Class I ADP-ribosylation factors are involved in enterovirus 71 replication.

Enterovirus 71 is one of the major causative agents of hand, foot, and mouth disease in infants and children. Replication of enterovirus 71 depends on host cellular factors. The viral replication complex is formed in novel, cytoplasmic, vesicular compartments. It has not been elucidated which cellul...

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Autores principales:	Jianmin Wang, Jiang Du, Qi Jin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cbbf9f873bc4c50ac1200297cfa5412
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spelling	oai:doaj.org-article:0cbbf9f873bc4c50ac1200297cfa54122021-11-18T08:16:31ZClass I ADP-ribosylation factors are involved in enterovirus 71 replication.1932-620310.1371/journal.pone.0099768https://doaj.org/article/0cbbf9f873bc4c50ac1200297cfa54122014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24911624/?tool=EBIhttps://doaj.org/toc/1932-6203Enterovirus 71 is one of the major causative agents of hand, foot, and mouth disease in infants and children. Replication of enterovirus 71 depends on host cellular factors. The viral replication complex is formed in novel, cytoplasmic, vesicular compartments. It has not been elucidated which cellular pathways are hijacked by the virus to create these vesicles. Here, we investigated whether proteins associated with the cellular secretory pathway were involved in enterovirus 71 replication. We used a loss-of-function assay, based on small interfering RNA. We showed that enterovirus 71 RNA replication was dependent on the activity of Class I ADP-ribosylation factors. Simultaneous depletion of ADP-ribosylation factors 1 and 3, but not three others, inhibited viral replication in cells. We also demonstrated with various techniques that the brefeldin-A-sensitive guanidine nucleotide exchange factor, GBF1, was critically important for enterovirus 71 replication. Our results suggested that enterovirus 71 replication depended on GBF1-mediated activation of Class I ADP-ribosylation factors. These results revealed a connection between enterovirus 71 replication and the cellular secretory pathway; this pathway may represent a novel target for antiviral therapies.Jianmin WangJiang DuQi JinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99768 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jianmin Wang Jiang Du Qi Jin Class I ADP-ribosylation factors are involved in enterovirus 71 replication.
description	Enterovirus 71 is one of the major causative agents of hand, foot, and mouth disease in infants and children. Replication of enterovirus 71 depends on host cellular factors. The viral replication complex is formed in novel, cytoplasmic, vesicular compartments. It has not been elucidated which cellular pathways are hijacked by the virus to create these vesicles. Here, we investigated whether proteins associated with the cellular secretory pathway were involved in enterovirus 71 replication. We used a loss-of-function assay, based on small interfering RNA. We showed that enterovirus 71 RNA replication was dependent on the activity of Class I ADP-ribosylation factors. Simultaneous depletion of ADP-ribosylation factors 1 and 3, but not three others, inhibited viral replication in cells. We also demonstrated with various techniques that the brefeldin-A-sensitive guanidine nucleotide exchange factor, GBF1, was critically important for enterovirus 71 replication. Our results suggested that enterovirus 71 replication depended on GBF1-mediated activation of Class I ADP-ribosylation factors. These results revealed a connection between enterovirus 71 replication and the cellular secretory pathway; this pathway may represent a novel target for antiviral therapies.
format	article
author	Jianmin Wang Jiang Du Qi Jin
author_facet	Jianmin Wang Jiang Du Qi Jin
author_sort	Jianmin Wang
title	Class I ADP-ribosylation factors are involved in enterovirus 71 replication.
title_short	Class I ADP-ribosylation factors are involved in enterovirus 71 replication.
title_full	Class I ADP-ribosylation factors are involved in enterovirus 71 replication.
title_fullStr	Class I ADP-ribosylation factors are involved in enterovirus 71 replication.
title_full_unstemmed	Class I ADP-ribosylation factors are involved in enterovirus 71 replication.
title_sort	class i adp-ribosylation factors are involved in enterovirus 71 replication.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0cbbf9f873bc4c50ac1200297cfa5412
work_keys_str_mv	AT jianminwang classiadpribosylationfactorsareinvolvedinenterovirus71replication AT jiangdu classiadpribosylationfactorsareinvolvedinenterovirus71replication AT qijin classiadpribosylationfactorsareinvolvedinenterovirus71replication
_version_	1718421966476541952

Class I ADP-ribosylation factors are involved in enterovirus 71 replication.

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