Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane

Yili Qu1,3, Ping Wang1,3, Yi Man1, Yubao Li2, Yi Zuo2, Jidong Li21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064, China; 2Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, China; 3These authors contributed equally to...

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Autores principales:	Yili Qu, Ping Wang, Yi Man, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0cd7222647a74895bd8b4e0adc5d5b95
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spelling	oai:doaj.org-article:0cd7222647a74895bd8b4e0adc5d5b952021-12-02T02:49:26ZPreliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane1176-91141178-2013https://doaj.org/article/0cd7222647a74895bd8b4e0adc5d5b952010-06-01T00:00:00Zhttp://www.dovepress.com/preliminary-biocompatible-evaluation-of-nano-hydroxyapatitepolyamide-6-a4631https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yili Qu1,3, Ping Wang1,3, Yi Man1, Yubao Li2, Yi Zuo2, Jidong Li21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064, China; 2Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, China; 3These authors contributed equally to this workAbstract: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) composite with good bioactivity and osteoconductivity was employed to develop a novel porous membrane with asymmetric structure for guided bone regeneration (GBR). In order to test material cytotoxicity and to investigate surface-dependent responses of bone-forming cells, the morphology, proliferation, and cell cycle of bone marrow stromal cells (BMSCs) of rats cultured on the prepared membrane were determined. The polygonal and fusiform shape of BMSCs was observed by scanning electronic microscopy (SEM). The proliferation of BMSCs cultured on nHA/PA66 membrane tested by the MTT method (MTT: [3-{4,5-dimethylthiazol-2yl}-2,5-diphenyl-2H-tetrazoliumbromide]) was higher than that of negative control groups for 1 and 4 days&rsquo; incubation and had no significant difference for 7 and 11 days&rsquo; culture. The results of cell cycle also suggested that the membrane has no negative influence on cell division. The nHA/PA66 membranes were then implanted into subcutaneous sites of nine Sprague Dawley rats. The wounds and implant sites were free from suppuration and necrosis in all periods. All nHA/PA66 membranes were surrounded by a fibrous capsule with decreasing thickness 1 to 8 weeks postoperatively. In conclusion, the results of the in vitro and in vivo studies reveal that nHA/PA66 membrane has excellent biocompatibility and indicate its use in guided tissue regeneration (GTR) or GBR.Keywords: hydroxyapatite/polyamide, barrier membrane, biocompatibility, guided bone regeneration Yili QuPing WangYi Manet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 429-435 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yili Qu Ping Wang Yi Man et al Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane
description	Yili Qu1,3, Ping Wang1,3, Yi Man1, Yubao Li2, Yi Zuo2, Jidong Li21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064, China; 2Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, China; 3These authors contributed equally to this workAbstract: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) composite with good bioactivity and osteoconductivity was employed to develop a novel porous membrane with asymmetric structure for guided bone regeneration (GBR). In order to test material cytotoxicity and to investigate surface-dependent responses of bone-forming cells, the morphology, proliferation, and cell cycle of bone marrow stromal cells (BMSCs) of rats cultured on the prepared membrane were determined. The polygonal and fusiform shape of BMSCs was observed by scanning electronic microscopy (SEM). The proliferation of BMSCs cultured on nHA/PA66 membrane tested by the MTT method (MTT: [3-{4,5-dimethylthiazol-2yl}-2,5-diphenyl-2H-tetrazoliumbromide]) was higher than that of negative control groups for 1 and 4 days&rsquo; incubation and had no significant difference for 7 and 11 days&rsquo; culture. The results of cell cycle also suggested that the membrane has no negative influence on cell division. The nHA/PA66 membranes were then implanted into subcutaneous sites of nine Sprague Dawley rats. The wounds and implant sites were free from suppuration and necrosis in all periods. All nHA/PA66 membranes were surrounded by a fibrous capsule with decreasing thickness 1 to 8 weeks postoperatively. In conclusion, the results of the in vitro and in vivo studies reveal that nHA/PA66 membrane has excellent biocompatibility and indicate its use in guided tissue regeneration (GTR) or GBR.Keywords: hydroxyapatite/polyamide, barrier membrane, biocompatibility, guided bone regeneration
format	article
author	Yili Qu Ping Wang Yi Man et al
author_facet	Yili Qu Ping Wang Yi Man et al
author_sort	Yili Qu
title	Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane
title_short	Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane
title_full	Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane
title_fullStr	Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane
title_full_unstemmed	Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane
title_sort	preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/0cd7222647a74895bd8b4e0adc5d5b95
work_keys_str_mv	AT yiliqu preliminarybiocompatibleevaluationofnanohydroxyapatitepolyamide66compositeporousmembrane AT pingwang preliminarybiocompatibleevaluationofnanohydroxyapatitepolyamide66compositeporousmembrane AT yiman preliminarybiocompatibleevaluationofnanohydroxyapatitepolyamide66compositeporousmembrane AT etal preliminarybiocompatibleevaluationofnanohydroxyapatitepolyamide66compositeporousmembrane
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Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane

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