Stimulatory interactions between human coronary smooth muscle cells and dendritic cells.

Despite inflammatory and immune mechanisms participating to atherogenesis and dendritic cells (DCs) driving immune and non-immune tissue injury response, the interactions between DCs and vascular smooth muscle cells (VSMCs) possibly relevant to vascular pathology including atherogenesis are still un...

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Autores principales:	Sara Paccosi, Claudia Musilli, Roberto Caporale, Anna Maria Grazia Gelli, Daniele Guasti, Ann Maria Clemente, Maria Gabriella Torcia, Amelia Filippelli, Paolo Romagnoli, Astrid Parenti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cb6ca6d1bbb4e47939d013afd5a52b3
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spelling	oai:doaj.org-article:0cb6ca6d1bbb4e47939d013afd5a52b32021-11-18T08:15:35ZStimulatory interactions between human coronary smooth muscle cells and dendritic cells.1932-620310.1371/journal.pone.0099652https://doaj.org/article/0cb6ca6d1bbb4e47939d013afd5a52b32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24932497/?tool=EBIhttps://doaj.org/toc/1932-6203Despite inflammatory and immune mechanisms participating to atherogenesis and dendritic cells (DCs) driving immune and non-immune tissue injury response, the interactions between DCs and vascular smooth muscle cells (VSMCs) possibly relevant to vascular pathology including atherogenesis are still unclear. To address this issue, immature DCs (iDCs) generated from CD14+ cells isolated from healthy donors were matured either with cytokines (mDCs), or co-cultured (ccDCs) with human coronary artery VSMCs (CASMCs) using transwell chambers. Co-culture induced DC immunophenotypical and functional maturation similar to cytokines, as demonstrated by flow cytometry and mixed lymphocyte reaction. In turn, factors from mDCs and ccDCs induced CASMC migration. MCP-1 and TNFα, secreted from DCs, and IL-6 and MCP-1, secreted from CASMCs, were primarily involved. mDCs adhesion to CASMCs was enhanced by CASMC pre-treatment with IFNγ and TNFα ICAM-1 and VCAM-1 were involved, since the expression of specific mRNAs for these molecules increased and adhesion was inhibited by neutralizing antibodies to the counter-receptors CD11c and CD18. Adhesion was also inhibited by CASMC pre-treatment with the HMG-CoA-reductase inhibitor atorvastatin and the PPARγ agonist rosiglitazone, which suggests a further mechanism for the anti-inflammatory action of these drugs. Adhesion of DCs to VSMCs was shown also in vivo in rat carotid 7 to 21 days after crush and incision injury. The findings indicate that DCs and VSMCs can interact with reciprocal stimulation, possibly leading to perpetuate inflammation and vascular wall remodelling, and that the interaction is enhanced by a cytokine-rich inflammatory environment and down-regulated by HMGCoA-reductase inhibitors and PPARγ agonists.Sara PaccosiClaudia MusilliRoberto CaporaleAnna Maria Grazia GelliDaniele GuastiAnn Maria ClementeMaria Gabriella TorciaAmelia FilippelliPaolo RomagnoliAstrid ParentiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99652 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara Paccosi Claudia Musilli Roberto Caporale Anna Maria Grazia Gelli Daniele Guasti Ann Maria Clemente Maria Gabriella Torcia Amelia Filippelli Paolo Romagnoli Astrid Parenti Stimulatory interactions between human coronary smooth muscle cells and dendritic cells.
description	Despite inflammatory and immune mechanisms participating to atherogenesis and dendritic cells (DCs) driving immune and non-immune tissue injury response, the interactions between DCs and vascular smooth muscle cells (VSMCs) possibly relevant to vascular pathology including atherogenesis are still unclear. To address this issue, immature DCs (iDCs) generated from CD14+ cells isolated from healthy donors were matured either with cytokines (mDCs), or co-cultured (ccDCs) with human coronary artery VSMCs (CASMCs) using transwell chambers. Co-culture induced DC immunophenotypical and functional maturation similar to cytokines, as demonstrated by flow cytometry and mixed lymphocyte reaction. In turn, factors from mDCs and ccDCs induced CASMC migration. MCP-1 and TNFα, secreted from DCs, and IL-6 and MCP-1, secreted from CASMCs, were primarily involved. mDCs adhesion to CASMCs was enhanced by CASMC pre-treatment with IFNγ and TNFα ICAM-1 and VCAM-1 were involved, since the expression of specific mRNAs for these molecules increased and adhesion was inhibited by neutralizing antibodies to the counter-receptors CD11c and CD18. Adhesion was also inhibited by CASMC pre-treatment with the HMG-CoA-reductase inhibitor atorvastatin and the PPARγ agonist rosiglitazone, which suggests a further mechanism for the anti-inflammatory action of these drugs. Adhesion of DCs to VSMCs was shown also in vivo in rat carotid 7 to 21 days after crush and incision injury. The findings indicate that DCs and VSMCs can interact with reciprocal stimulation, possibly leading to perpetuate inflammation and vascular wall remodelling, and that the interaction is enhanced by a cytokine-rich inflammatory environment and down-regulated by HMGCoA-reductase inhibitors and PPARγ agonists.
format	article
author	Sara Paccosi Claudia Musilli Roberto Caporale Anna Maria Grazia Gelli Daniele Guasti Ann Maria Clemente Maria Gabriella Torcia Amelia Filippelli Paolo Romagnoli Astrid Parenti
author_facet	Sara Paccosi Claudia Musilli Roberto Caporale Anna Maria Grazia Gelli Daniele Guasti Ann Maria Clemente Maria Gabriella Torcia Amelia Filippelli Paolo Romagnoli Astrid Parenti
author_sort	Sara Paccosi
title	Stimulatory interactions between human coronary smooth muscle cells and dendritic cells.
title_short	Stimulatory interactions between human coronary smooth muscle cells and dendritic cells.
title_full	Stimulatory interactions between human coronary smooth muscle cells and dendritic cells.
title_fullStr	Stimulatory interactions between human coronary smooth muscle cells and dendritic cells.
title_full_unstemmed	Stimulatory interactions between human coronary smooth muscle cells and dendritic cells.
title_sort	stimulatory interactions between human coronary smooth muscle cells and dendritic cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0cb6ca6d1bbb4e47939d013afd5a52b3
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Stimulatory interactions between human coronary smooth muscle cells and dendritic cells.

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