pH-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma LoVo cells.

<h4>Background</h4>The triblock copolymers PEG-P(Asp-DIP)-P(Lys-Ca) (PEALCa) of polyethylene glycol (PEG), poly(N-(N',N'-diisopropylaminoethyl) aspartamide) (P(Asp-DIP)), and poly (lysine-cholic acid) (P(Lys-Ca)) were synthesized as a pH-sensitive drug delivery system. In neutr...

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Autores principales:	Shi-Ting Feng, Jingguo Li, Yanji Luo, Tinghui Yin, Huasong Cai, Yong Wang, Zhi Dong, Xintao Shuai, Zi-Ping Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cbb506dfb4141a79dbe2a56ad32e109
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spelling	oai:doaj.org-article:0cbb506dfb4141a79dbe2a56ad32e1092021-11-11T08:21:36ZpH-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma LoVo cells.1932-620310.1371/journal.pone.0100732https://doaj.org/article/0cbb506dfb4141a79dbe2a56ad32e1092014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24964012/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The triblock copolymers PEG-P(Asp-DIP)-P(Lys-Ca) (PEALCa) of polyethylene glycol (PEG), poly(N-(N',N'-diisopropylaminoethyl) aspartamide) (P(Asp-DIP)), and poly (lysine-cholic acid) (P(Lys-Ca)) were synthesized as a pH-sensitive drug delivery system. In neutral aqueous environment such as physiological environment, PEALCa can self-assemble into stable vesicles with a size around 50-60 nm, avoid uptake by the reticuloendothelial system (RES), and encase the drug in the core. However, the PEALCa micelles disassemble and release drug rapidly in acidic environment that resembles lysosomal compartments.<h4>Methodology/principal findings</h4>The anticancer drug Paclitaxel (PTX) and hydrophilic superparamagnetic iron oxide (SPIO) were encapsulated inside the core of the PEALCa micelles and used for potential cancer therapy. Drug release study revealed that PTX in the micelles was released faster at pH 5.0 than at pH 7.4. Cell culture studies showed that the PTX-SPIO-PEALCa micelle was effectively internalized by human colon carcinoma cell line (LoVo cells), and PTX could be embedded inside lysosomal compartments. Moreover, the human colorectal carcinoma (CRC) LoVo cells delivery effect was verified in vivo by magnetic resonance imaging (MRI) and histology analysis. Consequently effective suppression of CRC LoVo cell growth was evaluated.<h4>Conclusions/significance</h4>These results indicated that the PTX-SPION-loaded pH-sensitive micelles were a promising MRI-visible drug release system for colorectal cancer therapy.Shi-Ting FengJingguo LiYanji LuoTinghui YinHuasong CaiYong WangZhi DongXintao ShuaiZi-Ping LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100732 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shi-Ting Feng Jingguo Li Yanji Luo Tinghui Yin Huasong Cai Yong Wang Zhi Dong Xintao Shuai Zi-Ping Li pH-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma LoVo cells.
description	<h4>Background</h4>The triblock copolymers PEG-P(Asp-DIP)-P(Lys-Ca) (PEALCa) of polyethylene glycol (PEG), poly(N-(N',N'-diisopropylaminoethyl) aspartamide) (P(Asp-DIP)), and poly (lysine-cholic acid) (P(Lys-Ca)) were synthesized as a pH-sensitive drug delivery system. In neutral aqueous environment such as physiological environment, PEALCa can self-assemble into stable vesicles with a size around 50-60 nm, avoid uptake by the reticuloendothelial system (RES), and encase the drug in the core. However, the PEALCa micelles disassemble and release drug rapidly in acidic environment that resembles lysosomal compartments.<h4>Methodology/principal findings</h4>The anticancer drug Paclitaxel (PTX) and hydrophilic superparamagnetic iron oxide (SPIO) were encapsulated inside the core of the PEALCa micelles and used for potential cancer therapy. Drug release study revealed that PTX in the micelles was released faster at pH 5.0 than at pH 7.4. Cell culture studies showed that the PTX-SPIO-PEALCa micelle was effectively internalized by human colon carcinoma cell line (LoVo cells), and PTX could be embedded inside lysosomal compartments. Moreover, the human colorectal carcinoma (CRC) LoVo cells delivery effect was verified in vivo by magnetic resonance imaging (MRI) and histology analysis. Consequently effective suppression of CRC LoVo cell growth was evaluated.<h4>Conclusions/significance</h4>These results indicated that the PTX-SPION-loaded pH-sensitive micelles were a promising MRI-visible drug release system for colorectal cancer therapy.
format	article
author	Shi-Ting Feng Jingguo Li Yanji Luo Tinghui Yin Huasong Cai Yong Wang Zhi Dong Xintao Shuai Zi-Ping Li
author_facet	Shi-Ting Feng Jingguo Li Yanji Luo Tinghui Yin Huasong Cai Yong Wang Zhi Dong Xintao Shuai Zi-Ping Li
author_sort	Shi-Ting Feng
title	pH-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma LoVo cells.
title_short	pH-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma LoVo cells.
title_full	pH-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma LoVo cells.
title_fullStr	pH-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma LoVo cells.
title_full_unstemmed	pH-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma LoVo cells.
title_sort	ph-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma lovo cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0cbb506dfb4141a79dbe2a56ad32e109
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pH-sensitive nanomicelles for controlled and efficient drug delivery to human colorectal carcinoma LoVo cells.

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