The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats

Abstract This experiment aimed to investigate the effect of dietary Nigella sativa on the cell-mediated immune response. Eighteen male Wistar rats were divided equally into a control group and treated groups that received black seeds at rates of 30 and 50 g/kg in the diet (Sa30 and Sa50 groups, resp...

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Autores principales:	Hany Salah Mahmoud, Amani A. Almallah, Heba Nageh Gad EL-Hak, Tahany Saleh Aldayel, Heba M. A. Abdelrazek, Howayda E. Khaled
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0caa5e77dc914d4fb8aeb8139db49d92
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spelling	oai:doaj.org-article:0caa5e77dc914d4fb8aeb8139db49d922021-12-02T14:17:19ZThe effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats10.1038/s41598-021-86721-12045-2322https://doaj.org/article/0caa5e77dc914d4fb8aeb8139db49d922021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86721-1https://doaj.org/toc/2045-2322Abstract This experiment aimed to investigate the effect of dietary Nigella sativa on the cell-mediated immune response. Eighteen male Wistar rats were divided equally into a control group and treated groups that received black seeds at rates of 30 and 50 g/kg in the diet (Sa30 and Sa50 groups, respectively, for 30 days. The weight gain, feed intake, feed conversion ratio (FCR), and cell-mediated immune response were monitored after the injection of 0.1 mL of 10% phytohemagglutinin (PHA). The intumesce index, serum total antioxidant capacity (TAC), catalase (CAT), interleukin-12 (IL-12), gamma interferon (γ-IF) and tumor necrosis factor alpha (TNF-α) were determined. Histopathological examination and an immunohistochemistry analysis of splenic caspase-3 and CD8 were performed. Nigella sativa significantly improved the weight gain and FCR. Intumesce index of Sa50 group was significantly increased. Nigella sativa significantly increased TAC, CAT, IL-12, γ-IF and TNF-α. A histological examination of PHA-stimulated foot pads showed increased leukocyte infiltration and edema in a dose-dependent pattern. Splenic caspase-3 and CD8 showed significant decreases and increases, respectively, in the Sa30 and Sa50 groups. The results indicate that Nigella sativa seeds exhibit immunostimulatory function through their antioxidant potential, induction of cytokine production, promotion of CD8 expression and reduction of splenic apoptosis.Hany Salah MahmoudAmani A. AlmallahHeba Nageh Gad EL-HakTahany Saleh AldayelHeba M. A. AbdelrazekHowayda E. KhaledNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hany Salah Mahmoud Amani A. Almallah Heba Nageh Gad EL-Hak Tahany Saleh Aldayel Heba M. A. Abdelrazek Howayda E. Khaled The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats
description	Abstract This experiment aimed to investigate the effect of dietary Nigella sativa on the cell-mediated immune response. Eighteen male Wistar rats were divided equally into a control group and treated groups that received black seeds at rates of 30 and 50 g/kg in the diet (Sa30 and Sa50 groups, respectively, for 30 days. The weight gain, feed intake, feed conversion ratio (FCR), and cell-mediated immune response were monitored after the injection of 0.1 mL of 10% phytohemagglutinin (PHA). The intumesce index, serum total antioxidant capacity (TAC), catalase (CAT), interleukin-12 (IL-12), gamma interferon (γ-IF) and tumor necrosis factor alpha (TNF-α) were determined. Histopathological examination and an immunohistochemistry analysis of splenic caspase-3 and CD8 were performed. Nigella sativa significantly improved the weight gain and FCR. Intumesce index of Sa50 group was significantly increased. Nigella sativa significantly increased TAC, CAT, IL-12, γ-IF and TNF-α. A histological examination of PHA-stimulated foot pads showed increased leukocyte infiltration and edema in a dose-dependent pattern. Splenic caspase-3 and CD8 showed significant decreases and increases, respectively, in the Sa30 and Sa50 groups. The results indicate that Nigella sativa seeds exhibit immunostimulatory function through their antioxidant potential, induction of cytokine production, promotion of CD8 expression and reduction of splenic apoptosis.
format	article
author	Hany Salah Mahmoud Amani A. Almallah Heba Nageh Gad EL-Hak Tahany Saleh Aldayel Heba M. A. Abdelrazek Howayda E. Khaled
author_facet	Hany Salah Mahmoud Amani A. Almallah Heba Nageh Gad EL-Hak Tahany Saleh Aldayel Heba M. A. Abdelrazek Howayda E. Khaled
author_sort	Hany Salah Mahmoud
title	The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats
title_short	The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats
title_full	The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats
title_fullStr	The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats
title_full_unstemmed	The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats
title_sort	effect of dietary supplementation with nigella sativa (black seeds) mediates immunological function in male wistar rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0caa5e77dc914d4fb8aeb8139db49d92
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The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats

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