A surface plasmon resonance based approach for measuring response to pneumococcal vaccine
Abstract Incidence of pneumococcal disease has increased worldwide in recent years. Response to pneumococcal vaccine is usually measured using the multiserotype enzyme-linked immunosorbent assay (ELISA) pneumococcal test. However, this approach presents several limitations. Therefore, the introducti...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/0cc2b5b8e2804eda85ce01928ae32afd |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:0cc2b5b8e2804eda85ce01928ae32afd |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:0cc2b5b8e2804eda85ce01928ae32afd2021-12-02T13:24:14ZA surface plasmon resonance based approach for measuring response to pneumococcal vaccine10.1038/s41598-021-85958-02045-2322https://doaj.org/article/0cc2b5b8e2804eda85ce01928ae32afd2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85958-0https://doaj.org/toc/2045-2322Abstract Incidence of pneumococcal disease has increased worldwide in recent years. Response to pneumococcal vaccine is usually measured using the multiserotype enzyme-linked immunosorbent assay (ELISA) pneumococcal test. However, this approach presents several limitations. Therefore, the introduction of new and more robust analytical approaches able to provide information on the efficacy of the pneumococcal vaccine would be very beneficial for the clinical management of patients. Surface plasmon resonance (SPR) has been shown to offer a valuable understanding of vaccines’ properties over the last years. The aim of this study is to evaluate the reliability of SPR for the anti-pneumococcal capsular polysaccharides (anti-PnPs) IgGs quantification in vaccinated. Fast protein liquid chromatography (FPLC) was used for the isolation of total IgGs from serum samples of vaccinated patients. Binding-SPR assays were performed to study the interaction between anti-PnPs IgGs and PCV13. A robust correlation was found between serum levels of anti-PnPs IgGs, measured by ELISA, and the SPR signal. Moreover, it was possible to correctly classify patients into “non-responder”, “responder” and “high-responder” groups according to their specific SPR PCV13 response profiles. SPR technology provides a valuable tool for reliably characterize the interaction between anti-PnPs IgGs and PCV13 in a very short experimental time.Marta Garrido-JareñoLeonor Puchades-CarrascoLeticia Orti-PérezJosé Miguel Sahuquillo-ArceMaría del Carmen Meyer-GarcíaJoan Mollar-MaseresCarmina Lloret-SosAna Gil-BrusolaJosé Luis López-HontangasJosé Manuel Beltrán-GarridoJavier Pemán-GarcíaAntonio Pineda-LucenaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Marta Garrido-Jareño Leonor Puchades-Carrasco Leticia Orti-Pérez José Miguel Sahuquillo-Arce María del Carmen Meyer-García Joan Mollar-Maseres Carmina Lloret-Sos Ana Gil-Brusola José Luis López-Hontangas José Manuel Beltrán-Garrido Javier Pemán-García Antonio Pineda-Lucena A surface plasmon resonance based approach for measuring response to pneumococcal vaccine |
| description |
Abstract Incidence of pneumococcal disease has increased worldwide in recent years. Response to pneumococcal vaccine is usually measured using the multiserotype enzyme-linked immunosorbent assay (ELISA) pneumococcal test. However, this approach presents several limitations. Therefore, the introduction of new and more robust analytical approaches able to provide information on the efficacy of the pneumococcal vaccine would be very beneficial for the clinical management of patients. Surface plasmon resonance (SPR) has been shown to offer a valuable understanding of vaccines’ properties over the last years. The aim of this study is to evaluate the reliability of SPR for the anti-pneumococcal capsular polysaccharides (anti-PnPs) IgGs quantification in vaccinated. Fast protein liquid chromatography (FPLC) was used for the isolation of total IgGs from serum samples of vaccinated patients. Binding-SPR assays were performed to study the interaction between anti-PnPs IgGs and PCV13. A robust correlation was found between serum levels of anti-PnPs IgGs, measured by ELISA, and the SPR signal. Moreover, it was possible to correctly classify patients into “non-responder”, “responder” and “high-responder” groups according to their specific SPR PCV13 response profiles. SPR technology provides a valuable tool for reliably characterize the interaction between anti-PnPs IgGs and PCV13 in a very short experimental time. |
| format |
article |
| author |
Marta Garrido-Jareño Leonor Puchades-Carrasco Leticia Orti-Pérez José Miguel Sahuquillo-Arce María del Carmen Meyer-García Joan Mollar-Maseres Carmina Lloret-Sos Ana Gil-Brusola José Luis López-Hontangas José Manuel Beltrán-Garrido Javier Pemán-García Antonio Pineda-Lucena |
| author_facet |
Marta Garrido-Jareño Leonor Puchades-Carrasco Leticia Orti-Pérez José Miguel Sahuquillo-Arce María del Carmen Meyer-García Joan Mollar-Maseres Carmina Lloret-Sos Ana Gil-Brusola José Luis López-Hontangas José Manuel Beltrán-Garrido Javier Pemán-García Antonio Pineda-Lucena |
| author_sort |
Marta Garrido-Jareño |
| title |
A surface plasmon resonance based approach for measuring response to pneumococcal vaccine |
| title_short |
A surface plasmon resonance based approach for measuring response to pneumococcal vaccine |
| title_full |
A surface plasmon resonance based approach for measuring response to pneumococcal vaccine |
| title_fullStr |
A surface plasmon resonance based approach for measuring response to pneumococcal vaccine |
| title_full_unstemmed |
A surface plasmon resonance based approach for measuring response to pneumococcal vaccine |
| title_sort |
surface plasmon resonance based approach for measuring response to pneumococcal vaccine |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/0cc2b5b8e2804eda85ce01928ae32afd |
| work_keys_str_mv |
AT martagarridojareno asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT leonorpuchadescarrasco asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT leticiaortiperez asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT josemiguelsahuquilloarce asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT mariadelcarmenmeyergarcia asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT joanmollarmaseres asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT carminalloretsos asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT anagilbrusola asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT joseluislopezhontangas asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT josemanuelbeltrangarrido asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT javierpemangarcia asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT antoniopinedalucena asurfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT martagarridojareno surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT leonorpuchadescarrasco surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT leticiaortiperez surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT josemiguelsahuquilloarce surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT mariadelcarmenmeyergarcia surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT joanmollarmaseres surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT carminalloretsos surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT anagilbrusola surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT joseluislopezhontangas surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT josemanuelbeltrangarrido surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT javierpemangarcia surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine AT antoniopinedalucena surfaceplasmonresonancebasedapproachformeasuringresponsetopneumococcalvaccine |
| _version_ |
1718393074236784640 |