Brown Adipose Transplantation Improves Polycystic Ovary Syndrome-Involved Metabolome Remodeling

Polycystic ovary syndrome (PCOS) is a complex reproductive, endocrine, and metabolic disorder in reproductive-age women. In order to explore the active metabolites of brown adipose tissue (BAT) transplantation in improving the reproductive and metabolic phenotypes in a PCOS rat model, the metabolite...

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Autores principales:	Lihua Yao, Qin Wang, Runjie Zhang, Xingyun Wang, Yiwen Liu, Fangfang Di, Liwen Song, Siliang Xu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	brown adipose tissue metabolites LC-MS sphingosine PCOS Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/0cb77a19f25940129ee4670308f66306
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spelling	oai:doaj.org-article:0cb77a19f25940129ee4670308f663062021-12-01T13:26:36ZBrown Adipose Transplantation Improves Polycystic Ovary Syndrome-Involved Metabolome Remodeling1664-239210.3389/fendo.2021.747944https://doaj.org/article/0cb77a19f25940129ee4670308f663062021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.747944/fullhttps://doaj.org/toc/1664-2392Polycystic ovary syndrome (PCOS) is a complex reproductive, endocrine, and metabolic disorder in reproductive-age women. In order to explore the active metabolites of brown adipose tissue (BAT) transplantation in improving the reproductive and metabolic phenotypes in a PCOS rat model, the metabolites in the recipient’s BAT were explored using the liquid chromatography–mass spectrometry technique. In total, 9 upregulated and 13 downregulated metabolites were identified. They were roughly categorized into 12 distinct classes, mainly including glycerophosphoinositols, glycerophosphocholines, and sphingolipids. Ingenuity pathway analysis predicted that these differentially metabolites mainly target the PI3K/AKT, MAPK, and Wnt signaling pathways, which are closely associated with PCOS. Furthermore, one of these differential metabolites, sphingosine belonging to sphingolipids, was randomly selected for further experiments on a human granulosa-like tumor cell line (KGN). It significantly accelerated the apoptosis of KGN cells induced by dihydrotestosterone. Based on these findings, we speculated that metabolome changes are an important process for BAT transplantation in improving PCOS. It might be a novel therapeutic target for PCOS treatment.Lihua YaoLihua YaoQin WangQin WangRunjie ZhangRunjie ZhangXingyun WangXingyun WangYiwen LiuYiwen LiuFangfang DiFangfang DiLiwen SongLiwen SongSiliang XuFrontiers Media S.A.articlebrown adipose tissuemetabolitesLC-MSsphingosinePCOSDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	brown adipose tissue metabolites LC-MS sphingosine PCOS Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	brown adipose tissue metabolites LC-MS sphingosine PCOS Diseases of the endocrine glands. Clinical endocrinology RC648-665 Lihua Yao Lihua Yao Qin Wang Qin Wang Runjie Zhang Runjie Zhang Xingyun Wang Xingyun Wang Yiwen Liu Yiwen Liu Fangfang Di Fangfang Di Liwen Song Liwen Song Siliang Xu Brown Adipose Transplantation Improves Polycystic Ovary Syndrome-Involved Metabolome Remodeling
description	Polycystic ovary syndrome (PCOS) is a complex reproductive, endocrine, and metabolic disorder in reproductive-age women. In order to explore the active metabolites of brown adipose tissue (BAT) transplantation in improving the reproductive and metabolic phenotypes in a PCOS rat model, the metabolites in the recipient’s BAT were explored using the liquid chromatography–mass spectrometry technique. In total, 9 upregulated and 13 downregulated metabolites were identified. They were roughly categorized into 12 distinct classes, mainly including glycerophosphoinositols, glycerophosphocholines, and sphingolipids. Ingenuity pathway analysis predicted that these differentially metabolites mainly target the PI3K/AKT, MAPK, and Wnt signaling pathways, which are closely associated with PCOS. Furthermore, one of these differential metabolites, sphingosine belonging to sphingolipids, was randomly selected for further experiments on a human granulosa-like tumor cell line (KGN). It significantly accelerated the apoptosis of KGN cells induced by dihydrotestosterone. Based on these findings, we speculated that metabolome changes are an important process for BAT transplantation in improving PCOS. It might be a novel therapeutic target for PCOS treatment.
format	article
author	Lihua Yao Lihua Yao Qin Wang Qin Wang Runjie Zhang Runjie Zhang Xingyun Wang Xingyun Wang Yiwen Liu Yiwen Liu Fangfang Di Fangfang Di Liwen Song Liwen Song Siliang Xu
author_facet	Lihua Yao Lihua Yao Qin Wang Qin Wang Runjie Zhang Runjie Zhang Xingyun Wang Xingyun Wang Yiwen Liu Yiwen Liu Fangfang Di Fangfang Di Liwen Song Liwen Song Siliang Xu
author_sort	Lihua Yao
title	Brown Adipose Transplantation Improves Polycystic Ovary Syndrome-Involved Metabolome Remodeling
title_short	Brown Adipose Transplantation Improves Polycystic Ovary Syndrome-Involved Metabolome Remodeling
title_full	Brown Adipose Transplantation Improves Polycystic Ovary Syndrome-Involved Metabolome Remodeling
title_fullStr	Brown Adipose Transplantation Improves Polycystic Ovary Syndrome-Involved Metabolome Remodeling
title_full_unstemmed	Brown Adipose Transplantation Improves Polycystic Ovary Syndrome-Involved Metabolome Remodeling
title_sort	brown adipose transplantation improves polycystic ovary syndrome-involved metabolome remodeling
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0cb77a19f25940129ee4670308f66306
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Brown Adipose Transplantation Improves Polycystic Ovary Syndrome-Involved Metabolome Remodeling

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