Identification of Potential Key Genes Involved in the Carotid Atherosclerosis

Youshi Meng,1,2,* Chunli Zhang,1,2,* Lucong Liang,1,2,* Lei Wei,1,2 Hao Wang,1,2 Fengkun Zhou,1,2 Rongjie Li,1,2 Donghua Zou,1,2 Xiaohua Huang,3 Jie Liu4 1Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530022, People’s Republic of Ch...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Meng Y, Zhang C, Liang L, Wei L, Wang H, Zhou F, Li R, Zou D, Huang X, Liu J
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2021
Materias:
Acceso en línea:https://doaj.org/article/0cb87e01cd0d4d7395c37b26cfe6db3c
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:0cb87e01cd0d4d7395c37b26cfe6db3c
record_format dspace
spelling oai:doaj.org-article:0cb87e01cd0d4d7395c37b26cfe6db3c2021-12-02T17:37:19ZIdentification of Potential Key Genes Involved in the Carotid Atherosclerosis1178-1998https://doaj.org/article/0cb87e01cd0d4d7395c37b26cfe6db3c2021-06-01T00:00:00Zhttps://www.dovepress.com/identification-of-potential-key-genes-involved-in-the-carotid-atherosc-peer-reviewed-fulltext-article-CIAhttps://doaj.org/toc/1178-1998Youshi Meng,1,2,* Chunli Zhang,1,2,* Lucong Liang,1,2,* Lei Wei,1,2 Hao Wang,1,2 Fengkun Zhou,1,2 Rongjie Li,1,2 Donghua Zou,1,2 Xiaohua Huang,3 Jie Liu4 1Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530022, People’s Republic of China; 2Department of Neurology, The First People’s Hospital of Nanning, Nanning, Guangxi, 530022, People’s Republic of China; 3Department of Neurology, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, 533000, People’s Republic of China; 4Department of Cardiology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaohua HuangDepartment of Neurology, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, 533000, People’s Republic of ChinaEmail newxiaohua66@163.comJie LiuDepartment of Cardiology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530022, People’s Republic of ChinaTel +867712636189Email gxnnliujie@163.comPurpose: Carotid atherosclerosis is a kind of systemic atherosclerosis in the carotid arteries. However, the efficiency of treatment is insufficient. Therefore, it is urgent to find therapeutic targets and deepen the understanding of carotid atherosclerosis.Materials and Methods: In this study, we analyzed differentially expressed genes (DEGs) between atheroma plaque and macroscopically intact tissue (control samples). Furthermore, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) enrichment analysis based on the DEGs. Four methods were used to identify the hub genes in the protein–protein interaction networks of the DEGs. Furthermore, we also performed network module analysis to reveal carotid atherosclerosis-related gene modules and biological functions.Results: The enrichment results showed that the biological functions were related to inflammation, immunity, chemokine and cell adhesion molecule, such as PIK-Akt signaling pathway, Rap1 signaling pathway, MAPK signaling pathway, NOD-like receptor signaling pathway and B cell receptor signaling pathway. In addition, we screened the hub genes. A total of 16 up-regulated genes (C3AR1, CCR1, CCR2, CD33, CD53, CXCL10, CXCL8, CXCR4, CYBB, FCER1G, FPR2, ITGAL, ITGAM, ITGAX, ITGB2, and LILRB2) were identified as hub genes. A total of 5 gene modules were obtained. We found that biological functions obtained for each cluster were mostly related to immunity, chemokines and cell adhesion molecules.Conclusion: The present study identified key DEGs in atheroma plaque compared with control samples. The key genes involved in the development of carotid atherosclerosis may provide valuable therapeutic targets for carotid atherosclerosis.Keywords: identification of key genes, carotid atherosclerosis, cluster analysisMeng YZhang CLiang LWei LWang HZhou FLi RZou DHuang XLiu JDove Medical Pressarticleidentification of key genescarotid atherosclerosiscluster analysisGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 16, Pp 1071-1084 (2021)
institution DOAJ
collection DOAJ
language EN
topic identification of key genes
carotid atherosclerosis
cluster analysis
Geriatrics
RC952-954.6
spellingShingle identification of key genes
carotid atherosclerosis
cluster analysis
Geriatrics
RC952-954.6
Meng Y
Zhang C
Liang L
Wei L
Wang H
Zhou F
Li R
Zou D
Huang X
Liu J
Identification of Potential Key Genes Involved in the Carotid Atherosclerosis
description Youshi Meng,1,2,* Chunli Zhang,1,2,* Lucong Liang,1,2,* Lei Wei,1,2 Hao Wang,1,2 Fengkun Zhou,1,2 Rongjie Li,1,2 Donghua Zou,1,2 Xiaohua Huang,3 Jie Liu4 1Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530022, People’s Republic of China; 2Department of Neurology, The First People’s Hospital of Nanning, Nanning, Guangxi, 530022, People’s Republic of China; 3Department of Neurology, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, 533000, People’s Republic of China; 4Department of Cardiology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaohua HuangDepartment of Neurology, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, 533000, People’s Republic of ChinaEmail newxiaohua66@163.comJie LiuDepartment of Cardiology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530022, People’s Republic of ChinaTel +867712636189Email gxnnliujie@163.comPurpose: Carotid atherosclerosis is a kind of systemic atherosclerosis in the carotid arteries. However, the efficiency of treatment is insufficient. Therefore, it is urgent to find therapeutic targets and deepen the understanding of carotid atherosclerosis.Materials and Methods: In this study, we analyzed differentially expressed genes (DEGs) between atheroma plaque and macroscopically intact tissue (control samples). Furthermore, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) enrichment analysis based on the DEGs. Four methods were used to identify the hub genes in the protein–protein interaction networks of the DEGs. Furthermore, we also performed network module analysis to reveal carotid atherosclerosis-related gene modules and biological functions.Results: The enrichment results showed that the biological functions were related to inflammation, immunity, chemokine and cell adhesion molecule, such as PIK-Akt signaling pathway, Rap1 signaling pathway, MAPK signaling pathway, NOD-like receptor signaling pathway and B cell receptor signaling pathway. In addition, we screened the hub genes. A total of 16 up-regulated genes (C3AR1, CCR1, CCR2, CD33, CD53, CXCL10, CXCL8, CXCR4, CYBB, FCER1G, FPR2, ITGAL, ITGAM, ITGAX, ITGB2, and LILRB2) were identified as hub genes. A total of 5 gene modules were obtained. We found that biological functions obtained for each cluster were mostly related to immunity, chemokines and cell adhesion molecules.Conclusion: The present study identified key DEGs in atheroma plaque compared with control samples. The key genes involved in the development of carotid atherosclerosis may provide valuable therapeutic targets for carotid atherosclerosis.Keywords: identification of key genes, carotid atherosclerosis, cluster analysis
format article
author Meng Y
Zhang C
Liang L
Wei L
Wang H
Zhou F
Li R
Zou D
Huang X
Liu J
author_facet Meng Y
Zhang C
Liang L
Wei L
Wang H
Zhou F
Li R
Zou D
Huang X
Liu J
author_sort Meng Y
title Identification of Potential Key Genes Involved in the Carotid Atherosclerosis
title_short Identification of Potential Key Genes Involved in the Carotid Atherosclerosis
title_full Identification of Potential Key Genes Involved in the Carotid Atherosclerosis
title_fullStr Identification of Potential Key Genes Involved in the Carotid Atherosclerosis
title_full_unstemmed Identification of Potential Key Genes Involved in the Carotid Atherosclerosis
title_sort identification of potential key genes involved in the carotid atherosclerosis
publisher Dove Medical Press
publishDate 2021
url https://doaj.org/article/0cb87e01cd0d4d7395c37b26cfe6db3c
work_keys_str_mv AT mengy identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
AT zhangc identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
AT liangl identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
AT weil identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
AT wangh identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
AT zhouf identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
AT lir identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
AT zoud identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
AT huangx identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
AT liuj identificationofpotentialkeygenesinvolvedinthecarotidatherosclerosis
_version_ 1718379865404604416