DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.

Morbidity and mortality of prostate cancer (PCa) have increased in recent years worldwide. Currently existing methods for diagnosis and treatment do not make the situation improve, especially for hormone refractory prostate cancer (HRPC). The lack of molecular probes for PCa hindered the early diagn...

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Autores principales:	Yuanyuan Wang, Yun Luo, Tao Bing, Zheng Chen, Minhua Lu, Nan Zhang, Dihua Shangguan, Xin Gao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cb362c008bf4e1ba3174b1ec5983af0
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spelling	oai:doaj.org-article:0cb362c008bf4e1ba3174b1ec5983af02021-11-18T08:14:37ZDNA aptamer evolved by cell-SELEX for recognition of prostate cancer.1932-620310.1371/journal.pone.0100243https://doaj.org/article/0cb362c008bf4e1ba3174b1ec5983af02014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24956390/?tool=EBIhttps://doaj.org/toc/1932-6203Morbidity and mortality of prostate cancer (PCa) have increased in recent years worldwide. Currently existing methods for diagnosis and treatment do not make the situation improve, especially for hormone refractory prostate cancer (HRPC). The lack of molecular probes for PCa hindered the early diagnosis of metastasis and accurate staging for PCa. In this work, we have developed a new aptamer probe Wy-5a against PCa cell line PC-3 by cell-SELEX technique. Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines. The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa. The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa.Yuanyuan WangYun LuoTao BingZheng ChenMinhua LuNan ZhangDihua ShangguanXin GaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100243 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuanyuan Wang Yun Luo Tao Bing Zheng Chen Minhua Lu Nan Zhang Dihua Shangguan Xin Gao DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.
description	Morbidity and mortality of prostate cancer (PCa) have increased in recent years worldwide. Currently existing methods for diagnosis and treatment do not make the situation improve, especially for hormone refractory prostate cancer (HRPC). The lack of molecular probes for PCa hindered the early diagnosis of metastasis and accurate staging for PCa. In this work, we have developed a new aptamer probe Wy-5a against PCa cell line PC-3 by cell-SELEX technique. Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines. The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa. The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa.
format	article
author	Yuanyuan Wang Yun Luo Tao Bing Zheng Chen Minhua Lu Nan Zhang Dihua Shangguan Xin Gao
author_facet	Yuanyuan Wang Yun Luo Tao Bing Zheng Chen Minhua Lu Nan Zhang Dihua Shangguan Xin Gao
author_sort	Yuanyuan Wang
title	DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.
title_short	DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.
title_full	DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.
title_fullStr	DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.
title_full_unstemmed	DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.
title_sort	dna aptamer evolved by cell-selex for recognition of prostate cancer.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0cb362c008bf4e1ba3174b1ec5983af0
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DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.

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