In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

Aml I Mekkawy,1 Mohamed A El-Mokhtar,2 Nivien A Nafady,3 Naeima Yousef,3 Mostafa A Hamad,4 Sohair M El-Shanawany,5 Ehsan H Ibrahim,5 Mahmoud Elsabahy5–8 1Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag, 2Department of Microbiology and Immuno...

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Autores principales:	Mekkawy AI, El-Mokhtar MA, Nafady NA, Yousef N, Hamad MA, El-Shanawany SM, Ibrahim EH, Elsabahy M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Silver nanoparticles green synthesis coating agents hydrogel wound healing antibacterial activity. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0cabccc519df4e31a894b6ae7e5778b8
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spelling	oai:doaj.org-article:0cabccc519df4e31a894b6ae7e5778b82021-12-02T07:13:42ZIn vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels1178-2013https://doaj.org/article/0cabccc519df4e31a894b6ae7e5778b82017-01-01T00:00:00Zhttps://www.dovepress.com/in-vitro-and-in-vivo-evaluation-of-biologically-synthesized-silver-nan-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Aml I Mekkawy,1 Mohamed A El-Mokhtar,2 Nivien A Nafady,3 Naeima Yousef,3 Mostafa A Hamad,4 Sohair M El-Shanawany,5 Ehsan H Ibrahim,5 Mahmoud Elsabahy5–8 1Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag, 2Department of Microbiology and Immunology, Faculty of Medicine, 3Department of Botany and Microbiology, Faculty of Science, 4Department of Surgery, Faculty of Medicine, 5Department of Pharmaceutics, Faculty of Pharmacy, 6Assiut International Center of Nanomedicine, Al-Rajhi Liver Hospital, Assiut University, Assiut, Egypt; 7Laboratory for Synthetic-Biologic Interactions, Department of Chemistry, Texas A&M University, College Station, TX, USA; 8Misr University for Science and Technology, 6th of October, Egypt Abstract: In the present study, silver nanoparticles (AgNPs) were synthesized via biological reduction of silver nitrate using extract of the fungus Fusarium verticillioides (green chemistry principle). The synthesized nanoparticles were spherical and homogenous in size. AgNPs were coated with polyethylene glycol (PEG) 6000, sodium dodecyl sulfate (SDS), and β-cyclodextrin (β-CD). The averaged diameters of AgNPs were 19.2±3.6, 13±4, 14±4.4, and 15.7±4.8 nm, for PEG-, SDS-, and ß-CD-coated and uncoated AgNPs, respectively. PEG-coated AgNPs showed greater stability as indicated by a decreased sedimentation rate of particles in their water dispersions. The antibacterial activities of different AgNPs dispersions were investigated against Gram-positive bacteria (methicillin-sensitive and methicillin-resistant Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) by determination of the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). MIC and MBC values were in the range of 0.93–7.5 and 3.75–15 µg/mL, respectively, which were superior to the reported values in literature. AgNPs-loaded hydrogels were prepared from the coated-AgNPs dispersions using several gelling agents (sodium carboxymethyl cellulose [Na CMC], sodium alginate, hydroxypropylmethyl cellulose, Pluronic F-127, and chitosan). The prepared formulations were evaluated for their viscosity, spreadability, in vitro drug release, and antibacterial activity, and the combined effect of the type of surface coating and the polymers utilized to form the gel was studied. The in vivo wound-healing activity and antibacterial efficacy of Na CMC hydrogel loaded with PEG-coated AgNPs in comparison to the commercially available silver sulfadiazine cream (Dermazin®) were evaluated. Superior antibacterial activity and wound-healing capability, with normal skin appearance and hair growth, were demonstrated for the hydrogel formulations, as compared to the silver sulfadiazine cream. Histological examination of the treated skin was performed using light microscopy, whereas the location of AgNPs in the skin epidermal layers was visualized using transmission electron microscopy. Keywords: silver nanoparticles, green synthesis, coating agents, hydrogel, wound healing, antibacterial activityMekkawy AIEl-Mokhtar MANafady NAYousef NHamad MAEl-Shanawany SMIbrahim EHElsabahy MDove Medical PressarticleSilver nanoparticlesgreen synthesiscoating agentshydrogelwound healingantibacterial activity.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 759-777 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silver nanoparticles green synthesis coating agents hydrogel wound healing antibacterial activity. Medicine (General) R5-920
spellingShingle	Silver nanoparticles green synthesis coating agents hydrogel wound healing antibacterial activity. Medicine (General) R5-920 Mekkawy AI El-Mokhtar MA Nafady NA Yousef N Hamad MA El-Shanawany SM Ibrahim EH Elsabahy M In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels
description	Aml I Mekkawy,1 Mohamed A El-Mokhtar,2 Nivien A Nafady,3 Naeima Yousef,3 Mostafa A Hamad,4 Sohair M El-Shanawany,5 Ehsan H Ibrahim,5 Mahmoud Elsabahy5–8 1Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag, 2Department of Microbiology and Immunology, Faculty of Medicine, 3Department of Botany and Microbiology, Faculty of Science, 4Department of Surgery, Faculty of Medicine, 5Department of Pharmaceutics, Faculty of Pharmacy, 6Assiut International Center of Nanomedicine, Al-Rajhi Liver Hospital, Assiut University, Assiut, Egypt; 7Laboratory for Synthetic-Biologic Interactions, Department of Chemistry, Texas A&M University, College Station, TX, USA; 8Misr University for Science and Technology, 6th of October, Egypt Abstract: In the present study, silver nanoparticles (AgNPs) were synthesized via biological reduction of silver nitrate using extract of the fungus Fusarium verticillioides (green chemistry principle). The synthesized nanoparticles were spherical and homogenous in size. AgNPs were coated with polyethylene glycol (PEG) 6000, sodium dodecyl sulfate (SDS), and β-cyclodextrin (β-CD). The averaged diameters of AgNPs were 19.2±3.6, 13±4, 14±4.4, and 15.7±4.8 nm, for PEG-, SDS-, and ß-CD-coated and uncoated AgNPs, respectively. PEG-coated AgNPs showed greater stability as indicated by a decreased sedimentation rate of particles in their water dispersions. The antibacterial activities of different AgNPs dispersions were investigated against Gram-positive bacteria (methicillin-sensitive and methicillin-resistant Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) by determination of the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). MIC and MBC values were in the range of 0.93–7.5 and 3.75–15 µg/mL, respectively, which were superior to the reported values in literature. AgNPs-loaded hydrogels were prepared from the coated-AgNPs dispersions using several gelling agents (sodium carboxymethyl cellulose [Na CMC], sodium alginate, hydroxypropylmethyl cellulose, Pluronic F-127, and chitosan). The prepared formulations were evaluated for their viscosity, spreadability, in vitro drug release, and antibacterial activity, and the combined effect of the type of surface coating and the polymers utilized to form the gel was studied. The in vivo wound-healing activity and antibacterial efficacy of Na CMC hydrogel loaded with PEG-coated AgNPs in comparison to the commercially available silver sulfadiazine cream (Dermazin®) were evaluated. Superior antibacterial activity and wound-healing capability, with normal skin appearance and hair growth, were demonstrated for the hydrogel formulations, as compared to the silver sulfadiazine cream. Histological examination of the treated skin was performed using light microscopy, whereas the location of AgNPs in the skin epidermal layers was visualized using transmission electron microscopy. Keywords: silver nanoparticles, green synthesis, coating agents, hydrogel, wound healing, antibacterial activity
format	article
author	Mekkawy AI El-Mokhtar MA Nafady NA Yousef N Hamad MA El-Shanawany SM Ibrahim EH Elsabahy M
author_facet	Mekkawy AI El-Mokhtar MA Nafady NA Yousef N Hamad MA El-Shanawany SM Ibrahim EH Elsabahy M
author_sort	Mekkawy AI
title	In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels
title_short	In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels
title_full	In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels
title_fullStr	In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels
title_full_unstemmed	In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels
title_sort	in vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/0cabccc519df4e31a894b6ae7e5778b8
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In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

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