Genetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population

Abstract C-type lectin receptors (CLRs) have been demonstrated to be involved in several autoimmune diseases. The role of CLRs in Behcet’s disease (BD) is unknown and thus was the purpose of this study. A two-stage association study was carried out and a total of 766 BD patients and 1674 healthy con...

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Autores principales:	Yi Yang, Handan Tan, Bolin Deng, Hongsong Yu, Guannan Su, Jiayue Hu, Qingfeng Cao, Gangxiang Yuan, Aize Kijlstra, Peizeng Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cc81067e9c44f01954865d97e592c94
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spelling	oai:doaj.org-article:0cc81067e9c44f01954865d97e592c942021-12-02T12:32:33ZGenetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population10.1038/s41598-017-05877-x2045-2322https://doaj.org/article/0cc81067e9c44f01954865d97e592c942017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05877-xhttps://doaj.org/toc/2045-2322Abstract C-type lectin receptors (CLRs) have been demonstrated to be involved in several autoimmune diseases. The role of CLRs in Behcet’s disease (BD) is unknown and thus was the purpose of this study. A two-stage association study was carried out and a total of 766 BD patients and 1674 healthy controls were recruited. Genotyping of 14 SNPs of 13 genes in CLRs was carried out by iPLEX Gold genotyping or polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The expression of mannose binding lectin 2 (MBL2) and killer cell lectin like receptor C4 (KLRC4) was measured by Real-time PCR. Significantly increased frequencies of the A allele as well as AA genotype of rs1800450 in MBL2 (Pc = 2.50 × 10−6, OR = 1.494; Pc = 2.24 × 10−6,OR = 2.899; respectively) and TT genotype of rs2617170 in KLRC4 (Pc = 2.53 × 10−6, OR = 1.695) and decreased frequencies of GG genotype of rs1800450 (Pc = 1.56 × 10−3, OR = 0.689) and C allele as well as CC genotype of rs2617170 (Pc = 2.05 × 10−9,OR = 0.664; Pc = 1.20 × 10−5, OR = 0.585; respectively) were observed in BD. Two variants, p.Gly54Asp (rs1800450) and p.Asn104Ser (rs2617170) affect MBL2 and KLRC4 protein stability and expression. Our study demonstrates that the MBL2/rs1800450 and KLRC4/rs2617170 are susceptibility factors for BD in a Chinese Han population.Yi YangHandan TanBolin DengHongsong YuGuannan SuJiayue HuQingfeng CaoGangxiang YuanAize KijlstraPeizeng YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Yang Handan Tan Bolin Deng Hongsong Yu Guannan Su Jiayue Hu Qingfeng Cao Gangxiang Yuan Aize Kijlstra Peizeng Yang Genetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population
description	Abstract C-type lectin receptors (CLRs) have been demonstrated to be involved in several autoimmune diseases. The role of CLRs in Behcet’s disease (BD) is unknown and thus was the purpose of this study. A two-stage association study was carried out and a total of 766 BD patients and 1674 healthy controls were recruited. Genotyping of 14 SNPs of 13 genes in CLRs was carried out by iPLEX Gold genotyping or polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The expression of mannose binding lectin 2 (MBL2) and killer cell lectin like receptor C4 (KLRC4) was measured by Real-time PCR. Significantly increased frequencies of the A allele as well as AA genotype of rs1800450 in MBL2 (Pc = 2.50 × 10−6, OR = 1.494; Pc = 2.24 × 10−6,OR = 2.899; respectively) and TT genotype of rs2617170 in KLRC4 (Pc = 2.53 × 10−6, OR = 1.695) and decreased frequencies of GG genotype of rs1800450 (Pc = 1.56 × 10−3, OR = 0.689) and C allele as well as CC genotype of rs2617170 (Pc = 2.05 × 10−9,OR = 0.664; Pc = 1.20 × 10−5, OR = 0.585; respectively) were observed in BD. Two variants, p.Gly54Asp (rs1800450) and p.Asn104Ser (rs2617170) affect MBL2 and KLRC4 protein stability and expression. Our study demonstrates that the MBL2/rs1800450 and KLRC4/rs2617170 are susceptibility factors for BD in a Chinese Han population.
format	article
author	Yi Yang Handan Tan Bolin Deng Hongsong Yu Guannan Su Jiayue Hu Qingfeng Cao Gangxiang Yuan Aize Kijlstra Peizeng Yang
author_facet	Yi Yang Handan Tan Bolin Deng Hongsong Yu Guannan Su Jiayue Hu Qingfeng Cao Gangxiang Yuan Aize Kijlstra Peizeng Yang
author_sort	Yi Yang
title	Genetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population
title_short	Genetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population
title_full	Genetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population
title_fullStr	Genetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population
title_full_unstemmed	Genetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population
title_sort	genetic polymorphisms of c-type lectin receptors in behcet’s disease in a chinese han population
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0cc81067e9c44f01954865d97e592c94
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Genetic polymorphisms of C-type lectin receptors in Behcet’s disease in a Chinese Han population

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