Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China

Abstract In this study, a novel 27-locus system (now known as the SureID PanGlobal system), including 24 autosomal STRs (D3S1358, TH01, D21S11, D18S51, Penta E, D12S391, D6S1043, D2S1338, D1S1656, D2S441, D5S818, D13S317, D7S820, D19S433, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D16S539, D22S1045,...

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Autores principales:	Yaju Liu, Lihong Guo, Haiying Jin, Zheng Li, Rufeng Bai, Meisen Shi, Shuhua Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cd7ad819b264d028bd1d76b6530d738
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spelling	oai:doaj.org-article:0cd7ad819b264d028bd1d76b6530d7382021-12-02T15:05:59ZDevelopmental validation of a 6-dye typing system with 27 loci and application in Han population of China10.1038/s41598-017-04548-12045-2322https://doaj.org/article/0cd7ad819b264d028bd1d76b6530d7382017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04548-1https://doaj.org/toc/2045-2322Abstract In this study, a novel 27-locus system (now known as the SureID PanGlobal system), including 24 autosomal STRs (D3S1358, TH01, D21S11, D18S51, Penta E, D12S391, D6S1043, D2S1338, D1S1656, D2S441, D5S818, D13S317, D7S820, D19S433, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D16S539, D22S1045, SE33, D10S1248), two Y-chromosome markers (DYS391 and Y-indel) and the sex determining marker, Amelogenin was developed with six fluorescent dyes labeling. The included STR loci belonged to the core loci in the Combined DNA Index System (CODIS) and the European Standard Set (ESS) as well as some additional loci commonly used in commercial kits and national DNA databases. This paper describes the validation studies conducted with the SureID PanGlobal system using Applied Biosystems 3500 XL Genetic Analyzer for fragment length detection that included the analysis of the following parameters and aspects: PCR conditions, sensitivity, species specificity, inhibition, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated that the SureID PanGlobal system is reproducible, accurate, sensitive and robust for forensic application and databasing. Additionally, the whole cycling time of the system can finish within 65 minutes, which was developed specifically for rapid and reliable generation of DNA profiles obtained from blood, buccal swabs and forensic stains.Yaju LiuLihong GuoHaiying JinZheng LiRufeng BaiMeisen ShiShuhua MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yaju Liu Lihong Guo Haiying Jin Zheng Li Rufeng Bai Meisen Shi Shuhua Ma Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China
description	Abstract In this study, a novel 27-locus system (now known as the SureID PanGlobal system), including 24 autosomal STRs (D3S1358, TH01, D21S11, D18S51, Penta E, D12S391, D6S1043, D2S1338, D1S1656, D2S441, D5S818, D13S317, D7S820, D19S433, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D16S539, D22S1045, SE33, D10S1248), two Y-chromosome markers (DYS391 and Y-indel) and the sex determining marker, Amelogenin was developed with six fluorescent dyes labeling. The included STR loci belonged to the core loci in the Combined DNA Index System (CODIS) and the European Standard Set (ESS) as well as some additional loci commonly used in commercial kits and national DNA databases. This paper describes the validation studies conducted with the SureID PanGlobal system using Applied Biosystems 3500 XL Genetic Analyzer for fragment length detection that included the analysis of the following parameters and aspects: PCR conditions, sensitivity, species specificity, inhibition, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated that the SureID PanGlobal system is reproducible, accurate, sensitive and robust for forensic application and databasing. Additionally, the whole cycling time of the system can finish within 65 minutes, which was developed specifically for rapid and reliable generation of DNA profiles obtained from blood, buccal swabs and forensic stains.
format	article
author	Yaju Liu Lihong Guo Haiying Jin Zheng Li Rufeng Bai Meisen Shi Shuhua Ma
author_facet	Yaju Liu Lihong Guo Haiying Jin Zheng Li Rufeng Bai Meisen Shi Shuhua Ma
author_sort	Yaju Liu
title	Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China
title_short	Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China
title_full	Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China
title_fullStr	Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China
title_full_unstemmed	Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China
title_sort	developmental validation of a 6-dye typing system with 27 loci and application in han population of china
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0cd7ad819b264d028bd1d76b6530d738
work_keys_str_mv	AT yajuliu developmentalvalidationofa6dyetypingsystemwith27lociandapplicationinhanpopulationofchina AT lihongguo developmentalvalidationofa6dyetypingsystemwith27lociandapplicationinhanpopulationofchina AT haiyingjin developmentalvalidationofa6dyetypingsystemwith27lociandapplicationinhanpopulationofchina AT zhengli developmentalvalidationofa6dyetypingsystemwith27lociandapplicationinhanpopulationofchina AT rufengbai developmentalvalidationofa6dyetypingsystemwith27lociandapplicationinhanpopulationofchina AT meisenshi developmentalvalidationofa6dyetypingsystemwith27lociandapplicationinhanpopulationofchina AT shuhuama developmentalvalidationofa6dyetypingsystemwith27lociandapplicationinhanpopulationofchina
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Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China

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