Corneal stromal roughness after VisuMax and Intralase femtosecond laser photodisruption: An atomic force microscopy study.

<h4>Purpose</h4>To compare the induced corneal stromal bed roughness measured with atomic force microscopy (AFM) after LASIK flap creation with the IntraLase 60 kHz and the VisuMax femtosecond laser platforms.<h4>Methods</h4>Three freshly enucleated porcine eyes were operated...

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Autores principales:	Juan Gros-Otero, Samira Ketabi, Rafael Cañones-Zafra, Montserrat Garcia-Gonzalez, Cesar Villa-Collar, Santiago Casado, Miguel A Teus
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cd06a9bba6e4387a607e98e080c1a1c
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spelling	oai:doaj.org-article:0cd06a9bba6e4387a607e98e080c1a1c2021-12-02T20:11:13ZCorneal stromal roughness after VisuMax and Intralase femtosecond laser photodisruption: An atomic force microscopy study.1932-620310.1371/journal.pone.0252449https://doaj.org/article/0cd06a9bba6e4387a607e98e080c1a1c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252449https://doaj.org/toc/1932-6203<h4>Purpose</h4>To compare the induced corneal stromal bed roughness measured with atomic force microscopy (AFM) after LASIK flap creation with the IntraLase 60 kHz and the VisuMax femtosecond laser platforms.<h4>Methods</h4>Three freshly enucleated porcine eyes were operated with each femtosecond laser in this experimental study. Standard LASIK treatment parameters were used for the experiment. After LASIK flap creation, the corneal stromal roughness was assessed using a JPK NanoWizard II® AFM in contact mode immersed in liquid. Olympus OMCL-RC800PSA commercial silicon nitride cantilever tips were used. Surface measurements were made in 10 regions of the central cornea of each sample measuring 20 x 20 microns, at 512 x 512 point resolution. Roughness was measured using the root-mean-square (RMS) value within the given regions.<h4>Results</h4>Measurements from 30 regions of the 3 eyes (10 measurements per eye) in the Intralase (FS1) group, and 30 regions of the 3 eyes (10 measurements per eye) in the VisuMax (FS2) group were analyzed. There was a statistically significant difference in mean ± standard deviation RMS values between the FS1 and the FS2 groups (360 ± 120 versus 230 ± 100 nm respectively; P< 0.00001).<h4>Conclusion</h4>This AFM study indicates that the surface of the stromal bed after LASIK flap creation is smoother in the FS2 group than the FS1 group.Juan Gros-OteroSamira KetabiRafael Cañones-ZafraMontserrat Garcia-GonzalezCesar Villa-CollarSantiago CasadoMiguel A TeusPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0252449 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan Gros-Otero Samira Ketabi Rafael Cañones-Zafra Montserrat Garcia-Gonzalez Cesar Villa-Collar Santiago Casado Miguel A Teus Corneal stromal roughness after VisuMax and Intralase femtosecond laser photodisruption: An atomic force microscopy study.
description	<h4>Purpose</h4>To compare the induced corneal stromal bed roughness measured with atomic force microscopy (AFM) after LASIK flap creation with the IntraLase 60 kHz and the VisuMax femtosecond laser platforms.<h4>Methods</h4>Three freshly enucleated porcine eyes were operated with each femtosecond laser in this experimental study. Standard LASIK treatment parameters were used for the experiment. After LASIK flap creation, the corneal stromal roughness was assessed using a JPK NanoWizard II® AFM in contact mode immersed in liquid. Olympus OMCL-RC800PSA commercial silicon nitride cantilever tips were used. Surface measurements were made in 10 regions of the central cornea of each sample measuring 20 x 20 microns, at 512 x 512 point resolution. Roughness was measured using the root-mean-square (RMS) value within the given regions.<h4>Results</h4>Measurements from 30 regions of the 3 eyes (10 measurements per eye) in the Intralase (FS1) group, and 30 regions of the 3 eyes (10 measurements per eye) in the VisuMax (FS2) group were analyzed. There was a statistically significant difference in mean ± standard deviation RMS values between the FS1 and the FS2 groups (360 ± 120 versus 230 ± 100 nm respectively; P< 0.00001).<h4>Conclusion</h4>This AFM study indicates that the surface of the stromal bed after LASIK flap creation is smoother in the FS2 group than the FS1 group.
format	article
author	Juan Gros-Otero Samira Ketabi Rafael Cañones-Zafra Montserrat Garcia-Gonzalez Cesar Villa-Collar Santiago Casado Miguel A Teus
author_facet	Juan Gros-Otero Samira Ketabi Rafael Cañones-Zafra Montserrat Garcia-Gonzalez Cesar Villa-Collar Santiago Casado Miguel A Teus
author_sort	Juan Gros-Otero
title	Corneal stromal roughness after VisuMax and Intralase femtosecond laser photodisruption: An atomic force microscopy study.
title_short	Corneal stromal roughness after VisuMax and Intralase femtosecond laser photodisruption: An atomic force microscopy study.
title_full	Corneal stromal roughness after VisuMax and Intralase femtosecond laser photodisruption: An atomic force microscopy study.
title_fullStr	Corneal stromal roughness after VisuMax and Intralase femtosecond laser photodisruption: An atomic force microscopy study.
title_full_unstemmed	Corneal stromal roughness after VisuMax and Intralase femtosecond laser photodisruption: An atomic force microscopy study.
title_sort	corneal stromal roughness after visumax and intralase femtosecond laser photodisruption: an atomic force microscopy study.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0cd06a9bba6e4387a607e98e080c1a1c
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Corneal stromal roughness after VisuMax and Intralase femtosecond laser photodisruption: An atomic force microscopy study.

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