Local photoreceptor cell death differences in the murine model of retinal detachment

Abstract To investigate local cell death differences in the attached and detached retina at different regions in a murine experimental retinal detachment model. Subretinal injection of sodium hyaluronate was performed in eight-week-old C57BL/6J mice. Retinal regions of interest were defined in relat...

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Autores principales:	Daniel E. Maidana, Lucia Gonzalez-Buendia, Joan W. Miller, Demetrios G. Vavvas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cacc61aa82040a9a5fc0c42603c839a
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spelling	oai:doaj.org-article:0cacc61aa82040a9a5fc0c42603c839a2021-12-02T17:26:49ZLocal photoreceptor cell death differences in the murine model of retinal detachment10.1038/s41598-021-97947-42045-2322https://doaj.org/article/0cacc61aa82040a9a5fc0c42603c839a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97947-4https://doaj.org/toc/2045-2322Abstract To investigate local cell death differences in the attached and detached retina at different regions in a murine experimental retinal detachment model. Subretinal injection of sodium hyaluronate was performed in eight-week-old C57BL/6J mice. Retinal regions of interest were defined in relation to their distance from the peak of the retinal detachment, as follows: (1) attached central; (2) attached paracentral; (3) detached apex; and (4) detached base. At day 0, the outer nuclear layer cell count for the attached central, attached paracentral, detached apex, and detached base was 1247.60 ± 64.62, 1157.80 ± 163.33, 1264.00 ± 150.7, and 1013.80 ± 67.16 cells, respectively. There were significant differences between the detached base vs. attached central, and between detached base vs. detached apex at day 0. The detached apex region displayed a significant and progressive cell count reduction from day 0 to 14. In contrast, the detached base region did not show progressive retinal degeneration in this model. Moreover, only the detached apex region had a significant and progressive cell death rate compared to baseline. Immediate confounding changes with dramatic differences in cell death rates are present across regions of the detached retina. We speculate that mechanical and regional differences in the bullous detached retina can modify the rate of cell death in this model.Daniel E. MaidanaLucia Gonzalez-BuendiaJoan W. MillerDemetrios G. VavvasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel E. Maidana Lucia Gonzalez-Buendia Joan W. Miller Demetrios G. Vavvas Local photoreceptor cell death differences in the murine model of retinal detachment
description	Abstract To investigate local cell death differences in the attached and detached retina at different regions in a murine experimental retinal detachment model. Subretinal injection of sodium hyaluronate was performed in eight-week-old C57BL/6J mice. Retinal regions of interest were defined in relation to their distance from the peak of the retinal detachment, as follows: (1) attached central; (2) attached paracentral; (3) detached apex; and (4) detached base. At day 0, the outer nuclear layer cell count for the attached central, attached paracentral, detached apex, and detached base was 1247.60 ± 64.62, 1157.80 ± 163.33, 1264.00 ± 150.7, and 1013.80 ± 67.16 cells, respectively. There were significant differences between the detached base vs. attached central, and between detached base vs. detached apex at day 0. The detached apex region displayed a significant and progressive cell count reduction from day 0 to 14. In contrast, the detached base region did not show progressive retinal degeneration in this model. Moreover, only the detached apex region had a significant and progressive cell death rate compared to baseline. Immediate confounding changes with dramatic differences in cell death rates are present across regions of the detached retina. We speculate that mechanical and regional differences in the bullous detached retina can modify the rate of cell death in this model.
format	article
author	Daniel E. Maidana Lucia Gonzalez-Buendia Joan W. Miller Demetrios G. Vavvas
author_facet	Daniel E. Maidana Lucia Gonzalez-Buendia Joan W. Miller Demetrios G. Vavvas
author_sort	Daniel E. Maidana
title	Local photoreceptor cell death differences in the murine model of retinal detachment
title_short	Local photoreceptor cell death differences in the murine model of retinal detachment
title_full	Local photoreceptor cell death differences in the murine model of retinal detachment
title_fullStr	Local photoreceptor cell death differences in the murine model of retinal detachment
title_full_unstemmed	Local photoreceptor cell death differences in the murine model of retinal detachment
title_sort	local photoreceptor cell death differences in the murine model of retinal detachment
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0cacc61aa82040a9a5fc0c42603c839a
work_keys_str_mv	AT danielemaidana localphotoreceptorcelldeathdifferencesinthemurinemodelofretinaldetachment AT luciagonzalezbuendia localphotoreceptorcelldeathdifferencesinthemurinemodelofretinaldetachment AT joanwmiller localphotoreceptorcelldeathdifferencesinthemurinemodelofretinaldetachment AT demetriosgvavvas localphotoreceptorcelldeathdifferencesinthemurinemodelofretinaldetachment
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Local photoreceptor cell death differences in the murine model of retinal detachment

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