longitudinal concentrations of OPN in infants and their mothers living in Uganda, and how OPN concentrations correlated with B cell subsets specific for P. falciparum and B cell activating factor (BAFF). We also investigated the direct effect of OPN on P. falciparum in vitro. Results The OPN concentration was higher in the infants compared to the mothers, and OPN concentration in infants decreased from birth until 9 months. OPN concentration in infants during 9 months were independent of OPN concentrations in corresponding mothers. OPN concentrations in infants were inversely correlated with total atypical memory B cells (MBCs) as well as P. falciparum-specific atypical MBCs. There was a positive correlation between OPN and BAFF concentrations in both mothers and infants. When OPN was added to P. falciparum cultured in vitro, parasitemia was unaffected regardless of OPN concentration. Conclusions The concentrations of OPN in infants were higher and independent of the OPN concentrations in corresponding mothers. In vitro, OPN does not have a direct effect on P. falciparum growth. Our correlation analysis results suggest that OPN could have a role in the B cell immune response and acquisition of natural immunity against malaria.Susanne E MortazaviAllan LugaajjuMark KaddumukasaMuyideen Kolapo TijaniFred KirondeKristina E M PerssonBMCarticleOsteopontinMalariaPlasmodium falciparumB cellBAFFImmunityMicrobiologyQR1-502ENBMC Microbiology, Vol 21, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteopontin Malaria Plasmodium falciparum B cell BAFF Immunity Microbiology QR1-502
spellingShingle	Osteopontin Malaria Plasmodium falciparum B cell BAFF Immunity Microbiology QR1-502 Susanne E Mortazavi Allan Lugaajju Mark Kaddumukasa Muyideen Kolapo Tijani Fred Kironde Kristina E M Persson Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area
description	Abstract Background The dysregulation of B cell activation is prevalent during naturally acquired immunity against malaria. Osteopontin (OPN), a protein produced by various cells including B cells, is a phosphorylated glycoprotein that participates in immune regulation and has been suggested to be involved in the immune response against malaria. Here we studied the longitudinal concentrations of OPN in infants and their mothers living in Uganda, and how OPN concentrations correlated with B cell subsets specific for P. falciparum and B cell activating factor (BAFF). We also investigated the direct effect of OPN on P. falciparum in vitro. Results The OPN concentration was higher in the infants compared to the mothers, and OPN concentration in infants decreased from birth until 9 months. OPN concentration in infants during 9 months were independent of OPN concentrations in corresponding mothers. OPN concentrations in infants were inversely correlated with total atypical memory B cells (MBCs) as well as P. falciparum-specific atypical MBCs. There was a positive correlation between OPN and BAFF concentrations in both mothers and infants. When OPN was added to P. falciparum cultured in vitro, parasitemia was unaffected regardless of OPN concentration. Conclusions The concentrations of OPN in infants were higher and independent of the OPN concentrations in corresponding mothers. In vitro, OPN does not have a direct effect on P. falciparum growth. Our correlation analysis results suggest that OPN could have a role in the B cell immune response and acquisition of natural immunity against malaria.
format	article
author	Susanne E Mortazavi Allan Lugaajju Mark Kaddumukasa Muyideen Kolapo Tijani Fred Kironde Kristina E M Persson
author_facet	Susanne E Mortazavi Allan Lugaajju Mark Kaddumukasa Muyideen Kolapo Tijani Fred Kironde Kristina E M Persson
author_sort	Susanne E Mortazavi
title	Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area
title_short	Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area
title_full	Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area
title_fullStr	Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area
title_full_unstemmed	Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area
title_sort	osteopontin and malaria: no direct effect on parasite growth, but correlation with p. falciparum-specific b cells and baff in a malaria endemic area
publisher	BMC
publishDate	2021
url	https://doaj.org/article/0caa38b4d0304c188464c9729e59d9c0
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Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area

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