Comparison of Gene Expression Between Resistant and Susceptible Families Against VPAHPND and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei

Acute hepatopancreatic necrosis disease (AHPND) has caused a heavy loss to shrimp aquaculture since its outbreak. Vibrio parahaemolyticus (VPAHPND) is regarded as one of the main pathogens that caused AHPND in the Pacific white shrimp Litopenaeus vannamei. In order to learn more about the mechanism...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qian Zhang, Yang Yu, Zheng Luo, Jianhai Xiang, Fuhua Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	gene expression disease resistance molecular marker Vibrio parahaemolyticus Litopenaeus vannamei Genetics QH426-470
Acceso en línea:	https://doaj.org/article/0ccfac51a9a0406894fdc595f5bcde0c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0ccfac51a9a0406894fdc595f5bcde0c
record_format	dspace
spelling	oai:doaj.org-article:0ccfac51a9a0406894fdc595f5bcde0c2021-12-01T07:35:48ZComparison of Gene Expression Between Resistant and Susceptible Families Against VPAHPND and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei1664-802110.3389/fgene.2021.772442https://doaj.org/article/0ccfac51a9a0406894fdc595f5bcde0c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.772442/fullhttps://doaj.org/toc/1664-8021Acute hepatopancreatic necrosis disease (AHPND) has caused a heavy loss to shrimp aquaculture since its outbreak. Vibrio parahaemolyticus (VPAHPND) is regarded as one of the main pathogens that caused AHPND in the Pacific white shrimp Litopenaeus vannamei. In order to learn more about the mechanism of resistance to AHPND, the resistant and susceptible shrimp families were obtained through genetic breeding, and comparative transcriptome approach was used to analyze the gene expression patterns between resistant and susceptible families. A total of 95 families were subjected to VPAHPND challenge test, and significant variations in the resistance of these families were observed. Three pairs of resistant and susceptible families were selected for transcriptome sequencing. A total of 489 differentially expressed genes (DEGs) that presented in at least two pairwise comparisons were screened, including 196 DEGs highly expressed in the susceptible families and 293 DEGs in the resistant families. Among these DEGs, 16 genes demonstrated significant difference in all three pairwise comparisons. Gene set enrichment analysis (GSEA) of all 27,331 expressed genes indicated that some energy metabolism processes were enriched in the resistant families, while signal transduction and immune system were enriched in the susceptible families. A total of 32 DEGs were further confirmed in the offspring of the detected families, among which 19 genes were successfully verified. The identified genes in this study will be useful for clarifying the genetic mechanism of shrimp resistance against Vibrio and will further provide molecular markers for evaluating the disease resistance of shrimp in the breeding program.Qian ZhangQian ZhangYang YuYang YuZheng LuoZheng LuoJianhai XiangJianhai XiangFuhua LiFuhua LiFuhua LiFuhua LiFrontiers Media S.A.articlegene expressiondisease resistancemolecular markerVibrio parahaemolyticusLitopenaeus vannameiGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gene expression disease resistance molecular marker Vibrio parahaemolyticus Litopenaeus vannamei Genetics QH426-470
spellingShingle	gene expression disease resistance molecular marker Vibrio parahaemolyticus Litopenaeus vannamei Genetics QH426-470 Qian Zhang Qian Zhang Yang Yu Yang Yu Zheng Luo Zheng Luo Jianhai Xiang Jianhai Xiang Fuhua Li Fuhua Li Fuhua Li Fuhua Li Comparison of Gene Expression Between Resistant and Susceptible Families Against VPAHPND and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei
description	Acute hepatopancreatic necrosis disease (AHPND) has caused a heavy loss to shrimp aquaculture since its outbreak. Vibrio parahaemolyticus (VPAHPND) is regarded as one of the main pathogens that caused AHPND in the Pacific white shrimp Litopenaeus vannamei. In order to learn more about the mechanism of resistance to AHPND, the resistant and susceptible shrimp families were obtained through genetic breeding, and comparative transcriptome approach was used to analyze the gene expression patterns between resistant and susceptible families. A total of 95 families were subjected to VPAHPND challenge test, and significant variations in the resistance of these families were observed. Three pairs of resistant and susceptible families were selected for transcriptome sequencing. A total of 489 differentially expressed genes (DEGs) that presented in at least two pairwise comparisons were screened, including 196 DEGs highly expressed in the susceptible families and 293 DEGs in the resistant families. Among these DEGs, 16 genes demonstrated significant difference in all three pairwise comparisons. Gene set enrichment analysis (GSEA) of all 27,331 expressed genes indicated that some energy metabolism processes were enriched in the resistant families, while signal transduction and immune system were enriched in the susceptible families. A total of 32 DEGs were further confirmed in the offspring of the detected families, among which 19 genes were successfully verified. The identified genes in this study will be useful for clarifying the genetic mechanism of shrimp resistance against Vibrio and will further provide molecular markers for evaluating the disease resistance of shrimp in the breeding program.
format	article
author	Qian Zhang Qian Zhang Yang Yu Yang Yu Zheng Luo Zheng Luo Jianhai Xiang Jianhai Xiang Fuhua Li Fuhua Li Fuhua Li Fuhua Li
author_facet	Qian Zhang Qian Zhang Yang Yu Yang Yu Zheng Luo Zheng Luo Jianhai Xiang Jianhai Xiang Fuhua Li Fuhua Li Fuhua Li Fuhua Li
author_sort	Qian Zhang
title	Comparison of Gene Expression Between Resistant and Susceptible Families Against VPAHPND and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei
title_short	Comparison of Gene Expression Between Resistant and Susceptible Families Against VPAHPND and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei
title_full	Comparison of Gene Expression Between Resistant and Susceptible Families Against VPAHPND and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei
title_fullStr	Comparison of Gene Expression Between Resistant and Susceptible Families Against VPAHPND and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei
title_full_unstemmed	Comparison of Gene Expression Between Resistant and Susceptible Families Against VPAHPND and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei
title_sort	comparison of gene expression between resistant and susceptible families against vpahpnd and identification of biomarkers used for resistance evaluation in litopenaeus vannamei
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0ccfac51a9a0406894fdc595f5bcde0c
work_keys_str_mv	AT qianzhang comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT qianzhang comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT yangyu comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT yangyu comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT zhengluo comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT zhengluo comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT jianhaixiang comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT jianhaixiang comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT fuhuali comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT fuhuali comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT fuhuali comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei AT fuhuali comparisonofgeneexpressionbetweenresistantandsusceptiblefamiliesagainstvpahpndandidentificationofbiomarkersusedforresistanceevaluationinlitopenaeusvannamei
_version_	1718405396729692160

Comparison of Gene Expression Between Resistant and Susceptible Families Against VPAHPND and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei

Ejemplares similares