Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of h...

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Autores principales:	Maria V. Shestovskaya, Svetlana A. Bozhkova, Julia V. Sopova, Mikhail G. Khotin, Mikhail S. Bozhokin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cartilage tissue engineering regeneration repair scaffolds stem cells Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0cd79291776d4681881b10dc67243015
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spelling	oai:doaj.org-article:0cd79291776d4681881b10dc672430152021-11-25T16:50:32ZMethods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering10.3390/biomedicines91116662227-9059https://doaj.org/article/0cd79291776d4681881b10dc672430152021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1666https://doaj.org/toc/2227-9059The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.Maria V. ShestovskayaSvetlana A. BozhkovaJulia V. SopovaMikhail G. KhotinMikhail S. BozhokinMDPI AGarticlecartilagetissue engineeringregenerationrepairscaffoldsstem cellsBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1666, p 1666 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cartilage tissue engineering regeneration repair scaffolds stem cells Biology (General) QH301-705.5
spellingShingle	cartilage tissue engineering regeneration repair scaffolds stem cells Biology (General) QH301-705.5 Maria V. Shestovskaya Svetlana A. Bozhkova Julia V. Sopova Mikhail G. Khotin Mikhail S. Bozhokin Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering
description	The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.
format	article
author	Maria V. Shestovskaya Svetlana A. Bozhkova Julia V. Sopova Mikhail G. Khotin Mikhail S. Bozhokin
author_facet	Maria V. Shestovskaya Svetlana A. Bozhkova Julia V. Sopova Mikhail G. Khotin Mikhail S. Bozhokin
author_sort	Maria V. Shestovskaya
title	Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering
title_short	Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering
title_full	Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering
title_fullStr	Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering
title_full_unstemmed	Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering
title_sort	methods of modification of mesenchymal stem cells and conditions of their culturing for hyaline cartilage tissue engineering
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0cd79291776d4681881b10dc67243015
work_keys_str_mv	AT mariavshestovskaya methodsofmodificationofmesenchymalstemcellsandconditionsoftheirculturingforhyalinecartilagetissueengineering AT svetlanaabozhkova methodsofmodificationofmesenchymalstemcellsandconditionsoftheirculturingforhyalinecartilagetissueengineering AT juliavsopova methodsofmodificationofmesenchymalstemcellsandconditionsoftheirculturingforhyalinecartilagetissueengineering AT mikhailgkhotin methodsofmodificationofmesenchymalstemcellsandconditionsoftheirculturingforhyalinecartilagetissueengineering AT mikhailsbozhokin methodsofmodificationofmesenchymalstemcellsandconditionsoftheirculturingforhyalinecartilagetissueengineering
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Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

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