Deregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism

Abstract Malignant pleural mesothelioma (MPM) is an aggressive human cancer and miRNAs can play a key role for this disease. In order to broaden the knowledge in this field, the miRNA expression was investigated in a large series of MPM to discover new pathways helpful in diagnosis, prognosis and th...

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Autores principales:	Chiara De Santi, Ombretta Melaiu, Alessandra Bonotti, Luciano Cascione, Gianpiero Di Leva, Rudy Foddis, Alfonso Cristaudo, Marco Lucchi, Marco Mora, Anna Truini, Andrea Tironi, Bruno Murer, Renzo Boldorini, Monica Cipollini, Federica Gemignani, Pierluigi Gasparini, Luciano Mutti, Stefano Landi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ce7498b7bb2420192228c3b04550780
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spelling	oai:doaj.org-article:0ce7498b7bb2420192228c3b045507802021-12-02T16:06:08ZDeregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism10.1038/s41598-017-02694-02045-2322https://doaj.org/article/0ce7498b7bb2420192228c3b045507802017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02694-0https://doaj.org/toc/2045-2322Abstract Malignant pleural mesothelioma (MPM) is an aggressive human cancer and miRNAs can play a key role for this disease. In order to broaden the knowledge in this field, the miRNA expression was investigated in a large series of MPM to discover new pathways helpful in diagnosis, prognosis and therapy. We employed nanoString nCounter system for miRNA profiling on 105 MPM samples and 10 healthy pleura. The analysis was followed by the validation of the most significantly deregulated miRNAs by RT-qPCR in an independent sample set. We identified 63 miRNAs deregulated in a statistically significant way. MiR-185, miR-197, and miR-299 were confirmed differentially expressed, after validation study. In addition, the results of the microarray analysis corroborated previous findings concerning miR-15b-5p, miR-126-3p, and miR-145-5p. Kaplan-Meier curves were used to explore the association between miRNA expression and overall survival (OS) and identified a 2-miRNA prognostic signature (Let-7c-5p and miR-151a-5p) related to hypoxia and energy metabolism respectively. In silico analyses with DIANA-microT-CDS highlighted 5 putative targets in common between two miRNAs. With the present work we showed that the pattern of miRNAs expression is highly deregulated in MPM and that a 2-miRNA signature can be a new useful tool for prognosis in MPM.Chiara De SantiOmbretta MelaiuAlessandra BonottiLuciano CascioneGianpiero Di LevaRudy FoddisAlfonso CristaudoMarco LucchiMarco MoraAnna TruiniAndrea TironiBruno MurerRenzo BoldoriniMonica CipolliniFederica GemignaniPierluigi GaspariniLuciano MuttiStefano LandiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chiara De Santi Ombretta Melaiu Alessandra Bonotti Luciano Cascione Gianpiero Di Leva Rudy Foddis Alfonso Cristaudo Marco Lucchi Marco Mora Anna Truini Andrea Tironi Bruno Murer Renzo Boldorini Monica Cipollini Federica Gemignani Pierluigi Gasparini Luciano Mutti Stefano Landi Deregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism
description	Abstract Malignant pleural mesothelioma (MPM) is an aggressive human cancer and miRNAs can play a key role for this disease. In order to broaden the knowledge in this field, the miRNA expression was investigated in a large series of MPM to discover new pathways helpful in diagnosis, prognosis and therapy. We employed nanoString nCounter system for miRNA profiling on 105 MPM samples and 10 healthy pleura. The analysis was followed by the validation of the most significantly deregulated miRNAs by RT-qPCR in an independent sample set. We identified 63 miRNAs deregulated in a statistically significant way. MiR-185, miR-197, and miR-299 were confirmed differentially expressed, after validation study. In addition, the results of the microarray analysis corroborated previous findings concerning miR-15b-5p, miR-126-3p, and miR-145-5p. Kaplan-Meier curves were used to explore the association between miRNA expression and overall survival (OS) and identified a 2-miRNA prognostic signature (Let-7c-5p and miR-151a-5p) related to hypoxia and energy metabolism respectively. In silico analyses with DIANA-microT-CDS highlighted 5 putative targets in common between two miRNAs. With the present work we showed that the pattern of miRNAs expression is highly deregulated in MPM and that a 2-miRNA signature can be a new useful tool for prognosis in MPM.
format	article
author	Chiara De Santi Ombretta Melaiu Alessandra Bonotti Luciano Cascione Gianpiero Di Leva Rudy Foddis Alfonso Cristaudo Marco Lucchi Marco Mora Anna Truini Andrea Tironi Bruno Murer Renzo Boldorini Monica Cipollini Federica Gemignani Pierluigi Gasparini Luciano Mutti Stefano Landi
author_facet	Chiara De Santi Ombretta Melaiu Alessandra Bonotti Luciano Cascione Gianpiero Di Leva Rudy Foddis Alfonso Cristaudo Marco Lucchi Marco Mora Anna Truini Andrea Tironi Bruno Murer Renzo Boldorini Monica Cipollini Federica Gemignani Pierluigi Gasparini Luciano Mutti Stefano Landi
author_sort	Chiara De Santi
title	Deregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism
title_short	Deregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism
title_full	Deregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism
title_fullStr	Deregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism
title_full_unstemmed	Deregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism
title_sort	deregulation of mirnas in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0ce7498b7bb2420192228c3b04550780
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Deregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism

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