Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains

Abstract Ethanolamine plasmalogens (EPls), unique alkenylacyl-glycerophospholipids, are the only known ligands of G-protein-coupled receptor 61—a novel receptor co-localised with gonadotropin-releasing hormone receptors on anterior pituitary gonadotrophs. Brain EPl decreases with age. Commercial EPl...

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Autores principales:	Hiroya Kadokawa, Miyako Kotaniguchi, Onalenna Kereilwe, Shinichi Kitamura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cd4c556ea644850bb345b9d0325fc9a
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spelling	oai:doaj.org-article:0cd4c556ea644850bb345b9d0325fc9a2021-12-02T15:54:06ZReduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains10.1038/s41598-021-84306-62045-2322https://doaj.org/article/0cd4c556ea644850bb345b9d0325fc9a2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84306-6https://doaj.org/toc/2045-2322Abstract Ethanolamine plasmalogens (EPls), unique alkenylacyl-glycerophospholipids, are the only known ligands of G-protein-coupled receptor 61—a novel receptor co-localised with gonadotropin-releasing hormone receptors on anterior pituitary gonadotrophs. Brain EPl decreases with age. Commercial EPl—extracted from the cattle brain (unidentified age)—can independently stimulate FSH secretion from gonadotrophs. We hypothesised that there exists an age-related difference in the quality, quantity, and ability of bovine brain EPls to stimulate bovine gonadotrophs. We compared the brains of young (about 26 month old heifers) and old (about 90 month old cows) Japanese Black bovines, including EPls obtained from both groups. Additionally, mRNA expressions of the EPl biosynthesis enzymes, glyceronephosphate O-acyltransferase, alkylglycerone phosphate synthase, and fatty acyl-CoA reductase 1 (FAR1) were evaluated in young and old hypothalami. The old-brain EPl did not stimulate FSH secretion from gonadotrophs, unlike the young-brain EPl. Molecular species of EPl were compared using two-dimensional liquid chromatography-mass spectrometry. We identified 20 EPl molecular species of which three and three exhibited lower (P < 0.05) and higher (P < 0.05) ratios, respectively, in old compared to young brains. In addition, quantitative reverse transcription-polymerase chain reaction detected higher FAR1 levels in the POA, but not in the ARC&ME tissues, of old cows than that of fertile young heifers. Therefore, old-brain EPl may be associated with age-related infertility.Hiroya KadokawaMiyako KotaniguchiOnalenna KereilweShinichi KitamuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroya Kadokawa Miyako Kotaniguchi Onalenna Kereilwe Shinichi Kitamura Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains
description	Abstract Ethanolamine plasmalogens (EPls), unique alkenylacyl-glycerophospholipids, are the only known ligands of G-protein-coupled receptor 61—a novel receptor co-localised with gonadotropin-releasing hormone receptors on anterior pituitary gonadotrophs. Brain EPl decreases with age. Commercial EPl—extracted from the cattle brain (unidentified age)—can independently stimulate FSH secretion from gonadotrophs. We hypothesised that there exists an age-related difference in the quality, quantity, and ability of bovine brain EPls to stimulate bovine gonadotrophs. We compared the brains of young (about 26 month old heifers) and old (about 90 month old cows) Japanese Black bovines, including EPls obtained from both groups. Additionally, mRNA expressions of the EPl biosynthesis enzymes, glyceronephosphate O-acyltransferase, alkylglycerone phosphate synthase, and fatty acyl-CoA reductase 1 (FAR1) were evaluated in young and old hypothalami. The old-brain EPl did not stimulate FSH secretion from gonadotrophs, unlike the young-brain EPl. Molecular species of EPl were compared using two-dimensional liquid chromatography-mass spectrometry. We identified 20 EPl molecular species of which three and three exhibited lower (P < 0.05) and higher (P < 0.05) ratios, respectively, in old compared to young brains. In addition, quantitative reverse transcription-polymerase chain reaction detected higher FAR1 levels in the POA, but not in the ARC&ME tissues, of old cows than that of fertile young heifers. Therefore, old-brain EPl may be associated with age-related infertility.
format	article
author	Hiroya Kadokawa Miyako Kotaniguchi Onalenna Kereilwe Shinichi Kitamura
author_facet	Hiroya Kadokawa Miyako Kotaniguchi Onalenna Kereilwe Shinichi Kitamura
author_sort	Hiroya Kadokawa
title	Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains
title_short	Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains
title_full	Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains
title_fullStr	Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains
title_full_unstemmed	Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains
title_sort	reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0cd4c556ea644850bb345b9d0325fc9a
work_keys_str_mv	AT hiroyakadokawa reducedgonadotrophstimulationbyethanolamineplasmalogensinoldbovinebrains AT miyakokotaniguchi reducedgonadotrophstimulationbyethanolamineplasmalogensinoldbovinebrains AT onalennakereilwe reducedgonadotrophstimulationbyethanolamineplasmalogensinoldbovinebrains AT shinichikitamura reducedgonadotrophstimulationbyethanolamineplasmalogensinoldbovinebrains
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Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains

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